PDCD4 抗体 (pSer457)
-
- 抗原 See all PDCD4 抗体
- PDCD4 (Programmed Cell Death 4 (PDCD4))
-
抗原表位
- pSer457
-
适用
- 人, 小鼠, 大鼠, 非洲爪蟾
-
宿主
- 小鼠
-
克隆类型
- 单克隆
-
标记
- This PDCD4 antibody is un-conjugated
-
应用范围
- Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Enzyme Immunoassay (EIA), Immunohistochemistry (Frozen Sections) (IHC (fro))
- 特异性
- This antibody is specific for Pdcd4 protein phosphorylated at Ser457. Expect a band approximately 62 kDa in size corresponding to phosphorylated Pdcd4 protein by western blotting in the appropriate cell lysate or extract. This phospho-specific monoclonal antibody reacts with Human Pdcd4 pS457 and shows minimal reactivity by ELISA against the non-phosphorylated form of the immunizing peptide.
- 交叉反应 （详细）
-
Species reactivity (expected):Mouse, Rat and Xenopus (100 %).
Species reactivity (tested):Human. - 产品特性
- Synonyms: H731, Programmed cell death protein 4, Neoplastic transformation inhibitor protein,Nuclear antigen H731-like
- 纯化方法
- Protein A Chromatography
- 克隆位点
- 9G6
- 亚型
- IgG1
- Top Product
- Discover our top product PDCD4 Primary Antibody
-
anti-Programmed Cell Death 4 (PDCD4) (C-Term) antibody
PDCD4 适用: 人 WB, ELISA, IHC, IP 宿主: 兔 Polyclonal unconjugated
anti-Programmed Cell Death 4 (PDCD4) (pSer457) antibodyPDCD4 适用: 人 WB, ELISA 宿主: 兔 Polyclonal unconjugated
anti-Programmed Cell Death 4 (PDCD4) (AA 1-100) antibodyPDCD4 适用: 人, 小鼠, 大鼠 WB, ELISA, IHC (p), IF (cc), IF (p), IHC (fro) 宿主: 兔 Polyclonal unconjugated
anti-Programmed Cell Death 4 (PDCD4) (AA 1-469) antibodyPDCD4 适用: 人 WB, ELISA, IHC, IHC (p) 宿主: 小鼠 Monoclonal K4C1 unconjugated
anti-Programmed Cell Death 4 (PDCD4) (pSer457) antibodyPDCD4 适用: 人, 小鼠, 大鼠, 非洲爪蟾 WB, ELISA, IHC 宿主: 小鼠 Monoclonal 9G6 unconjugated
anti-Programmed Cell Death 4 (PDCD4) (AA 1-469) antibodyPDCD4 适用: 人 WB, IF 宿主: 小鼠 Polyclonal unconjugated
anti-Programmed Cell Death 4 (PDCD4) (N-Term) antibodyPDCD4 适用: 人, 小鼠, 大鼠 WB, ELISA, IHC, IF, ICC 宿主: 兔 Polyclonal unconjugated
anti-Programmed Cell Death 4 (PDCD4) (pSer67) antibodyPDCD4 适用: 人, 小鼠, 大鼠 WB, ELISA, IF, ICC 宿主: 兔 Polyclonal unconjugated
anti-Programmed Cell Death 4 (PDCD4) (AA 230-330) antibodyPDCD4 适用: 人 WB, IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-Programmed Cell Death 4 (PDCD4) (Internal Region), (pSer457) antibody (Biotin)PDCD4 适用: 人, 小鼠, 大鼠, 非洲爪蟾 WB, ELISA, IHC 宿主: 小鼠 Monoclonal 9G6 Biotin
-
- 应用备注
-
ELISA: 1: 400,000. Western blot: 1: 1,000 - 1: 3,000. Immunohistochemistry on frozen sections: 1.25 - 2.5 μg/mL, we recommend the use of freshfrozen tissues. Attempts at staining paraffin-embedded formalin fixed tissues were negative.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. - 限制
- 仅限研究用
-
- 状态
- Liquid
- 浓度
- 1.0 mg/mL (by UV absorbance at 280 nm)
- 缓冲液
- 0.02 M Potassium Phosphate, 0.12 M Sodium Chloride, pH 7.2, 0.01 % Sodium Azide as preservative
- 储存液
- Sodium azide
- 注意事项
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 储存条件
- 4 °C/-20 °C
- 储存方法
-
Store the antibody at 2 °C to 8 °C up to one month or (in aliquots) at -20 °C to -70 °C forlonger. To minimize loss of volume (25μl) dilute 1: 10 by adding 225 μl of the buffer statedabove directly to the vial. Recap, mix thoroughly and briefly centrifuge to collect thevolume at the bottom of the vial. Use this intermediate dilution when calculating finaldilutions. Avoid repeated freezing and thawing. Centrifuge product if not completely clear after standing at room temperature.
Shelf life: one year from despatch. - 有效期
- 12 months
-
- 抗原
- PDCD4 (Programmed Cell Death 4 (PDCD4))
- 别名
- PDCD4 (PDCD4 产品)
- 别名
- H731 antibody, D19Ucla1 antibody, Ma3 antibody, Tis antibody, Dug antibody, cb45 antibody, pdcd4 antibody, sb:cb45 antibody, wu:fc84b08 antibody, h731 antibody, MGC69337 antibody, PDCD4 antibody, wu:fa55g03 antibody, wu:fb08a11 antibody, wu:fe01a02 antibody, zgc:66311 antibody, programmed cell death 4 antibody, programmed cell death 4b antibody, programmed cell death 4 L homeolog antibody, programmed cell death 4 (neoplastic transformation inhibitor) antibody, programmed cell death 4a antibody, PDCD4 antibody, Pdcd4 antibody, pdcd4b antibody, pdcd4.L antibody, pdcd4 antibody, pdcd4a antibody
- 背景
- Programmed cell death 4 (Pdcd4) is a novel tumor suppressor. Pdcd4 directly inhibits the helicase activity of eukaryotic translation initiation factor 4A (eIF4A), a component of the translation initiation complex. Pdcd4 also suppresses the transactivation of activator protein-1 (AP-1)- responsive promoters by c-Jun. Pdcd4 contains two Akt phosphorylation sites, one at Ser67 and the other at Ser457. The phosphorylation of Pdcd4 by Akt causes nuclear translocation of Pdcd4 and a significant decrease in the ability of Pdcd4 to interfere with the transactivation of AP-1-responsive promoters by c-Jun.Synonyms: H731, Neoplastic transformation inhibitor protein, Nuclear antigen H731-like, Programmed cell death protein 4
- 基因ID
- 27250
- UniProt
- Q53EL6
-
