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DYKDDDDK 标记 抗体

ABIN400788 产品详细信息, 供应商: Log in to see
抗原
适用
标记
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1
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宿主
小鼠
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110
34
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17
克隆类型 (克隆位点)
单克隆 ()
标记
非结合性
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16
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应用范围
Enzyme Immunoassay (EIA), Flow Cytometry (FACS), Immunoprecipitation (IP), Western Blotting (WB)
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免疫原 Synthetic peptide corresponding to the DYKDDDDK epitope tag peptide DYKDDDDK(Asp-Tyr-Lys-Asp-Asp-Asp-Asp-Lys) conjugated to KLH using maleimide. Remarks: Residues of Glycine and Cysteine were added to the termini to facilitatecoupling.
序列 DYKDDDDK
克隆位点 29E4-G7-H11
亚型 IgG2a
产品特性 Synonyms: DYKDDDDK Epitope Tag, ECS Epitope Tag, DYKDDDDK-Tag, ECS-tag, D-tag
纯化方法 Protein A Chromatography followed by extensive dialysis against the buffer.
别名 DYKDDDDK Epitope Tag
物质类 Tag
背景 Sequences that are easily recognized by tag-specific antibodies. Due to their small size, epitope tags do not affect the tagged protein's biochemical properties. Most often sequences encoding the epitope tag are included with target DNA at the time of cloning to produce fusion proteins containing the epitope tag sequence. This allows anti-epitope tag antibodies to serve as universal detection reagents for any tag containing protein produced by recombinant means. This means that anti-epitope tag antibodies are a useful alternative to generating specific antibodies to identify, immunoprecipitate or immunoaffinity purify a recombinant protein. The anti-epitope tag antibody is usually functional in a variety of antibody-dependent experimental procedures. Expression vectors producing epitope tag fusion proteins are available for a variety of host expression systems including bacteria, yeast, insect and mammalian cells.
研究领域 Tags/Labels
应用备注 This antibody is optimally suited for monitoring the expression of DYKDDDDK tagged fusionproteins. As such, this antibody can be used to identify fusion proteins containing theDYKDDDDK epitope. The antibody recognizes the epitope tag fused to either the amino- orcarboxy- termini of targeted proteins. The epitope tag peptide sequence was first derivedfrom the 11-amino-acid leader peptide of the gene-10 product from bacteriophage T7. DYKDDDDK is the most commonly used hydrophilic octapeptide tag. Recommended Dilutions:
限制 仅限研究用
状态 Liquid
浓度 1.0 mg/mL (by UV absorbance at 280 nm)
缓冲液 0.02 M Potassium Phosphate, 0.12 M Sodium Chloride, pH 7.2 containing 0.01 % (w/v) Sodium Azide as preservative.
储存液 Sodium azide
注意事项 This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
储存条件 4 °C/-20 °C
储存方法 Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer. Avoid repeated freezing and thawing.
Shelf life: one year from despatch.
有效期 12 months
厂商提供的图像
Western Blotting (WB) image for anti-DYKDDDDK 标记 抗体 (ABIN400788) anti-DYKDDDDK Tag antibody
Western Blotting (WB) image for anti-DYKDDDDK 标记 抗体 (ABIN400788) anti-DYKDDDDK Tag antibody (Image 2)
背景 Slootstra, Kuperus, Plückthun, Meloen: "Identification of new tag sequences with differential and selective recognition properties for the anti-FLAG monoclonal antibodies M1, M2 and M5." in: Molecular diversity, Vol. 2, Issue 3, pp. 156-64, 1997 (PubMed).

Chubet, Brizzard: "Vectors for expression and secretion of FLAG epitope-tagged proteins in mammalian cells." in: BioTechniques, Vol. 20, Issue 1, pp. 136-41, 1996 (PubMed).

Robeva, Woodard, Luthin, Taylor, Linden: "Double tagging recombinant A1- and A2A-adenosine receptors with hexahistidine and the FLAG epitope. Development of an efficient generic protein purification procedure." in: Biochemical pharmacology, Vol. 51, Issue 4, pp. 545-55, 1996 (PubMed).