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Neutrophil Cytosolic Factor 4, 40kDa (NCF4) (AA 1-101) 抗体

ABIN393677 产品详细信息, 供应商: Log in to see
抗原
  • p40phox
  • AI451400
  • NCF
  • P40PHOX
  • SH3PXD4
  • p40phox protein
  • neutrophil cytosolic factor 4
  • neutrophil cytosolic factor 4, 40kDa
  • p40phox
  • LOC100136241
  • Ncf4
  • NCF4
抗原表位
AA 1-101
19
9
9
4
4
4
3
3
2
2
2
2
2
1
1
1
1
1
1
1
1
适用

117
58
44
7
7
5
4
4
3
3
宿主
小鼠
91
20
10
克隆类型 (克隆位点)
单克隆 ()
标记
非结合性
3
3
3
2
2
2
2
2
2
2
2
2
2
2
1
1
1
应用范围
ELISA
75
40
33
26
23
5
4
3
3
2
2
1
Supplier
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Available images

免疫原 NCF4 (AAH02798, 1 a.a. ~ 101 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 K
克隆位点 3C10
亚型 IgG2a kappa
特异性 NCF4 (AAH02798, 1 a.a. ~ 101 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
纯化方法 This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.
别名 NCF4 (NCF4 Antibody 摘要)
背景 Synonyms: Serine protease inhibitor J6, 47 kDa heat shock protein, Collagen-binding protein, Colligin, Serpin H1,Hsp47, Cbp1, Serpinh1
分子量 39032 DA
基因ID 12406
研究领域 Phospho-specific antibodies, Cell Signaling, Protein Modifications, Cell Structure
应用备注 ELISA ~~ 1ug/ml~3ng/ml
说明

Background: The protein encoded by this gene is a cytosolic regulatory component of the superoxide-producing phagocyte NADPH-oxidase, a multicomponent enzyme system important for host defense. This protein is preferentially expressed in cells of myeloid lineage. It interacts primarily with neutrophil cytosolic factor 2 (NCF2/p67-phox) to form a complex with neutrophil cytosolic factor 1 (NCF1/p47-phox), which further interacts with the small G protein RAC1 and translocates to the membrane upon cell stimulation. This complex then activates flavocytochrome b, the membrane-integrated catalytic core of the enzyme system. The PX domain of this protein can bind phospholipid products of the PI(3) kinase, which suggests its role in PI(3) kinase-mediated signaling events. The phosphorylation of this protein was found to negatively regulate the enzyme activity. Alternatively spliced transcript variants encoding distinct isoforms have been observed.

限制 仅限研究用
缓冲液 PBS with 0.09% (w/v) sodium azide.
储存液 Sodium azide
注意事项 This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
储存条件 4 °C/-20 °C
储存方法 Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
有效期 6 months
厂商提供的图像
ELISA image for anti-Neutrophil Cytosolic Factor 4, 40kDa (NCF4) (AA 1-101) 抗体 (ABIN393677) Detection limit for recombinant GST tagged NCF4 is approximately 0.3 ng/mL as a captu...
背景 Bailey, Xie, Do, Montpetit, Diaz, Mohan, Keavney, Yusuf, Gerstein, Engert, Anand: "Variation at the NFATC2 locus increases the risk of thiazolidinedione-induced edema in the Diabetes REduction Assessment with ramipril and rosiglitazone Medication (DREAM) study." in: Diabetes Care, Vol. 33, Issue 10, pp. 2250-3, 2010 (PubMed).

Han, Lan, Park, Lee, Park, Ahn, Shin, Kang, Koo, Seo, Choi, Ahn, Chanock, Kim, Rothman, Kang: "Polymorphisms in innate immunity genes and risk of childhood leukemia." in: Human immunology, Vol. 71, Issue 7, pp. 727-30, 2010 (PubMed).

Rajaraman, Brenner, Neta, Pfeiffer, Wang, Yeager, Thomas, Fine, Linet, Rothman, Chanock, Inskip: "Risk of meningioma and common variation in genes related to innate immunity." in: Cancer epidemiology, biomarkers & prevention : a publication of the American Association for Cancer Research, cosponsored by the American Society of Preventive Oncology, Vol. 19, Issue 5, pp. 1356-61, 2010 (PubMed).

Roos, Kuhns, Maddalena, Bustamante, Kannengiesser, de Boer, van Leeuwen, Köker, Wolach, Roesler, Malech, Holland, Gallin, Stasia: "Hematologically important mutations: the autosomal recessive forms of chronic granulomatous disease (second update)." in: Blood cells, molecules & diseases, Vol. 44, Issue 4, pp. 291-9, 2010 (PubMed).

Segat, Brandão, Guimarães, Pontillo, Athanasakis, de Oliveira, Arraes, de Lima Filho, Crovella: "Polymorphisms in innate immunity genes and patients response to dendritic cell-based HIV immuno-treatment." in: Vaccine, Vol. 28, Issue 10, pp. 2201-6, 2010 (PubMed).