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Myeloid/lymphoid Or Mixed-Lineage Leukemia (Trithorax Homolog, Drosophila), Translocated To, 3 (MLLT3) (AA 471-502), (C-Term) 抗体

ABIN390128 产品详细信息, 供应商: Log in to see
抗原
  • fc39c11
  • wu:fc39c11
  • zgc:110210
  • AF9
  • YEATS3
  • 2210011H10Rik
  • 2610012I03Rik
  • 3830408D16Rik
  • Af9
  • D4Ertd321e
  • Af-9
  • myeloid/lymphoid or mixed lineage-leukemia translocation to 3 homolog (Drosophila)
  • myeloid/lymphoid or mixed-lineage leukemia (trithorax homolog, Drosophila); translocated to, 3
  • mllt3
  • MLLT3
  • Mllt3
抗原表位
AA 471-502, C-Term
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适用

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宿主

80
3
克隆类型 (克隆位点)
多克隆 ()
标记
非结合性
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应用范围
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
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免疫原 This AF9 (MLLT3) antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 471-502 AA from the C-terminal region of human AF9 (MLLT3).
克隆位点 RB2249-2250
亚型 Ig
特异性 This AF9 (MLLT3) antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 479~509 amino acids from the C-terminal region of human MLLT3.
预测反应 Mouse (Murine)
纯化方法 This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
别名 AF9 (MLLT3) (MLLT3 Antibody 摘要)
背景 The human AF9 gene is one of the most common fusion partner genes with the ALL1 gene at 11q23 (also called MLL), resulting in the t(9,11)(p22,q23). The AF9 gene is more than 100 kb, and 2 patient breakpoint cluster regions (BCRs) have been identified, BCR1 is within intron 4, previously called site A, whereas BCR2 or site B spans introns 7 and 8. Several different structural elements have been identified in AF9, including a colocalizing in vivo DNA topo II cleavage site and an in vitro DNase I hypersensitive (DNase 1 HS) site in intron 7 in BCR2. Reversibility experiments demonstrated a religation of the topo II cleavage sites. In addition, 2 scaffold associated regions (SARs) are located centromeric to the topo II and DNase I HS cleavage sites and border breakpoint regions in 2 leukemic cells lines: SAR1 is located in intron 4, whereas SAR2 encompasses parts of exons 5-7. The patient breakpoint regions of AF9 share the same structural elements as the MLL BCR. A DNA breakage and repair model for nonhomologous recombination between MLL and its partner genes, particularly AF9, has been proposed.
Synonyms: AF-9 protein
分子量 63367 DA
基因ID 4300
研究领域 Cancer, Cell Structure
应用备注 WB = 1:1000, IHC (p) = 1:50-100
限制 仅限研究用
状态 Liquid
浓度 2 mg/mL
缓冲液 PBS with 0.09 % (W/V) sodium azide
储存液 Sodium azide
注意事项 This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
储存条件 4 °C/-20 °C
储存方法 Maintain refrigerated at 2-8 °C for up to 6 months. For long term storage store at -20 °C in small aliquots to prevent freeze-thaw cycles.
有效期 6 months
厂商提供的图像
Western Blotting (WB) image for anti-Myeloid/lymphoid Or Mixed-Lineage Leukemia (Trithorax Homolog, Drosophila), Translocated To, 3 (MLLT3) (AA 471-502), (C-Term) 抗体 (ABIN390128) Western blot analysis of AF9 (MLLT3) Antibody (C-term K486) (ABIN390128) in 293 cell ...
Immunohistochemistry (IHC) image for anti-Myeloid/lymphoid Or Mixed-Lineage Leukemia (Trithorax Homolog, Drosophila), Translocated To, 3 (MLLT3) (AA 471-502), (C-Term) 抗体 (ABIN390128) Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary ant...
有引用在: Benedikt, Baltruschat, Scholz, Bursen, Arrey, Meyer, Varagnolo, Müller, Karas, Dingermann, Marschalek: "The leukemogenic AF4-MLL fusion protein causes P-TEFb kinase activation and altered epigenetic signatures." in: Leukemia, Vol. 25, Issue 1, pp. 135-44, 2011 (PubMed).

Bitoun, Oliver, Davies: "The mixed-lineage leukemia fusion partner AF4 stimulates RNA polymerase II transcriptional elongation and mediates coordinated chromatin remodeling." in: Human molecular genetics, Vol. 16, Issue 1, pp. 92-106, 2007 (PubMed).

背景 Strissel, Strick, Tomek, Roe, Rowley, Zeleznik-Le: "DNA structural properties of AF9 are similar to MLL and could act as recombination hot spots resulting in MLL/AF9 translocations and leukemogenesis." in: Human molecular genetics, Vol. 9, Issue 11, pp. 1671-9, 2000 (PubMed).

Iida, Seto, Yamamoto, Komatsu, Tojo, Asano, Kamada, Ariyoshi, Takahashi, Ueda: "MLLT3 gene on 9p22 involved in t(9;11) leukemia encodes a serine/proline rich protein homologous to MLLT1 on 19p13." in: Oncogene, Vol. 8, Issue 11, pp. 3085-92, 1993 (PubMed).

Nakamura, Alder, Gu, Prasad, Canaani, Kamada, Gale, Lange, Crist, Nowell: "Genes on chromosomes 4, 9, and 19 involved in 11q23 abnormalities in acute leukemia share sequence homology and/or common motifs." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 90, Issue 10, pp. 4631-5, 1993 (PubMed).