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PCNA 抗体 (AA 89-117)

Name: PCNA 抗体 (AA 89-117)
SKU: ABIN389345
Availability: OutOfStock

PCNA 适用: 人 WB, IF, FACS, IHC (p) 宿主: 兔 Polyclonal RB17485 unconjugated

Western blot analysis of PCNA Antibody (Center) (ABIN389345 and ABIN2839454) in Jurkat(lane 1), Hela(lane 2), 293(lane 3) cell line lysates (35 μg/lane).

Formalin-fixed and paraffin-embedded human hepatocarcinoma tissue reacted with PCNA antibody (Center) (ABIN389345 and ABIN2839454) , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.

IHC (p)

Confocal immunofluorescent analysis of PCNA Antibody (Center) (ABIN389345 and ABIN2839454) with Hela cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green).

PCNA Antibody (Center) (ABIN389345 and ABIN2839454) flow cytometric analysis of Hela cells (right histogram) compared to a negative control cell (left histogram).

FACS

4 images

产品编号 ABIN389345

发货至: 中国

Datasheet as PDF

抗原 See all PCNA 抗体

PCNA (Proliferating Cell Nuclear Antigen (PCNA))
抗原表位
16

16

16

15

13

10

8

6

3

3

3

3

2

2

2

2

2

2

1

1

1

1

1

1

1

1

1

1

1

1

1

1
AA 89-117
适用
216

153

152

57

32

28

23

19

19

18

18

14

11

11

7

5

5

4

4

4

4

3

3

3

2

2

2

2

2

1

1

1

1

1
人
宿主
142

87

3
兔
Compatible Secondaries
克隆类型
141

90

1
多克隆
标记
116

17

14

9

5

5

5

5

5

5

5

5

5

5

5

5

2

2

1

1

1

1

1

1

1

1

1

1

1

1
This PCNA antibody is un-conjugated
应用范围
202

68

64

60

59

48

48

40

31

16

15

11

5

4

3

2

2

2

2

1

1

1

1

1
Western Blotting (WB), Immunofluorescence (IF), Flow Cytometry (FACS), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

预测反应

B, C, Ha, Pr, M, Rat

纯化方法

This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.

免疫原

This PCNA antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 89-117 amino acids from the Central region of human PCNA.

克隆位点

RB17485

亚型

Ig Fraction

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应用备注

IF: 1:10~50. WB: 1:1000. IHC-P: 1:10~50. FC: 1:10~50

限制

仅限研究用
状态

Liquid

缓冲液

Purified polyclonal antibody supplied in PBS with 0.09 % (W/V) sodium azide.

储存液

Sodium azide

注意事项

This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

储存条件

4 °C,-20 °C

储存方法

Maintain refrigerated at 2-8 °C for up to 6 months. For long term storage store at -20 °C in small aliquots to prevent freeze-thaw cycles.

有效期

6 months
抗原

PCNA (Proliferating Cell Nuclear Antigen (PCNA))

别名

PCNA (PCNA 产品)

别名

PCNAR antibody, Pcna/cyclin antibody, pcna antibody, AO090003000846 antibody, 53/13 antibody, CG9193 antibody, DmPCNA antibody, Dmel\\CG9193 antibody, MUS209 antibody, Mus209 antibody, PCNA antibody, PCNA/mus209 antibody, Pcna antibody, dPCNA antibody, l(2)02448 antibody, l(2)56F antibody, l(2)56Fa antibody, l(2)s1534 antibody, cb16 antibody, fb36g03 antibody, wu:fa28e03 antibody, wu:fb36g03 antibody, pcna-A antibody, proliferating cell nuclear antigen antibody, DNA polymerase III sliding clamp antibody, Proliferating cell nuclear antigen antibody, Proliferating cell nuclear antigen-like antibody, proliferating cell nuclear antigen L homeolog antibody, proliferating cell nuclear antigen 1 antibody, PCNA antibody, Pcna antibody, pcna antibody, MMP_RS08810 antibody, ANI_1_512014 antibody, AOR_1_1500154 antibody, MGC53867 antibody, pcna.L antibody, pcna1 antibody, LOC106420145 antibody, pcn1 antibody

背景

PCNA is found in the nucleus and is a cofactor of DNA polymerase delta. This protein acts as a homotrimer and helps increase the processivity of leading strand synthesis during DNA replication. In response to DNA damage, this protein is ubiquitinated and is involved in the RAD6-dependent DNA repair pathway.

分子量

28769

基因ID

5111

NCBI登录号

NP_002583, NP_872590

UniProt

P12004

途径

Telomere Maintenance, DNA Damage Repair, Mitotic G1-G1/S Phases, DNA Replication, Synthesis of DNA, Autophagy