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SMURF1 抗体 (N-Term)

This 兔多克隆 antibody specifically detects SMURF1 in WB 和 IHC (p). It exhibits reactivity toward 人和小鼠. It has been mentioned in 4+ publications

Formalin-fixed and paraffin-embedded human Testis tissue reacted with hSMURF1-W81 (ABIN388828 and ABIN2850430) , which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. (SMURF1 抗体 (N-Term))

IHC (p)

All lanes : Anti-SMURF1 Antibody (N-term) at 1:2000 dilution Lane 1: MCF-7 whole cell lysate Lane 2: Hela whole cell lysate Lysates/proteins at 20 μg per lane. (SMURF1 抗体 (N-Term))

Formalin-fixed and paraffin-embedded human breast carcinoma tissue reacted with hSMURF1-W81 (ABIN388828 and ABIN2850430) , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. (SMURF1 抗体 (N-Term))

IHC (p)

4 images

(4 references)

产品编号 ABIN388828

发货至: 中国

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Quick Overview for SMURF1 抗体 (N-Term) (ABIN388828)

抗原

See all SMURF1 抗体

SMURF1 (SMAD Specific E3 Ubiquitin Protein Ligase 1 (SMURF1))

适用

人, 小鼠

宿主

兔

克隆类型

多克隆

标记

This SMURF1 antibody is un-conjugated

应用范围

Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

克隆位点

RB04101

Discover our top product SMURF1 Primary Antibody

抗原表位
8

7

7

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3

2

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1

1

1

1
AA 66-96, N-Term

预测反应

X

纯化方法

This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.

免疫原

This SMURF1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 66-96 amino acids from the N-terminal region of human SMURF1.

亚型

Ig Fraction
anti-SMAD Specific E3 Ubiquitin Protein Ligase 1 (SMURF1) (AA 165-268) antibody
SMURF1 适用: 人 WB, ELISA, IF, IHC (p), IP 宿主: 小鼠 Monoclonal 1D7 unconjugated

anti-SMAD Specific E3 Ubiquitin Protein Ligase 1 (SMURF1) (AA 198-374) antibody
SMURF1 适用: 人 WB, ELISA, IHC, IF 宿主: 兔 Polyclonal unconjugated

anti-SMAD Specific E3 Ubiquitin Protein Ligase 1 (SMURF1) antibody
SMURF1 适用: 人, 小鼠 WB, IF, IHC (p), EIA, IP 宿主: 小鼠 Monoclonal 1D7 unconjugated

anti-SMAD Specific E3 Ubiquitin Protein Ligase 1 (SMURF1) (AA 1-731) antibody
SMURF1 适用: 人 WB, PLA 宿主: 兔 Polyclonal unconjugated

anti-SMAD Specific E3 Ubiquitin Protein Ligase 1 (SMURF1) (AA 66-96) antibody
SMURF1 适用: 人, 小鼠 WB, IHC, IHC (p) 宿主: 兔 Polyclonal unconjugated

anti-SMAD Specific E3 Ubiquitin Protein Ligase 1 (SMURF1) (AA 165-269) antibody
SMURF1 适用: 人, 小鼠 WB, ELISA, IHC, IF, IHC (p), IP 宿主: 小鼠 Monoclonal 1D7 unconjugated

anti-SMAD Specific E3 Ubiquitin Protein Ligase 1 (SMURF1) (AA 711-740), (C-Term) antibody
SMURF1 适用: 人 WB, IHC (p) 宿主: 兔 Polyclonal RB4103 unconjugated

anti-SMAD Specific E3 Ubiquitin Protein Ligase 1 (SMURF1) (N-Term) antibody
SMURF1 适用: 人, 小鼠 WB, IHC (p), EIA 宿主: 兔 Polyclonal unconjugated

anti-SMAD Specific E3 Ubiquitin Protein Ligase 1 (SMURF1) (AA 165-268) antibody
SMURF1 适用: 人 WB, ELISA 宿主: 小鼠 Polyclonal unconjugated

anti-SMAD Specific E3 Ubiquitin Protein Ligase 1 (SMURF1) (AA 326-351), (Middle Region) antibody
SMURF1 适用: 人, 大鼠 WB, IHC, IF, FACS, ICC 宿主: 兔 Polyclonal unconjugated

应用备注

WB: 1:1000. WB: 1:2000. IHC-P: 1:10~50. IHC-P: 1:10~50

限制

仅限研究用
状态

Liquid

缓冲液

Purified polyclonal antibody supplied in PBS with 0.09 % (W/V) sodium azide.

储存液

Sodium azide

注意事项

This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

注意事项

Avoid freeze-thaw cycles.

储存条件

4 °C,-20 °C

储存方法

Maintain refrigerated at 2-8 °C for up to 6 months. For long term storage store at -20 °C in small aliquots.

有效期

6 months
He, Yang, Isales, Shi: "Glucocorticoid-induced leucine zipper (GILZ) antagonizes TNF-? inhibition of mesenchymal stem cell osteogenic differentiation." in: PLoS ONE, Vol. 7, Issue 3, pp. e31717, (2012) (PubMed).

Guo, Yamashita, Zhang, Zhou, Chen, Reynolds, Awad, Yanoso, Zhao, Schwarz, Zhang, Boyce, Xing: "Ubiquitin ligase Smurf1 mediates tumor necrosis factor-induced systemic bone loss by promoting proteasomal degradation of bone morphogenetic signaling proteins." in: The Journal of biological chemistry, Vol. 283, Issue 34, pp. 23084-92, (2008) (PubMed).

Schwamborn, Müller, Becker, Püschel: "Ubiquitination of the GTPase Rap1B by the ubiquitin ligase Smurf2 is required for the establishment of neuronal polarity." in: The EMBO journal, Vol. 26, Issue 5, pp. 1410-22, (2007) (PubMed).

Kaneki, Guo, Chen, Yao, Schwarz, Zhang, Boyce, Xing: "Tumor necrosis factor promotes Runx2 degradation through up-regulation of Smurf1 and Smurf2 in osteoblasts." in: The Journal of biological chemistry, Vol. 281, Issue 7, pp. 4326-33, (2006) (PubMed).
抗原

SMURF1 (SMAD Specific E3 Ubiquitin Protein Ligase 1 (SMURF1))

别名

SMURF1

背景

Members of the transforming growth factor-beta (TGFB) family signal through type I and type II serine/threonine-kinase receptors, which in turn activate the SMAD signaling pathway. Bone morphogenetic protein (BMP) receptors target SMAD1, SMAD5, and SMAD8, whereas receptors for activin and TGFB (e.g., ACVR1 and TGFBR1, respectively) target SMAD2 and SMAD3. Phosphorylation of these receptor-regulated SMADs induces their association with the common-partner SMAD, SMAD4. Smurf1, a HECT domain E3 ubiquitin ligase, regulates cell polarity and protrusive activity and is required to maintain the transformed morphology and motility of a tumor cell. Atypical protein kinase C-zeta (PKC2), an effector of the Cdc42/Rac1-PAR6 polarity complex, recruits Smurf1 to cellular protrusions, where it controlled the local level of RhoA. Smurf1 thus links the polarity complex to degradation of RhoA in lamellipodia and filopodia to prevent RhoA signaling during dynamic membrane movements.

分子量

86114

基因ID

57154

NCBI登录号

NP_001186776, NP_065162, NP_851994

UniProt

Q9HCE7

途径

Regulation of Cell Size