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His 标记 抗体

ABIN387901 产品详细信息, 供应商: Log in to see
抗原
  • AKR1B11
  • AKR1B12
  • ALDRLn
  • ARL-1
  • ARL1
  • HIS
  • HSI
  • aldo-keto reductase family 1, member B10 (aldose reductase)
  • AKR1B10
适用
标记
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18
3
1
宿主

398
177
27
23
4
4
3
1
克隆类型 (克隆位点)
多克隆 ()
标记
非结合性
58
44
35
15
13
11
9
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8
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7
7
7
7
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6
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4
3
3
3
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
应用范围
Western Blotting (WB)
528
372
160
106
77
75
66
26
12
11
9
7
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4
4
3
2
1
1
1
1
1
1
1
1
1
1
Supplier
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Available images

免疫原 Poly-HIS peptide were used to produced this antibody.
序列 HHHHHH
克隆位点 RB19955
亚型 Ig
特异性 Poly-HIS peptide were used to produced this antibody.
纯化方法 This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
别名 6 X His Tag
物质类 Tag
背景 Epitope tags consisting of short sequences recognized by well-characterized monoclonal antibodies have been widely used in the study of protein expression in various systems. The 6xHIS tag (HHHHHH), recognized by the monoclonal antibody clone 6AT18 provides an established example of this application. 6xHIS-tagged fusion proteins are easily purified from cell lysates by affinity chromatography using Nickel-Sepharose resin. Abgent's anti-6xHIS monoclonal antibody provides a simple solution to detect the expression of HIS-tagged fusion proteins in cells.
Synonyms: Poly-His, Hexa-HIS, Hexa-Histidine
分子量 1000 DA
应用备注 WB = 1:1000
限制 仅限研究用
状态 Liquid
浓度 2 mg/mL
缓冲液 PBS with 0.09 % (W/V) sodium azide
储存液 Sodium azide
注意事项 This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
储存条件 4 °C/-20 °C
储存方法 Maintain refrigerated at 2-8 °C for up to 6 months. For long term storage store at -20 °C in small aliquots to prevent freeze-thaw cycles.
有效期 6 months
厂商提供的图像
Western Blotting (WB) image for anti-His 标记 抗体 (ABIN387901) Western blot analysis of 6*His Tag Antibody (ABIN387901) in his-tag TBB1 protein (35 ...
有引用在: Anderson, Robson, Jiang, Malmirchegini, Fierobe, Lazazzera, Clubb: "Assembly of minicellulosomes on the surface of Bacillus subtilis." in: Applied and environmental microbiology, Vol. 77, Issue 14, pp. 4849-58, 2011 (PubMed).

背景 Hochuli, Döbeli, Schacher: "New metal chelate adsorbent selective for proteins and peptides containing neighbouring histidine residues." in: Journal of chromatography, Vol. 411, pp. 177-84, 1988 (PubMed).