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MBD3 抗体 (C-Term)

MBD3 适用: 人, 小鼠 WB, IHC (p) 宿主: 兔 Polyclonal RB02375 unconjugated
产品编号 ABIN387891
发货至: 中国
  • 抗原 See all MBD3 抗体
    MBD3 (Methyl-CpG Binding Domain Protein 3 (MBD3))
    抗原表位
    • 14
    • 9
    • 7
    • 6
    • 3
    • 3
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    AA 264-291, C-Term
    适用
    • 70
    • 28
    • 15
    • 3
    • 3
    • 3
    • 2
    • 2
    • 1
    • 1
    • 1
    人, 小鼠
    宿主
    • 59
    • 9
    • 2
    克隆类型
    • 55
    • 15
    多克隆
    标记
    • 42
    • 5
    • 4
    • 3
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    This MBD3 antibody is un-conjugated
    应用范围
    • 46
    • 29
    • 10
    • 6
    • 5
    • 5
    • 3
    • 2
    • 1
    • 1
    • 1
    • 1
    Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
    纯化方法
    This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
    免疫原
    This MBD3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 264-291 amino acids from the C-terminal region of human MBD3.
    克隆位点
    RB02375
    亚型
    Ig Fraction
    Top Product
    Discover our top product MBD3 Primary Antibody
  • 应用备注
    WB: 1:1000. IHC-P: 1:50~100
    限制
    仅限研究用
  • 状态
    Liquid
    缓冲液
    Purified polyclonal antibody supplied in PBS with 0.09 % (W/V) sodium azide.
    储存液
    Sodium azide
    注意事项
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    储存条件
    4 °C,-20 °C
    储存方法
    Maintain refrigerated at 2-8 °C for up to 6 months. For long term storage store at -20 °C in small aliquots to prevent freeze-thaw cycles.
    有效期
    6 months
  • 抗原
    MBD3 (Methyl-CpG Binding Domain Protein 3 (MBD3))
    别名
    MBD3 (MBD3 产品)
    别名
    xmbd3 antibody, MGC69548 antibody, MBD3 antibody, DKFZp459N1635 antibody, AI181826 antibody, AU019209 antibody, methyl-CpG binding domain protein 3 S homeolog antibody, methyl-CpG binding domain protein 3 antibody, mbd3.S antibody, mbd3 antibody, MBD3 antibody, Mbd3 antibody
    背景
    DNA methylation, or the addition of methyl groups to cytosine bases in the dinucleotide CpG, is imperative to proper development and regulates gene expression. The methylation pattern involves the enzymatic processes of methylation and demethylation. The demethylation enzyme was recently found to be a mammalian protein, which exhibits demethylase activity associated to a methyl-CpG-binding domain (MBD). The enzyme is able to revert methylated cytosine bases to cytosines within the particular dinucleotide sequence mdCpdG by catalyzing the cleaving of the methyl group as methanol. MeCP2 and MBD1 (PCM1) are first found to repress transcription by binding specifically to methylated DNA. MBD2 and MBD4 (also known as MED1) were later found to colocalize with foci of heavily methylated satellite DNA and believed to mediate the biological functions of the methylation signal. Surprisingly, MBD3 does not bind methylated DNA both in vivo and in vitro. MBD1, MBD2, MBD3, and MBD4 are found to be expressed in somatic tissues, but the expression of MBD1 and MBD2 is reduced or absent in embryonic stem cells, which are known to be deficient in MeCP1 activity. MBD4 have homology to bacterial base excision repair DNA N-glycosylases/lyases. In some microsatellite unstable tumors MBD4 is mutated at an exonic polynucleotide tract.
    分子量
    32844
    基因ID
    53615
    NCBI登录号
    NP_001268382, NP_001268383
    UniProt
    O95983
    途径
    Chromatin Binding
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