V5 Epitope 标记 抗体

ABIN387850 产品详细信息, 供应商: Log in to see
抗原
适用
标记
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克隆类型 (克隆位点)
多克隆 ()
标记
非结合性
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应用范围
Western Blotting (WB)
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选项
Supplier
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免疫原 KLH conjugated synthetic peptide encoding the V5 tag (GKPIPNPLLGLDST) was used as antigen.
序列 GKPIPNPLLG LDST
克隆位点 RB0413
亚型 Ig
特异性 KLH conjugated synthetic peptide encoding the V5 tag (GKPIPNPLLGLDST) was used as antigen.
纯化方法 This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
别名 V5-Tag
物质类 Tag
背景 Epitope tags consisting of short sequences recognized by well-characterizated antibodies have been widely used in the study of protein expression in various systems. The V5 epitope tag is is composed of a 14 residue peptide, GKPIPNPLLGLDST, derived from a small epitope (Pk) present on the P and V proteins of the paramyxovirus of simian virus 5 (SV5). Abgent's anti-V5 tag polyclonal antibody provides a simple solution to detect the expression of a V5-tagged protein in cells.
分子量 DA
研究领域 Tags/Labels
应用备注 WB = 1:1000
限制 仅限研究用
状态 Liquid
浓度 2 mg/mL
缓冲液 PBS with 0.09 % (W/V) sodium azide
储存液 Sodium azide
注意事项 This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
储存条件 4 °C/-20 °C
储存方法 Maintain refrigerated at 2-8 °C for up to 6 months. For long term storage store at -20 °C in small aliquots to prevent freeze-thaw cycles.
有效期 6 months
厂商提供的图像
 image for anti-V5 Epitope Tag antibody (ABIN387850) Lanes: 1:non-transfected cells. 2:V5-tagged empty plasmid. 3:protein A (V5-tagged). 4...
Immunohistochemistry (IHC) image for anti-V5 Epitope Tag antibody (ABIN387850) Western blot analysis of anti-V5 Tag Antibody (ABIN387850) in 12tag protein (35 µg/la...
背景 Dunn, ODowd, Randall: "Fine mapping of the binding sites of monoclonal antibodies raised against the Pk tag." in: Journal of immunological methods, Vol. 224, Issue 1-2, pp. 141-50, 1999 (PubMed).

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