Myc 标记 抗体

ABIN387746 产品详细信息, 供应商: Log in to see
抗原
适用
标记
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宿主
小鼠
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克隆类型 (克隆位点)
单克隆 ()
标记
非结合性
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应用范围
Western Blotting (WB)
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选项
Supplier
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Supplier Product No.
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免疫原 KLH conjugated synthetic peptide encoding c-Myc tag (AEEQKLISEEDLLRKRREQLKHKLE) was used as antigen.
序列 EQKLISEEDL
克隆位点 9E10
亚型 IgG1
特异性 KLH conjugated synthetic peptide encoding c-Myc tag (AEEQKLISEEDLLRKRREQLKHKLE) was used as antigen.
纯化方法 This antibody is purified through a protein G column, followed by dialysis against PBS.
物质类 Tag
背景 Epitope tags consisting of short sequences recognized by well-characterizated monoclonal antibodies have been widely used in the study of protein expression in various systems. The HA tag (YPYDVPDYA) and Myc Tag (AEEQKLISEEDLLRKRREQLKHKLE), recognized by monoclonal antibody clones 12CA5 and 9E10, respectively, are illustrative examples. Abgent's anti-Myc monoclonal antibody (Clone 9E10) provides a simple solution to detect the expression of a Myc-tagged protein in cells.
Synonyms: Tag from c-Myc protein
分子量 48 kDA
研究领域 Tags/Labels
应用备注 WB = 1:2, 000
限制 仅限研究用
状态 Liquid
浓度 0.5 mg/mL
缓冲液 PBS with 0.09 % (W/V) sodium azide
储存液 Sodium azide
注意事项 This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
储存条件 4 °C/-20 °C
储存方法 Maintain refrigerated at 2-8 °C for up to 6 months. For long term storage store at -20 °C in small aliquots to prevent freeze-thaw cycles.
有效期 6 months
厂商提供的图像
 image for anti-Myc Tag antibody (ABIN387746) Myc-tagged recombinant protein was expressed in COS7 cells. 20 ug of cell lysate was ...
有引用在: Wang, Zhang, Dou, Zhang, Chen, Yu, Ren: "Synergistic and independent actions of multiple terminal nucleotidyl transferases in the 3' tailing of small RNAs in Arabidopsis." in: PLoS genetics, Vol. 11, Issue 4, pp. e1005091, 2015 (PubMed).

Li, An, Li, Ma, Feng, Zhang, Guo: "DELLA Proteins Interact with FLC to Repress the Flowering Transition." in: Journal of integrative plant biology, 2015 (PubMed).

Hu, Yu, Zhou, Li, Xia, Luo, Wu: "A small GTPase?like protein fragment of Mycoplasma promotes tumor cell migration and proliferation in vitro via interaction with Rac1 and Stat3." in: Molecular medicine reports, Vol. 9, Issue 1, pp. 173-9, 2013 (PubMed).

Liu, Yan, Liu, Hong, Sun, Shi, Huang, Fang: "Complementation of HYPONASTIC LEAVES1 by double-strand RNA-binding domains of DICER-LIKE1 in nuclear dicing bodies." in: Plant physiology, Vol. 163, Issue 1, pp. 108-17, 2013 (PubMed).

Chu, Loughlin, Gaur, SenBanerjee, Jacob, Monson, Kent, Oranu, Ding, Ukomadu, Sadler: "UHRF1 phosphorylation by cyclin A2/cyclin-dependent kinase 2 is required for zebrafish embryogenesis." in: Molecular biology of the cell, Vol. 23, Issue 1, pp. 59-70, 2012 (PubMed).

Chu, Mir, Gao, Rosa, Monson, Sharma, Steet, Freeze, Lehrman, Sadler: "A zebrafish model of congenital disorders of glycosylation with phosphomannose isomerase deficiency reveals an early opportunity for corrective mannose supplementation." in: Disease models & mechanisms, Vol. 6, Issue 1, pp. 95-105, 2012 (PubMed).

Hakozaki, Yoda, Tohmonda, Furukawa, Hikata, Uchikawa, Takaishi, Matsumoto, Chiba, Horiuchi, Toyama: "Receptor activator of NF-kappaB (RANK) ligand induces ectodomain shedding of RANK in murine RAW264.7 macrophages." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 184, Issue 5, pp. 2442-8, 2010 (PubMed).

Satow, Shitashige, Jigami, Honda, Ono, Hirohashi, Yamada: "Traf2- and Nck-interacting kinase is essential for canonical Wnt signaling in Xenopus axis formation." in: The Journal of biological chemistry, Vol. 285, Issue 34, pp. 26289-94, 2010 (PubMed).

Kim, Kang, Nagaraj, Blancaflor, Mysore, Chapman: "Mutations in Arabidopsis fatty acid amide hydrolase reveal that catalytic activity influences growth but not sensitivity to abscisic acid or pathogens." in: The Journal of biological chemistry, Vol. 284, Issue 49, pp. 34065-74, 2009 (PubMed).

Kobayashi, Barnard, Said, Hong-Gonzalez, Corman, Ku, Doan, Gui, Elashoff, Cohen, Aronson: "Effect of low-fat diet on development of prostate cancer and Akt phosphorylation in the Hi-Myc transgenic mouse model." in: Cancer research, Vol. 68, Issue 8, pp. 3066-73, 2008 (PubMed).

Zemskov, Mikhailenko, Strickland, Belkin: "Cell-surface transglutaminase undergoes internalization and lysosomal degradation: an essential role for LRP1." in: Journal of cell science, Vol. 120, Issue Pt 18, pp. 3188-99, 2007 (PubMed).

Hang, Zemskov, Lorand, Belkin: "Identification of a novel recognition sequence for fibronectin within the NH2-terminal beta-sandwich domain of tissue transglutaminase." in: The Journal of biological chemistry, Vol. 280, Issue 25, pp. 23675-83, 2005 (PubMed).

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