DYKDDDDK 标记 抗体 (FITC)

ABIN387724 产品详细信息, 供应商: Log in to see
抗原
适用
标记
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宿主
小鼠
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克隆类型 (克隆位点)
单克隆 ()
标记
FITC
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应用范围
Flow Cytometry (FACS), Immunofluorescence (IF)
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选项
Supplier
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Supplier Product No.
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品牌 THE™
免疫原 A synthetic peptide DYKDDDDK coupled - KLH
序列 DYKDDDDK
克隆位点 5A8E5
亚型 IgG
特异性 THETM DYKDDDDK Tag Antibody [FITC], mAb, Mouse recognizes C-terminal, N-terminal, and internal DYKDDDDK tagged fusion proteins.
产品特性 This product is suitable for detecting the expression level of DYKDDDDK fusion proteins by various immunoassays. Absorption Wavelength: 492 nmEmission Wavelength: 518 nmFluorochrome/Protein Ratio: >3 moles FITC per mole antibody
纯化方法 Protein A affinity column
物质类 Tag
应用备注 Working concentrations for specific applications should be determined by the investigator. The appropriate concentrations may be affected by secondary antibody affinity, antigen concentration, the sensitivity of the method of detection, temperature, the length of the incubations, and other factors. The suitability of this antibody for applications other than those listed below has not been determined. The following concentration ranges are recommended starting points for this product.
Immunofluorescence (IF): 0.5-2 µg/mL
Flow cytometry: 0.5-2 µg for 1 x 10^6 cells
Other applications: user-optimized
限制 仅限研究用
状态 Lyophilized
浓度 0.5 mg/mL
缓冲液 PBS, pH 7.4, containing 1 % BSA and 0.01 % Sodium azide
储存液 Sodium azide
注意事项 WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.
注意事项 Avoid repeated freezing and thawing cycles.
储存条件 4 °C/-20 °C
储存方法 The antibody is stable in lyophilized form if stored at -20°C or below. The reconstituted antibody can be stored for 2-3 weeks at 2-8°C. For long term storage, aliquot and store at -20°C or below. Avoid repeated freezing and thawing cycles.
厂商提供的图像
Flow Cytometry (FACS) image for anti-DYKDDDDK Tag antibody (FITC) (ABIN387724) Flow cytometric analysis of transfected HEK-293 cell expressing HA-tagged protein usi...
有引用在: Pumroy, Nardozzi, Hart, Root, Cingolani: "Nucleoporin Nup50 stabilizes closed conformation of armadillo repeat 10 in importin ?5." in: The Journal of biological chemistry, Vol. 287, Issue 3, pp. 2022-31, 2012 (PubMed).

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