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His 标记 抗体 (FITC)

ABIN387722 产品详细信息, 供应商: Log in to see
抗原
  • AKR1B11
  • AKR1B12
  • ALDRLn
  • ARL-1
  • ARL1
  • HIS
  • HSI
  • aldo-keto reductase family 1, member B10 (aldose reductase)
  • AKR1B10
适用
标记
616
18
3
1
宿主
小鼠
397
178
27
23
4
4
3
1
克隆类型 (克隆位点)
单克隆 ()
标记
FITC
58
44
34
15
13
11
9
8
8
7
7
7
7
7
7
7
6
6
6
5
4
4
4
3
3
3
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
应用范围
ELISA, Western Blotting (WB)
528
371
160
106
77
75
66
26
12
11
9
7
7
6
6
5
4
4
4
3
2
1
1
1
1
1
1
1
1
1
1
Supplier
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品牌 THE™
免疫原 A synthetic peptide HHHHHH coupled - KLH
序列 HHHHHH
克隆位点 6G2A9
亚型 IgG
特异性 THETM His Tag Antibody [FITC], mAb, Mouserecognizes C-terminal, N-terminal, and internal His tagged fusion proteins.
产品特性 This product is suitable for detecting the expression level of His fusion proteins by various immunoassays.Absorption Wavelength: 492 nmEmission Wavelength: 518 nmFluorochrome/Protein Ratio: >3 moles FITC per mole antibody
纯化方法 Protein A affinity column
物质类 Tag
研究领域 Tags/Labels
应用备注 Working concentrations for specific applications should be determined by the investigator. The appropriate concentrations may be affected by secondary antibody affinity, antigen concentration, the sensitivity of the method of detection, temperature, the length of the incubations, and other factors. The suitability of this antibody for applications other than those listed below has not been determined. The following concentration ranges are recommended starting points for this product.
Immunofluorescence (IF): 0.1-2 µg/mL
Flow cytometry: 0.1-2 µg for 1 x 10^6 cells
Other applications: user-optimized
限制 仅限研究用
状态 Lyophilized
浓度 1 mg/mL
缓冲液 PBS, pH 7.4, containing 1 % BSA and 0.01 % Sodium azide
储存液 Sodium azide
注意事项 WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.
注意事项 Avoid repeated freezing and thawing cycles.
储存条件 4 °C/-20 °C
储存方法 The antibody is stable in lyophilized form if stored at -20°C or below. The reconstituted antibody can be stored for 2-3 weeks at 2-8°C. For long term storage, aliquot and store at -20°C or below. Avoid repeated freezing and thawing cycles.
厂商提供的图像
 image for anti-His 标记 抗体 (FITC) (ABIN387722) Loading: 1. 40 µg pig muscle cell lysate2. 40 µg fish muscle cell lysate3. 40 µg CHO ...
有引用在: Yang, Wahlmüller, Sarg, Furtmüller, Geiger: "A+-helix of protein C inhibitor (PCI) is a cell-penetrating peptide that mediates cell membrane permeation of PCI." in: The Journal of biological chemistry, Vol. 290, Issue 5, pp. 3081-91, 2015 (PubMed).

Jarmander, Janoschek, Lundh, Larsson, Gustavsson: "Process optimization for increased yield of surface-expressed protein in Escherichia coli." in: Bioprocess and biosystems engineering, Vol. 37, Issue 8, pp. 1685-93, 2014 (PubMed).

An, Budd, Kanost, Michel: "Characterization of a regulatory unit that controls melanization and affects longevity of mosquitoes." in: Cellular and molecular life sciences : CMLS, Vol. 68, Issue 11, pp. 1929-39, 2011 (PubMed).

Oliveira, Lindblad: "Novel insights into the regulation of LexA in the cyanobacterium Synechocystis sp. Strain PCC 6803." in: Journal of bacteriology, Vol. 193, Issue 15, pp. 3804-14, 2011 (PubMed).

Li, Wang, Zhou, Yang, Bhaskar, Hu, Sun, Miao: "Cloning and expression of a cellulase gene in the silkworm, Bombyx mori by improved Bac-to-Bac/BmNPV baculovirus expression system." in: Molecular biology reports, Vol. 37, Issue 8, pp. 3721-8, 2010 (PubMed).