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DYKDDDDK Tag 抗体

WB, ELISA, IP 宿主: 小鼠 Monoclonal 5A8E5 unconjugated
产品编号 ABIN387700
发货至: 中国
  • 抗原 See all DYKDDDDK Tag products
    DYKDDDDK Tag
    适用
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    宿主
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    小鼠
    克隆类型
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    单克隆
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    This DYKDDDDK Tag antibody is un-conjugated
    应用范围
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    Western Blotting (WB), ELISA, Immunoprecipitation (IP)
    品牌
    THE™
    序列
    DYKDDDDK
    特异性
    THETM DYKDDDDK Tag Antibody, mAb, Mouse recognizes C-terminal, N-terminal and internal tagged fusion proteins.
    产品特性
    Mouse Anti-DYKDDDDK-tag Monoclonal Antibody is a high-affinity monoclonal antibody that can be used to detect DYKDDDDK-tagged proteins.
    纯化方法
    Protein A affinity column
    免疫原
    A synthetic peptide (DYKDDDDK) coupled - KLH
    克隆位点
    5A8E5
    亚型
    IgG
  • 应用备注
    Working concentrations for specific applications should be determined by the investigator. The appropriate concentrations may be affected by secondary antibody affinity, antigen concentration, the sensitivity of the method of detection, temperature, the length of the incubations, and other factors. The suitability of this antibody for applications other than those listed below has not been determined. The following concentration ranges are recommended starting points for this product.
    ELISA: 0.05-0.2 µg/mL
    Western blot: 0.1-1.0 µg/mL
    Immunoprecipitation: 1 µg/mL
    Other applications: user-optimized
    限制
    仅限研究用
  • 状态
    Lyophilized
    缓冲液
    PBS, pH 7.4, containing 0.02 % Sodium azide
    储存液
    Sodium azide
    注意事项
    WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.
    注意事项
    Avoid repeated freezing and thawing cycles.
    储存条件
    4 °C/-20 °C
    储存方法
    The antibody is stable in lyophilized form if stored at -20°C or below. The reconstituted antibody can be stored for 2-3 weeks at 2-8°C. For long term storage, aliquot and store at -20°C or below.
  • Reuben, Phénix, Santos, Lapointe: "The WT hemochromatosis protein HFE inhibits CD8? T-lymphocyte activation." in: European journal of immunology, Vol. 44, Issue 6, pp. 1604-14, (2014) (PubMed).

    Maryon, Molloy, Kaplan: "Cellular glutathione plays a key role in copper uptake mediated by human copper transporter 1." in: American journal of physiology. Cell physiology, Vol. 304, Issue 8, pp. C768-79, (2013) (PubMed).

  • 抗原
    DYKDDDDK Tag
    Abstract
    DYKDDDDK Tag 产品
    物质类
    Tag
    背景
    The DYKDDDDK peptide is a small component of the epitope which does not appear to interfere with the bioactivity or the biodistribution of the recombinant protein. It has been used extensively as a general epitope tag in expression vectors.
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