DYKDDDDK 标记 抗体

ABIN387700 产品详细信息, 供应商: Log in to see
抗原
适用
标记
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宿主
小鼠
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克隆类型 (克隆位点)
单克隆 ()
标记
非结合性
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应用范围
Immunoprecipitation (IP), ELISA, Western Blotting (WB)
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选项
Supplier
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Supplier Product No.
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品牌 THE™
免疫原 A synthetic peptide (DYKDDDDK) coupled - KLH
序列 DYKDDDDK
克隆位点 5A8E5
亚型 IgG
特异性 THETM DYKDDDDK Tag Antibody, mAb, Mouse recognizes C-terminal, N-terminal and internal tagged fusion proteins.
产品特性 Mouse Anti-DYKDDDDK-tag Monoclonal Antibody is a high-affinity monoclonal antibody that can be used to detect DYKDDDDK-tagged proteins.
纯化方法 Protein A affinity column
物质类 Tag
背景 The DYKDDDDK peptide is a small component of the epitope which does not appear to interfere with the bioactivity or the biodistribution of the recombinant protein. It has been used extensively as a general epitope tag in expression vectors.
应用备注 Working concentrations for specific applications should be determined by the investigator. The appropriate concentrations may be affected by secondary antibody affinity, antigen concentration, the sensitivity of the method of detection, temperature, the length of the incubations, and other factors. The suitability of this antibody for applications other than those listed below has not been determined. The following concentration ranges are recommended starting points for this product.
ELISA: 0.05-0.2 µg/mL
Western blot: 0.1-1.0 µg/mL
Immunoprecipitation: 1 µg/mL
Other applications: user-optimized
限制 仅限研究用
状态 Lyophilized
缓冲液 PBS, pH 7.4, containing 0.02 % Sodium azide
储存液 Sodium azide
注意事项 WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.
注意事项 Avoid repeated freezing and thawing cycles.
储存条件 4 °C/-20 °C
储存方法 The antibody is stable in lyophilized form if stored at -20°C or below. The reconstituted antibody can be stored for 2-3 weeks at 2-8°C. For long term storage, aliquot and store at -20°C or below.
厂商提供的图像
 image for anti-DYKDDDDK Tag antibody (ABIN387700) Loading: Lane 1.N-terminal DYKDDDDK-fusion protein (400 ng) Lane 2.C-terminal DYKDDDD...
 image for anti-DYKDDDDK Tag antibody (ABIN387700) Loading: Lane 1-3 Multiple Tag (Purified) (ABIN1536315, 400 ng, 80 ng,16 ng) Lane 4. ...
 image for anti-DYKDDDDK Tag antibody (ABIN387700) Lane1: 1 µL E-coli lysate with DYKDDDDK-tag protein (MW: 57 kDa) and 2 µg Mouse Anti-...
有引用在: Reuben, Phénix, Santos, Lapointe: "The WT hemochromatosis protein HFE inhibits CD8? T-lymphocyte activation." in: European journal of immunology, Vol. 44, Issue 6, pp. 1604-14, 2014 (PubMed).

Maryon, Molloy, Kaplan: "Cellular glutathione plays a key role in copper uptake mediated by human copper transporter 1." in: American journal of physiology. Cell physiology, Vol. 304, Issue 8, pp. C768-79, 2013 (PubMed).

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