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GTPase NRas 抗体 (Internal Region)

NRAS 适用: 人, 大鼠, 小鼠, 犬 WB, IHC (p) 宿主: 山羊 Polyclonal unconjugated
产品编号 ABIN375000
发货至: 中国
  • 抗原 See all GTPase NRas (NRAS) 抗体
    GTPase NRas (NRAS)
    抗原表位
    • 11
    • 6
    • 4
    • 4
    • 3
    • 3
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Internal Region
    适用
    • 46
    • 20
    • 5
    • 2
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    人, 大鼠, 小鼠, 犬
    宿主
    • 44
    • 12
    • 1
    山羊
    克隆类型
    • 42
    • 15
    多克隆
    标记
    • 37
    • 3
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    This GTPase NRas antibody is un-conjugated
    应用范围
    • 46
    • 18
    • 9
    • 7
    • 7
    • 7
    • 4
    • 3
    • 1
    • 1
    • 1
    • 1
    Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
    序列
    C-RMKKLNSSDD GTQ
    特异性
    This antibody detects NRAS.
    交叉反应 (详细)
    Species reactivity (expected):Mouse, Rat and Canine (Dog).
    Species reactivity (tested):Human.
    纯化方法
    Ammonium Sulphate Precipitation followed by antigen Affinity Chromatography using the immunizing peptide.
    免疫原
    Synthetic peptide corresponding to internal sequence of Human NRAS (NP_002515.1). Genename: NRAS
    Top Product
    Discover our top product NRAS Primary Antibody
  • 应用备注
    Peptide ELISA: Antibody detection limit dilution 1/32000. Western Blot: 0.01-0.03 μg/mL. Approx.21 kDa band observed in Human lysates of cell lineA431. In transfected HEK293 transiently expressing NRAS bands of approx.21 kDa and21 kDa are observed. These bands are not observed in the non-transfected HEK293. Immunohistochemistry: 2-3 μg/mL. In paraffin embedded Human Small Intestine shows stainingof cytoplasm and membrane of enterocytes.
    Other applications not tested.
    Optimal dilutions are dependent on conditions and should be determined by the user.
    限制
    仅限研究用
  • 浓度
    0.5 mg/mL
    缓冲液
    Tris saline, pH 7.3 containing 0.02 % Sodium Azide as preservative and 0.5 % BSA as stabilizer.
    储存液
    Sodium azide
    注意事项
    This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    注意事项
    Avoid repeated freezing and thawing.
    储存条件
    4 °C/-20 °C
    储存方法
    Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.
  • 抗原
    GTPase NRas (NRAS)
    别名
    NRAS (NRAS 产品)
    别名
    NRAS antibody, AV095280 antibody, N-ras antibody, ALPS4 antibody, NRAS1 antibody, NS6 antibody, N-RAS antibody, fj99a10 antibody, wu:fj99a10 antibody, zgc:100761 antibody, zras-b1 antibody, H-RAS antibody, HRAS antibody, GTPase NRas antibody, neuroblastoma RAS viral (v-ras) oncogene homolog L homeolog antibody, NRAS proto-oncogene, GTPase antibody, neuroblastoma ras oncogene antibody, neuroblastoma RAS viral oncogene homolog antibody, Tsp_01117 antibody, nras.L antibody, Nras antibody, NRAS antibody, nras antibody
    背景
    NRAS is an oncogene encoding a membrane protein that shuttles between the Golgi apparatus and the plasma membrane. Ras proteins bind GDP/GTP and possess intrinsic GTPase activity.Synonyms: ALPS4, GTPase NRas, HRAS1, N-Ras, Transforming protein N-Ras, neuroblastoma RAS viral (v-ras) oncogene homolog
    分子量
    21 kDa (NP_002515.1).
    基因ID
    4893, 9606
    UniProt
    P01111
    途径
    p53 Pathway, MAPK Pathway, RTK signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Hepatitis C, Regulation of long-term Neuronal Synaptic Plasticity, VEGF Signaling
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