Peptide ELISA: Limit dilution 1: 16000. Western blot: 0.2-0.6 μg/mL. Approx 50 kDa band observed in lysates of cell lines HEPG2,HeLa and Jurkat (calculated MW of 46.0 kDa according to NP_003070.3). A 40 kDa band wasobserved in transfected HEK293 transiently expressing BAF57. This band is not observed inthe non-transfected HEK293. Full caption: A) HEK293 overexpressing BAF57 (RC209444) and probed with EABIN374349 (mocktransfection in first lane), tested by Origene. B) ABIN374349 staining (0.2 μg/mL) of Jurkat lysate (RIPA buffer, 35 μg total protein perlane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.
限制
仅限研究用
浓度
0.5 mg/mL
缓冲液
Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin
储存液
Sodium azide
注意事项
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
注意事项
Avoid repeated freezing and thawing.
储存条件
4 °C/-20 °C
储存方法
Store at 2 - 8 °C for up to one month or (in aliquots) at -20 °C.
抗原
SMARCE1
(SWI/SNF Related, Matrix Associated, Actin Dependent Regulator of Chromatin, Subfamily E, Member 1 (SMARCE1))