Peptide ELISA: Limit dilution 1: 4000. Western blot: Preliminary experiments gave an approx 35 kDa band in Testis and Brainlysates after 0.3 μg/mL antibody staining. Please note that currently we cannot find anexplanation in the literature for the band we observe given the calculated size of 225 kDaaccording to NP_038478. The 35 kDa band was successfully blocked by incubation with theimmunizing peptide. We would appreciate any feedback from people in the field - have anyresults been reported with other antibodies/lysates? Have any further splicevariants/modified forms been reported?Immunohistochemistry: 1-2 μg/mL. In paraffin embedded Human Small Intestine showsprimarily nuclear staining in a number of cells of the lamina propria. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.
限制
仅限研究用
浓度
0.5 mg/mL
缓冲液
Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin
储存液
Sodium azide
注意事项
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
注意事项
Avoid repeated freezing and thawing.
储存条件
4 °C/-20 °C
储存方法
Store at 2 - 8 °C for up to one month or (in aliquots) at -20 °C.
抗原
BAZ2B
(Bromodomain Adjacent To Zinc Finger Domain, 2B (BAZ2B))
BAZ2B may play a role in transcriptional regulation interacting with ISWI. It is expressed at varying levels in several tissues, whereas a smaller transcript was expressed specifically in testis.Synonyms: Bromodomain adjacent to zinc finger domain protein 2B, KIAA1476, hWALp4