ADAM17 抗体 (C-Term)
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- 抗原 See all ADAM17 抗体
- ADAM17 (ADAM Metallopeptidase Domain 17 (ADAM17))
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抗原表位
- C-Term
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适用
- 人, 大鼠, 小鼠
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宿主
- 山羊
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克隆类型
- 多克隆
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标记
- This ADAM17 antibody is un-conjugated
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应用范围
- Western Blotting (WB)
- 序列
- LQRQNRVDSK ETEC
- 特异性
- This antibody detects CD156b / ADAM17 at C-term.
- 交叉反应 （详细）
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Species reactivity (expected):Mouse and Rat.
Species reactivity (tested):Human. - 纯化方法
- Ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide
- 免疫原
- Peptide from C Terminus of the protein sequence according to NP_003174.3. Genename: ADAM17
- Top Product
- Discover our top product ADAM17 Primary Antibody
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anti-ADAM Metallopeptidase Domain 17 (ADAM17) (AA 226-473) antibody
ADAM17 适用: 人 WB, IHC, ICC, IP 宿主: 小鼠 Monoclonal C10 unconjugated
anti-ADAM Metallopeptidase Domain 17 (ADAM17) (AA 215-314) antibodyADAM17 适用: 人 WB, ELISA, IHC (p), PLA 宿主: 小鼠 Monoclonal 1F6 unconjugated
anti-ADAM Metallopeptidase Domain 17 (ADAM17) (AA 201-300) antibodyADAM17 适用: 人, 大鼠, 小鼠 WB, ELISA, FACS, IHC (p), IF (cc), IF (p), IHC (fro) 宿主: 兔 Polyclonal unconjugated
anti-ADAM Metallopeptidase Domain 17 (ADAM17) (AA 226-473) antibodyADAM17 适用: 人 WB, IHC, ICC, IP 宿主: 兔 Polyclonal unconjugated
anti-ADAM Metallopeptidase Domain 17 (ADAM17) (C-Term) antibodyVerified ADAM17 适用: 人 WB, ELISA 宿主: 山羊 Polyclonal unconjugated
anti-ADAM Metallopeptidase Domain 17 (ADAM17) (C-Term) antibodyKO Validated ADAM17 适用: 人 WB, IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-ADAM Metallopeptidase Domain 17 (ADAM17) antibodyADAM17 适用: 人, 大鼠, 小鼠 WB, FACS, IF (cc) 宿主: 兔 Monoclonal 9E7 unconjugated
anti-ADAM Metallopeptidase Domain 17 (ADAM17) (pThr735) antibodyADAM17 适用: 人, 大鼠, 小鼠 WB, ELISA, IHC, ICC, IF 宿主: 兔 Polyclonal unconjugated
anti-ADAM Metallopeptidase Domain 17 (ADAM17) (N-Term) antibodyADAM17 适用: 人 WB, FACS 宿主: 兔 Polyclonal unconjugated
anti-ADAM Metallopeptidase Domain 17 (ADAM17) (AA 215-315) antibodyADAM17 适用: 人 WB, ELISA, IHC, IHC (p), PLA 宿主: 小鼠 Monoclonal 1F6 unconjugated
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- 应用备注
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Peptide ELISA: Limit dilution 1: 128000. Western blot: 0.1-0.3 μg/mL. Approx 100 kDa band observed in human testis lysates(calculated MW of 93.0 kDa according to NP_003174.3).
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. - 限制
- 仅限研究用
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- 浓度
- 0.5 mg/mL
- 缓冲液
- Tris saline, pH ~7.3, 0.02 % Sodium Azide, 0.5 % BSA
- 储存液
- Sodium azide
- 注意事项
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 注意事项
- Avoid repeated freezing and thawing.
- 储存条件
- 4 °C/-20 °C
- 储存方法
- Store at 2 - 8 °C for up to one month or (in aliquots) at -20 °C.
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- 抗原
- ADAM17 (ADAM Metallopeptidase Domain 17 (ADAM17))
- 别名
- CD156b / ADAM17 (ADAM17 产品)
- 别名
- ADAM18 antibody, CD156B antibody, CSVP antibody, NISBD antibody, TACE antibody, CD156b antibody, Tace antibody, adam17 antibody, si:dkey-81b15.2 antibody, adam18 antibody, cd156b antibody, csvp antibody, tace antibody, fc20b01 antibody, wu:fc20b01 antibody, zgc:63886 antibody, ADAM metallopeptidase domain 17 antibody, a disintegrin and metallopeptidase domain 17 antibody, ADAM metallopeptidase domain 17b antibody, ADAM metallopeptidase domain 17 L homeolog antibody, ADAM metallopeptidase domain 17a antibody, ADAM17 antibody, Adam17 antibody, adam17b antibody, adam17.L antibody, adam17a antibody
- 背景
- ADAM17, also known as TNF alpha Convertase, or TACE, was first cloned from human epithelial cells. The ADAMs proteins are structurally similar, with a signal sequence, metalloprotease domain (inactive in some ADAMs), disintegrin domain, cystein rich domain, EGF like repeat, type I transmembrane and Cytoplasmic domain. A member of the metalloproteinase family containing disintegrin like domains (ADAMs), ADAM17 is known to process the TNF alpha trimer from the membrane attached precursor to the soluble form. ADAM17 contains the canonical HExxHxxxxxH zinc metalloproteinase motif, and has been shown to be proteolytically active, cleaving TNF precursor as well as TNF p75 receptor, myeloid precursor protein, amyloid plaque protein, L selectin and TGF alpha, making ADAM17 a "sheddase". ADAM17 contains a cleavage site for prohormone convertases (furin), and the majority of ADAM17 is secreted as activated enzyme, cleaved at the furin site. ADAM17 is known to associate with MAD2 via the Cytoplasmic domain, implying cellular regulation pathways. ADAM17 is expressed in a wide range of cells in culture. Three human ADAM17 sequences encode proteins with different cytoplasmic domains, as is the case for other ADAMs proteases. The 824 amino acid sequence encodes a 92,960 Dalton protein, the 807 AA sequence 91,003 Dalton, and the 694 AA sequence 78,543 Dalton predicted MW. Glycosylation and other post translational modifications increase the apparent molecular weight on PAGE gels. Mouse and rat ADAM17 sequences are both 827 AA, predicting 93,073 and 93,017 Dalton proteins, respectively. Reports have shown that ADAM17 expression in human breast tumor samples is an indicator of poor prognosis.Synonyms: CSVP, Disintegrin and metalloproteinase domain-containing protein 17, Snake venom-like protease, TACE, TNF-alpha convertase, TNF-alpha-converting enzyme
- 基因ID
- 6868
- NCBI登录号
- NP_003174
- UniProt
- P78536
- 途径
- Notch Signaling, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Response to Growth Hormone Stimulus
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