DKK4 抗体 (C-Term)
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- 抗原 See all DKK4 抗体
- DKK4 (Dickkopf Homolog 4 (Xenopus Laevis) (DKK4))
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抗原表位
- C-Term
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适用
- 人
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宿主
- 山羊
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克隆类型
- 多克隆
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标记
- This DKK4 antibody is un-conjugated
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应用范围
- Western Blotting (WB)
- 序列
- RQHARLRVCQ KIEKL
- 特异性
- This antibody detects Dickkopf-4 at C-term.
- 纯化方法
- Ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide
- 免疫原
- Peptide from C Terminus of the protein sequence according to NP_055235.1 Genename: DKK4
- Top Product
- Discover our top product DKK4 Primary Antibody
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anti-Dickkopf Homolog 4 (Xenopus Laevis) (DKK4) (AA 19-224) antibody
DKK4 适用: 人 WB, ELISA, IHC 宿主: 兔 Polyclonal unconjugated
anti-Dickkopf Homolog 4 (Xenopus Laevis) (DKK4) (AA 107-212) antibodyDKK4 适用: 人 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-Dickkopf Homolog 4 (Xenopus Laevis) (DKK4) (C-Term) antibodyDKK4 适用: 人 WB, IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-Dickkopf Homolog 4 (Xenopus Laevis) (DKK4) (AA 162-189) antibodyDKK4 适用: 人 WB, ELISA 宿主: 兔 Polyclonal unconjugated
anti-Dickkopf Homolog 4 (Xenopus Laevis) (DKK4) (AA 19-224) antibodyDKK4 适用: 人 ELISA, IHC 宿主: 兔 Polyclonal unconjugated
anti-Dickkopf Homolog 4 (Xenopus Laevis) (DKK4) (AA 7-36), (N-Term) antibodyDKK4 适用: 人 WB, IHC (p), EIA 宿主: 兔 Polyclonal unconjugated
anti-Dickkopf Homolog 4 (Xenopus Laevis) (DKK4) (AA 1-224) antibodyDKK4 适用: 人 WB 宿主: 兔 Polyclonal unconjugated
anti-Dickkopf Homolog 4 (Xenopus Laevis) (DKK4) (AA 1-224) antibodyDKK4 适用: 人 WB 宿主: 小鼠 Polyclonal unconjugated
anti-Dickkopf Homolog 4 (Xenopus Laevis) (DKK4) (C-Term) antibodyVerified DKK4 适用: 人 WB, ELISA 宿主: 山羊 Polyclonal unconjugated
anti-Dickkopf Homolog 4 (Xenopus Laevis) (DKK4) (AA 37-141) antibodyDKK4 适用: 小鼠 WB, IHC, ICC 宿主: 兔 Polyclonal unconjugated
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- 应用备注
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Peptide ELISA: Limit dilution 1: 32000. Western blot: 1-3 μg/mL. Approx 30-35 kDa band seen in Jurkat lysate.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. - 限制
- 仅限研究用
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- 浓度
- 0.5 mg/mL
- 缓冲液
- Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin
- 储存液
- Sodium azide
- 注意事项
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 注意事项
- Avoid repeated freezing and thawing.
- 储存条件
- 4 °C/-20 °C
- 储存方法
- Store at 2 - 8 °C for up to one month or (in aliquots) at -20 °C.
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- 抗原
- DKK4 (Dickkopf Homolog 4 (Xenopus Laevis) (DKK4))
- 别名
- Dickkopf-4 (DKK4) (DKK4 产品)
- 别名
- DKK-4 antibody, Dkk-4 antibody, RGD1563172 antibody, dickkopf WNT signaling pathway inhibitor 4 antibody, DKK4 antibody, Dkk4 antibody
- 背景
- DKK4, like DKK1, DKK2, and DKK3, possesses an N terminal signal peptide and 2 conserved cysteine-rich domains, which are separated by a linker region and contain 10 cysteine residues each. The second cysteine region has a putative lipid-binding function that may facilitate WNT/DKK interactions at the plasma membrane. The linker region contains 50 to 55 amino acids in DKK1, DKK2, and DKK4, whereas in DKK3 it contains only 12 amino acids. All DKKs have several potential sites for cleavage by furin-type proteases. Northern blot analysis detected no expression of DKK4, but RT-PCR analysis detected DKK4 expression in cerebellum, T cell, esophagus, and lung cDNA libraries. DKK4 blocks Xenopus Wnt8, Wnt3a, and Wnt2b, but not Dsh or Fz8, induction of a secondary axis in frog embryos, indicating that DKKs antagonize WNT function upstream of WNT receptors. DKK4, like DKK1, DKK2, and DKK3, possesses an N terminal signal peptide and 2 conserved cysteine-rich domains, which are separated by a linker region and contain 10 cysteine residues each. The second cysteine region has a putative lipid-binding function that may facilitate WNT/DKK interactions at the plasma membrane. The linker region contains 50 to 55 amino acids in DKK1, DKK2, and DKK4, whereas in DKK3 it contains only 12 amino acids. All DKKs have several potential sites for cleavage by furin-type proteases. Northern blot analysis detected no expression of DKK4, but RT-PCR analysis detected DKK4 expression in cerebellum, T cell, esophagus, and lung cDNA libraries. DKK4 blocks Xenopus Wnt8, Wnt3a, and Wnt2b, but not Dsh or Fz8, induction of a secondary axis in frog embryos, indicating that DKKs antagonize WNT function upstream of WNT receptors.Synonyms: Dickkopf-related protein 4, Dkk-4
- 基因ID
- 27121
- NCBI登录号
- NP_055235
- UniProt
- Q9UBT3
- 途径
- WNT signaling
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