DARPP32 抗体 (N-Term)
(8 references)-
- 抗原 See all DARPP32 (PPP1R1B) 抗体
- DARPP32 (PPP1R1B) (Protein Phosphatase 1, Regulatory (Inhibitor) Subunit 1B (PPP1R1B))
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抗原表位
- N-Term
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适用
- 人, 小鼠, 大鼠, Cow, 犬, 小鸡, 非洲爪蟾, 斑马鱼
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宿主
- 兔
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克隆类型
- 多克隆
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标记
- This DARPP32 antibody is un-conjugated
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应用范围
- Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (Frozen Sections) (IHC (fro))
- 特异性
- Specific for the ~32k DARPP-32 protein.
- 交叉反应 （详细）
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Species reactivity (expected):Bovine, Canine, Chicken, Human, Mouse, Xenopus and Zebrafish.
Species reactivity (tested):Rat. - 纯化方法
- Affinity Chromatography.
- 免疫原
- Peptide corresponding to amino acid residues from the N-terminal region of rat DARPP-32, conjugated to keyhole limpet hemocyanin (KLH).
- 亚型
- IgG
- Top Product
- Discover our top product PPP1R1B Primary Antibody
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anti-Protein Phosphatase 1, Regulatory (Inhibitor) Subunit 1B (PPP1R1B) (pThr34) antibody
PPP1R1B 适用: 人, 小鼠, 大鼠 WB, IHC, ELISA, IF, ICC 宿主: 兔 Polyclonal unconjugated
anti-Protein Phosphatase 1, Regulatory (Inhibitor) Subunit 1B (PPP1R1B) (AA 95-204) antibodyPPP1R1B 适用: 人 WB, ELISA, FACS 宿主: 小鼠 Monoclonal 2G6D9 unconjugated
anti-Protein Phosphatase 1, Regulatory (Inhibitor) Subunit 1B (PPP1R1B) (pThr75) antibodyPPP1R1B 适用: 人, 小鼠, 大鼠 WB, IHC, ELISA, IF, ICC 宿主: 兔 Polyclonal unconjugated
anti-Protein Phosphatase 1, Regulatory (Inhibitor) Subunit 1B (PPP1R1B) antibodyMicroarray verified PPP1R1B 适用: 人, 小鼠 IHC 宿主: 小鼠 Monoclonal MAB349172 unconjugated
anti-Protein Phosphatase 1, Regulatory (Inhibitor) Subunit 1B (PPP1R1B) antibodyMicroarray verified PPP1R1B 适用: 人, 小鼠 IHC 宿主: 小鼠 Monoclonal MAB211117 unconjugated
anti-Protein Phosphatase 1, Regulatory (Inhibitor) Subunit 1B (PPP1R1B) (AA 1-168) antibodyPPP1R1B 适用: 人 WB, ELISA, IF 宿主: 小鼠 Monoclonal 1A3 unconjugated
anti-Protein Phosphatase 1, Regulatory (Inhibitor) Subunit 1B (PPP1R1B) antibodyPPP1R1B 适用: 人, 小鼠, 大鼠, 猴 WB, IHC, ELISA, IF, ICC 宿主: 兔 Polyclonal unconjugated
anti-Protein Phosphatase 1, Regulatory (Inhibitor) Subunit 1B (PPP1R1B) (N-Term) antibodyPPP1R1B 适用: 人, 小鼠, 大鼠, Cow, 犬, 小鸡, 斑马鱼, Frog WB, IF 宿主: 兔 Polyclonal unconjugated
anti-Protein Phosphatase 1, Regulatory (Inhibitor) Subunit 1B (PPP1R1B) (N-Term) antibodyPPP1R1B 适用: 大鼠 WB, IHC 宿主: 兔 Polyclonal unconjugated
anti-Protein Phosphatase 1, Regulatory (Inhibitor) Subunit 1B (PPP1R1B) (AA 1-36), (N-Term) antibodyPPP1R1B 适用: 人, 小鼠, 大鼠 WB, IHC (p) 宿主: 兔 Polyclonal unconjugated
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- 应用备注
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Western Blot: 1/1000. Immunohistochemistry: 1/250.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. - 限制
- 仅限研究用
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- 状态
- Liquid
- 缓冲液
- 10 mM HEPES ( pH 7.5), 150 mM NaCl, 100 μg/mL BSA and 50 % Glycerol.
- 注意事项
- Avoid repeated freezing and thawing.
- 储存条件
- -20 °C
- 储存方法
- Store the antibody undiluted (in aliquots) at-20 °C.
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: "Involvement of the Striatal Medium Spiny Neurons of the Direct Pathway in the Motor Stimulant Effects of Phencyclidine." in: The international journal of neuropsychopharmacology, (2016) (PubMed).
: "Role for the obesity-related FTO gene in the cellular sensing of amino acids." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 110, Issue 7, pp. 2557-62, (2013) (PubMed).
: "Alpha4-containing GABAA receptors in the nucleus accumbens mediate moderate intake of alcohol." in: The Journal of neuroscience : the official journal of the Society for Neuroscience, Vol. 29, Issue 2, pp. 543-9, (2009) (PubMed).
: "Marked disparity between age-related changes in dopamine and other presynaptic dopaminergic markers in human striatum." in: Journal of neurochemistry, Vol. 87, Issue 3, pp. 574-85, (2003) (PubMed).
: "Lack of regulation of aromatic L-amino acid decarboxylase in intact bovine chromaffin cells." in: Journal of neurochemistry, Vol. 81, Issue 3, pp. 589-93, (2002) (PubMed).
: "Striatal dopaminergic and serotonergic markers in human heroin users." in: Neuropsychopharmacology : official publication of the American College of Neuropsychopharmacology, Vol. 24, Issue 5, pp. 561-7, (2001) (PubMed).
: "Quantitation of tyrosine hydroxylase protein in the locus coeruleus from postmortem human brain." in: Journal of neuroscience methods, Vol. 99, Issue 1-2, pp. 37-44, (2000) (PubMed).
: "Elevated levels of tyrosine hydroxylase in the locus coeruleus in major depression." in: Biological psychiatry, Vol. 46, Issue 9, pp. 1275-86, (1999) (PubMed).
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: "Involvement of the Striatal Medium Spiny Neurons of the Direct Pathway in the Motor Stimulant Effects of Phencyclidine." in: The international journal of neuropsychopharmacology, (2016) (PubMed).
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- 抗原
- DARPP32 (PPP1R1B) (Protein Phosphatase 1, Regulatory (Inhibitor) Subunit 1B (PPP1R1B))
- 别名
- DARPP32 (PPP1R1B 产品)
- 别名
- darpp32 antibody, darpp-32 antibody, Darpp-32 antibody, Darpp32 antibody, DARPP-32 antibody, DARPP32 antibody, AU040756 antibody, protein phosphatase 1 regulatory inhibitor subunit 1B antibody, protein phosphatase 1, regulatory (inhibitor) subunit 1B antibody, protein phosphatase 1 regulatory inhibitor subunit 1B S homeolog antibody, ppp1r1b antibody, PPP1R1B antibody, Ppp1r1b antibody, ppp1r1b.S antibody
- 背景
- DARPP-32 is a dopamine (DA) and cAMP-regulated ~32k phosphoprotein that is associated with dopaminoceptive neurons (Fienberg et al., 1998). The protein inhibits protein phosphatase I when it is phosphorylated on Thr34. In contrast, when DARPP-32 is phosphorylated on Thr75 the protein acts as an inhibitor of PKA (Bibb et al., 1999). Phosphorylation of DARPP-32 is thought to play a critical role in the regulation of dopaminergic neurotransmission. In addition, the activity of DARPP-32 is also thought to play important roles in the actions of alcohol, caffeine and Prozac® (Maldve et al., 2002, Lindskog et al., 2002, Svenningsson et al., 2002).Synonyms: DARPP-32, Dopamine- and cAMP-regulated neuronal phosphoprotein, FLJ20940, PPP1R1B, Protein phosphatase 1 regulatory subunit 1B
- 基因ID
- 84152
- NCBI登录号
- NP_001229393
- UniProt
- Q9UD71
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