CAMK2A 抗体 (pThr286)
(7 references)-
- 抗原 See all CAMK2A 抗体
- CAMK2A (Calcium/calmodulin-Dependent Protein Kinase II alpha (CAMK2A))
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抗原表位
- pThr286
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适用
- 人, 小鼠, 大鼠, 非洲爪蟾
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宿主
- 兔
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克隆类型
- 多克隆
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标记
- This CAMK2A antibody is un-conjugated
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应用范围
- Western Blotting (WB)
- 特异性
- Specific for the ~50k Alpha-CaM Kinase II and the ~60k Beta-CaM Kinase II proteins phosphorylated at Thr286. Immunolabeling is blocked by the lambda-phosphatase treatment.
- 交叉反应 （详细）
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Species reactivity (expected):Human, Mouse and Xenopus.
Species reactivity (tested):Rat. - 纯化方法
- Sequential Chromatography on phospho- and dephosphopeptide affinity columns.
- 免疫原
- Phosphopeptide corresponding to amino acid residues surrounding the phospho-Thr286 found in rat brain CaM Kinase II.
- 亚型
- IgG
- Top Product
- Discover our top product CAMK2A Primary Antibody
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anti-Calcium/calmodulin-Dependent Protein Kinase II alpha (CAMK2A) (AA 1-478) antibody
CAMK2A 适用: 大鼠 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-Calcium/calmodulin-Dependent Protein Kinase II alpha (CAMK2A) (AA 305-410) antibodyCAMK2A 适用: 人 WB, ELISA, IF 宿主: 小鼠 Monoclonal 2C4 unconjugated
anti-Calcium/calmodulin-Dependent Protein Kinase II alpha (CAMK2A) (AA 305-410) antibodyCAMK2A 适用: 人 WB, ELISA, IF, PLA 宿主: 小鼠 Monoclonal 2C11 unconjugated
anti-Calcium/calmodulin-Dependent Protein Kinase II alpha (CAMK2A) (AA 305-410) antibodyCAMK2A 适用: 人 WB, ELISA, IF 宿主: 小鼠 Monoclonal 2C12 unconjugated
anti-Calcium/calmodulin-Dependent Protein Kinase II alpha (CAMK2A) (AA 321-478) antibodyCAMK2A 适用: 人 WB, ELISA, IHC, IF 宿主: 兔 Polyclonal unconjugated
anti-Calcium/calmodulin-Dependent Protein Kinase II alpha (CAMK2A) antibodyMicroarray verified CAMK2A 适用: 人, 小鼠 IHC 宿主: 小鼠 Monoclonal MAB443776 unconjugated
anti-Calcium/calmodulin-Dependent Protein Kinase II alpha (CAMK2A) (AA 321-478) antibodyCAMK2A 适用: 人 ELISA, IHC, IF 宿主: 兔 Polyclonal unconjugated
anti-Calcium/calmodulin-Dependent Protein Kinase II alpha (CAMK2A) (pThr286) antibodyCAMK2A 适用: 人 WB, ELISA 宿主: 兔 Polyclonal unconjugated
anti-Calcium/calmodulin-Dependent Protein Kinase II alpha (CAMK2A) (pThr305) antibodyCAMK2A 适用: 人 WB, ELISA, IHC 宿主: 兔 Polyclonal unconjugated
anti-Calcium/calmodulin-Dependent Protein Kinase II alpha (CAMK2A) antibodyCAMK2A 适用: 人, 小鼠, 大鼠 ELISA, IHC 宿主: 兔 Polyclonal unconjugated
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- 应用备注
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Western Blot: 1/1000.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. - 限制
- 仅限研究用
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- 状态
- Liquid
- 缓冲液
- 10 mM HEPES ( pH 7.5), 150 mM NaCl, 100 μg/mL BSA and 50 % Glycerol.
- 注意事项
- Avoid repeated freezing and thawing.
- 储存条件
- -20 °C
- 储存方法
- Store the antibody undiluted (in aliquots) at-20 °C.
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: "Ca2+-independent activation of Ca2+/calmodulin-dependent protein kinase II bound to the C-terminal domain of CaV2.1 calcium channels." in: The Journal of biological chemistry, Vol. 288, Issue 7, pp. 4637-48, (2013) (PubMed).
: "Nonsynaptic NMDA receptors mediate activity-dependent plasticity of gap junctional coupling in the AII amacrine cell network." in: The Journal of neuroscience : the official journal of the Society for Neuroscience, Vol. 32, Issue 20, pp. 6747-59, (2012) (PubMed).
: "CaMKII autonomy is substrate-dependent and further stimulated by Ca2+/calmodulin." in: The Journal of biological chemistry, Vol. 285, Issue 23, pp. 17930-7, (2010) (PubMed).
: "Effective post-insult neuroprotection by a novel Ca(2+)/ calmodulin-dependent protein kinase II (CaMKII) inhibitor." in: The Journal of biological chemistry, Vol. 285, Issue 27, pp. 20675-82, (2010) (PubMed).
: "Acute and chronic dopamine receptor stimulation modulates AMPA receptor trafficking in nucleus accumbens neurons cocultured with prefrontal cortex neurons." in: The Journal of neuroscience : the official journal of the Society for Neuroscience, Vol. 28, Issue 16, pp. 4216-30, (2008) (PubMed).
: "Long term synaptic depression that is associated with GluR1 dephosphorylation but not alpha-amino-3-hydroxy-5-methyl-4-isoxazolepropionic acid (AMPA) receptor internalization." in: The Journal of biological chemistry, Vol. 283, Issue 48, pp. 33138-46, (2008) (PubMed).
: "Dual mechanism of a natural CaMKII inhibitor." in: Molecular biology of the cell, Vol. 18, Issue 12, pp. 5024-33, (2007) (PubMed).
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: "Ca2+-independent activation of Ca2+/calmodulin-dependent protein kinase II bound to the C-terminal domain of CaV2.1 calcium channels." in: The Journal of biological chemistry, Vol. 288, Issue 7, pp. 4637-48, (2013) (PubMed).
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- 抗原
- CAMK2A (Calcium/calmodulin-Dependent Protein Kinase II alpha (CAMK2A))
- 别名
- CAMK2A (CAMK2A 产品)
- 别名
- camk2 antibody, camk2b antibody, zgc:112538 antibody, zgc:123320 antibody, CAMKA antibody, CaMKII antibody, R74975 antibody, mKIAA0968 antibody, PK2CDD antibody, PKCCD antibody, calcium/calmodulin dependent protein kinase II alpha antibody, calcium/calmodulin dependent protein kinase (CaM kinase) II alpha S homeolog antibody, calcium/calmodulin-dependent protein kinase II alpha antibody, CAMK2A antibody, camk2a.S antibody, camk2a antibody, Camk2a antibody
- 背景
- Ca2+/calmodulin-dependent protein kinase II (CaM Kinase II) is a multi-functional calcium and calmodulin-dependent protein kinase that mediates cellular responses to a wide variety of intercellular signals (Kennedy, 1998, Schulman and Hanson, 1993). CaM Kinase II has been shown to regulate diverse cellular functions including synaptic plasticity, neurotransmitter synthesis and release, gene expression, ion channel function, carbohydrate metabolism, cytoskeletal function, and Ca2+-homeostasis (Gleason et al., 2003, Soderling, 2000, Hudmon and Schulman, 2002). Phosphorylation of Thr286 on the kinase produces an autonomously active form of CaM Kinase II (Meng et al., 2003, Picciotto et al., 1993). Autophosphorylation of Thr305 inhibits the activity CaM Kinase II. Phosphorylation at this site appears to reduce the association of CaM Kinase II with the PSD and reduce LTP and learning (Elgersma et al., 2002).Synonyms: CAMK2A, CAMK2B, CAMKA, CaM kinase II subunit alpha, CaM kinase II subunit beta, CaM-kinase II alpha chain, CaM-kinase II beta chain, CaMK-II subunit alpha, CaMK-II subunit beta, Calcium/calmodulin-dependent protein kinase type II, KIAA0968
- 基因ID
- 815
- NCBI登录号
- NP_741960
- UniProt
- Q9UQM7
- 途径
- WNT signaling, Interferon-gamma Pathway, Myometrial Relaxation and Contraction
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