Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (Frozen Sections) (IHC (fro))
特异性
Specific for the ~29k 14-3-3 protein phosphorylated at Ser58. Immunolabeling is blocked by the phosphopeptide used as antigen but not by the corresponding dephosphopeptide.
交叉反应 (详细)
Species reactivity (expected):Bovine, Canine, Chicken, Mouse, Sheep, Xenopus, Zebrafish and non-Human Primates. Species reactivity (tested):Human and Rat.
纯化方法
Sequential Chromatography on phospho- and dephosphopeptide affinity columns.
免疫原
Phosphopeptide corresponding to amino acid residues surrounding the phospho-Ser58 of rat 14-3-3 protein.
Western Blot: 1/1000. Immunohistochemistry: 1/500. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.
限制
仅限研究用
状态
Liquid
缓冲液
10 mM HEPES ( pH 7.5), 150 mM NaCl, 100 μg/mL BSA and 50 % Glycerol.
注意事项
Avoid repeated freezing and thawing.
储存条件
-20 °C
储存方法
Store the antibody undiluted (in aliquots) at-20 °C.
14-3-3 proteins are a family of highly conserved proteins that appear to have multiple roles in cell signaling (Bridges and Moorhead, 2005). The proteins are abundantly expressed in the brain and have been detected in the cerebrospinal fluid of patients with different neurological disorders (Berg et al., 2003). 14-3-3 proteins bind protein ligands that are typically phosphorylated on serine or threonine residues and regulate the functions of these binding partners by a number of different mechanisms (Silhan et al., 2004, Dougherty and Morrison, 2004). The14-3-3 proteins affect a diverse array of cellular processes including the cell cycle and transcription, signal transduction and intracellular trafficking. These functions of 14-3-3 proteins are facilitated by, if not dependent on, its dimeric structure. Recent work has demonstrated that the dimeric status of the 14-3-3 protein is regulated by site-specific serine phosphorylation (Woodcock et al., 2003).Synonyms: KCIP-1, Protein 1054, Protein kinase C inhibitor protein 1, YWHAB