GFP 抗体 (Green Fluorescent Protein) (AA 1-238)

Details for Product anti-GFP Antibody No. ABIN371877, 供应商: Log in to see
抗原
  • green fluorescent protein
  • gfp
抗原表位
AA 1-238, N-Term
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适用
Aequorea victoria
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1
1
宿主
小鼠
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16
9
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2
克隆类型 (克隆位点)
单克隆 ()
标记
This GFP antibody is un-conjugated
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应用范围
Enzyme Immunoassay (EIA), Western Blotting (WB)
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选项
Supplier
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Supplier Product No.
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免疫原 Recombinant GFP (1-238 aa) purified from E. coli
克隆位点 AT2G5
亚型 IgG2a
产品特性 Synonyms: Green fluorescent protein, GFP-Tag
纯化方法 Protein-G affinity chromatography
别名 GFP
物质类 Tag
背景 GFP, also known as green fluorescent protein, is a protein produced by the jellyfish (Aequorea Victoria) that emits bioluminescence in the green zone of the visible spectrum. GFP has become a useful and ubiquitous tool for making chimeric proteins, where it functions as a fluorescent protein tag. It has been expressed in most known cell types and is used as a noninvasive fluorescent marker in living cells and organisms. This protein enables a wide range of applications where it has functioned as a cell lineage tracer, reporter of gene expression, or as a measure of protein-protein interactions.Synonyms: GFP-Tag, Green fluorescent protein
UniProt P42212
研究领域 Tags/Labels
应用备注 ELISA. Western blot (1: 1,000 - 1: 2,000).
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
限制 仅限研究用
状态 Liquid
浓度 1.0 mg/mL
缓冲液 PBS, pH 7.4, containing 0.09 % sodium azide
储存液 Sodium azide
注意事项 This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
储存条件 4 °C/-20 °C
储存方法 Store the antibody undiluted at 2-8 °C for up to two weeks or (in aliquots) at -20 °C forlonger. Avoid repeated freezing and thawing.
Shelf life: one year from despatch.
有效期 12 months
厂商提供的图像
Western Blotting (WB) image for anti-Green Fluorescent Protein (GFP) (AA 1-238), (N-Term) antibody (ABIN371877) anti-Green Fluorescent Protein (GFP) (AA 1-238), (N-Term) antibody
Western Blotting (WB) image for anti-Green Fluorescent Protein (GFP) (AA 1-238), (N-Term) antibody (ABIN371877) anti-Green Fluorescent Protein (GFP) (AA 1-238), (N-Term) antibody (Image 2)
背景 Phillips: "Green fluorescent protein--a bright idea for the study of bacterial protein localization." in: FEMS microbiology letters, Vol. 204, Issue 1, pp. 9-18, 2001 (PubMed).

Tsien: "The green fluorescent protein." in: Annual review of biochemistry, Vol. 67, pp. 509-44, 1998 (PubMed).

Prasher, Eckenrode, Ward, Prendergast, Cormier: "Primary structure of the Aequorea victoria green-fluorescent protein." in: Gene, Vol. 111, Issue 2, pp. 229-33, 1992 (PubMed).

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