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Mitogen-Activated Protein Kinase 1/3 (MAPK1/3) 抗体

ABIN371670 产品详细信息, 供应商: Log in to see
抗原
  • erk1/2
  • mitogen-activated protein kinase
  • erk1/2
适用

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宿主
小鼠
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75
6
克隆类型 (克隆位点)
单克隆 ()
标记
非结合性
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应用范围
Immunoprecipitation (IP), Western Blotting (WB)
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Supplier
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免疫原 Human recombinant Erk1 and Erk2.
克隆位点 SB46b
亚型 IgG2a
特异性 This antibody precipitates ~42 kDa and ~44 kDa bands, corresponding to Erk1 and Erk2, respectively.
产品特性 Synonyms: MAPK1, ERK2, PRKM1, PRKM2, ERK-2, ERT1, ERT2, PRKM3, MAPK3, ERK-1, ERK1,Mitogen-activated protein kinase, Extracellular signal-regulated kinase, Insulin-stimulatedMAP2 kinase
纯化方法 Purified
别名 ERK1 / ERK2 (MAPK1/3 Antibody 摘要)
背景 Erk1 and Erk2 are closely related mitogen activated protein (MAP) kinases which are activated by many growth factors, mitogens and differentiation-promoting agents via a protein kinase cascade. Also known as extracellular signal-regulated kinase 1 and 2, p44/p42 MAP kinases, microtubule-associated protein-2 kinases, myelin basic protein (MBP) kinases or EGF receptor T669 (Ert) kinases. (Ref.1-4) Erk1 and Erk2 are ubiquitous and abundant, although their relative abundance in specific tissues may vary. The two kinases are nearly 85 % identical and have higher identity in the core regions involved in substrate binding. (Ref.4) Erk1 and Erk2 are activated approximately 1000-fold by phosphorylation within a Thr-Glu-Tyr motif in the activation loop on both threonine and tyrosine residues by Mek1 and Mek2. (Ref.4,5) Both sites must be phosphorylated for maximum activity. (Ref.1-4) These kinases in turn phosphorylate a variety of different substrates. Erk1 and Erk2 are found in the cytoplasm and are translocated to the nucleus upon activation. Erk1 and Erk2 target membrane proteins, cytosolic proteins, such as downstream kinases, and cytoskeletal proteins and nuclear proteins, such as transcription factors. Many of these substrates are important regulatory proteins. Erk1 and Erk2 represent proximal kinases in the classical kinase pathway which links growth and differentiation signals at the cell surface (through tyrosine kinases) with transcription in the nucleus. (Ref.1-3)Synonyms: ERK-1/ERK-2, Extracellular signal-regulated kinase, Insulin-stimulated MAP2 kinase, MAPK1/MAPK2, Mitogen-activated protein kinase, P42/P44-MAPK
研究领域 Signaling, Inflammation
应用备注 Western Blot: 1-2 μg/mL. Predicted Mol. Weight: 42 and 44 kDa. Immunoprecipitation: 10-15 μg.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
限制 仅限研究用
浓度 0.05 mg/mL
缓冲液 100 mM Borate buffered saline, pH 8.0 without preservatives.
储存液 Without preservative
储存条件 4 °C/-20 °C
储存方法 Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer. Avoid repeated freezing and thawing.
Shelf life: one year from despatch.
有效期 12 months
厂商提供的图像
Western Blotting (WB) image for anti-Mitogen-Activated Protein Kinase 1/3 (MAPK1/3) 抗体 (ABIN371670) anti-Mitogen-Activated Protein Kinase 1/3 (MAPK1/3) antibody
背景 Xu Be, Stippec, Robinson, Cobb: "Hydrophobic as well as charged residues in both MEK1 and ERK2 are important for their proper docking." in: The Journal of biological chemistry, Vol. 276, Issue 28, pp. 26509-15, 2001 (PubMed).

Charest, Mordret, Harder, Jirik, Pelech: "Molecular cloning, expression, and characterization of the human mitogen-activated protein kinase p44erk1." in: Molecular and cellular biology, Vol. 13, Issue 8, pp. 4679-90, 1993 (PubMed).

Boulton, Yancopoulos, Gregory, Slaughter, Moomaw, Hsu, Cobb: "An insulin-stimulated protein kinase similar to yeast kinases involved in cell cycle control." in: Science (New York, N.Y.), Vol. 249, Issue 4964, pp. 64-7, 1990 (PubMed).