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小鼠 anti-人 IgE (Fc Region) Antibody

ELISA, WB, FACS, Func Monoclonal BSW17-NBS01 IgG1 kappa unconjugated
产品编号 ABIN363704
发货至: 中国
  • 抗原 See all IgE products
    IgE
    抗原表位
    • 30
    • 19
    • 17
    • 1
    • 1
    Fc Region
    适用
    • 48
    • 30
    • 10
    • 10
    • 5
    • 4
    • 2
    • 1
    宿主
    • 58
    • 18
    • 10
    • 10
    • 4
    • 1
    小鼠
    克隆类型
    • 66
    • 28
    单克隆
    应用范围
    • 55
    • 39
    • 30
    • 20
    • 20
    • 15
    • 12
    • 10
    • 10
    • 8
    • 8
    • 6
    • 5
    • 3
    • 3
    • 3
    • 1
    • 1
    • 1
    • 1
    ELISA, Western Blotting (WB), Flow Cytometry (FACS), Functional Studies (Func)
    特异性
    Human IgE, Fce3-4, no cross-ractivity with other human or murine Ab isotypes
    产品特性
    IGHE, chromosome 14q32.33
    纯化方法
    Affinity - purified from cell culture supernatant by Immunoaffinity Chromatography on CH-Sepharose 4B
    纯度
    > 95 %, tested by analytical Gel Permeation Chromatography
    免疫原
    purified human myeloma IgE
    克隆位点
    BSW17-NBS01
    亚型
    IgG1 kappa
  • 应用备注
    When used for functional cellular assays (mast cell / basophil activation) at dilutions < 1:100, the Na-azide free formulation is recommended. Quality assurance: Reactivity > 99 %, compared to calibrator (Internal Standard mAb BSW17), tested by ELISA binding to immobilized human Standard IgE (SUS-11)
    限制
    仅限研究用
  • 浓度
    1 mg/mL
    缓冲液
    PBS, pH=7.2, 0.1 % NaN3
    储存液
    Sodium azide
    注意事项
    WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.
    注意事项
    Monoclonal antibodies should not be stored at a temperature below -25 °C due to the aggregation effect of the protein.
    储存条件
    4 °C
  • Rudolf, Zuercher, Nechansky, Ruf, Vogel, Miescher, Stadler, Kricek: "Molecular basis for nonanaphylactogenicity of a monoclonal anti-IgE antibody." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 165, Issue 2, pp. 813-9, (2000) (PubMed).

  • 抗原
    IgE
    Abstract
    IgE 产品
    背景
    The antibody recognizes a conformational epitope within the Ce3 domain of human IgE spanning towards the amino-terminal part of the Ce4 domain. It blocks the binding of IgE to its high affinity receptor on mast cells and basophils. It is therefore suited as an inhibitory reference compound for mast cell modulator profiling and basic research on IgE mediated mast cell receptor signaling.
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