小鼠 anti-人 IgE (Fc Region) Antibody

ABIN363694 产品详细信息, 供应商: Log in to see
抗原
抗原表位
Fc Region
135
60
53
7
1
1
1
1
1
适用

489
141
46
44
6
6
3
2
2
2
1
1
1
宿主
小鼠
296
286
67
52
10
6
3
1
1
克隆类型 (克隆位点)
单克隆 ()
应用范围
Functional Studies (Func), Flow Cytometry (FACS), ELISA, Western Blotting (WB)
选项
Supplier
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Supplier Product No.
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免疫原 purified human myeloma IgE
克隆位点 Le27-NBS01
亚型 IgG1 kappa
特异性 Human IgE, Fce4, no cross-ractivity with other human or murine Ab isotypes
产品特性 IGHE, chromosome 14q32.33
纯化方法 Affinity - purified from cell culture supernatant by Immunoaffinity Chromatography on CH-Sepharose 4B
纯度 > 95 %, tested by analytical Gel Permeation Chromatography
过滤 Sterile filtered
背景 The antibody recognizes an epitope within the Ce4 domain of human IgE. It does not block the binding of IgE to its high affinity receptor on mast cells and basophils and is therefore suitable to detect receptor bound IgE. It can also be used as cross-linking agent for allergen-independent triggering of mast cells and basophils.
研究领域 Immunology, Secondary Antibodies
应用备注 For functional cellular assays (mast cell / basophil activation), in particular when used at dilutions less than 1:100 Quality assurance: Reactivity > 99 %, compared to calibrator (Internal Standard mAb Le27), tested by ELISA binding to immobilized human Standard IgE (SUS-11)
限制 仅限研究用
浓度 1 mg/mL
缓冲液 PBS, pH=7.2, sterile filtered, contains no NaN3
注意事项 Monoclonal antibodies should not be stored at a temperature below -25 °C due to the aggregation effect of the protein.
储存条件 4 °C
厂商提供的图像
 image for Mouse anti-Human IgE (Fc Region) antibody (ABIN363694) product purity: gel permeation chromatography (Superose 12/HR)
 image for Mouse anti-Human IgE (Fc Region) antibody (ABIN363694) Target binding kinetics (Biacore
有引用在: Rudolf, Zuercher, Nechansky, Ruf, Vogel, Miescher, Stadler, Kricek: "Molecular basis for nonanaphylactogenicity of a monoclonal anti-IgE antibody." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 165, Issue 2, pp. 813-9, 2000 (PubMed).

背景 Knutti-Mueller, Stadler, Magnusson, de Weck: "Human IgE synthesis in vitro. Detection with monoclonal antibodies." in: Allergy, Vol. 41, Issue 6, pp. 457-67, 1987 (PubMed).