The antibody detects endogenous level of total PKR protein.
纯化方法
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography usingepitope-specific immunogen.
免疫原
Peptide sequence around AA 449-453 (K-G-T-L-R) derived from Human PKR. Antibodies were produced by immunizing rabbits with synthetic peptide and KLH conjugates.
Following activation by double-stranded RNA in the presence of ATP, the kinase becomes autophosphorylated and can catalyze the phosphorylation of the translation initiation factor EIF2S1, which leads to an inhibition of the initiation of protein synthesis. Double-stranded RNA is generated during the course of a viral infection.