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NFKBIE 抗体 (pSer22)
NFKBIE
适用: 人, 小鼠
WB, IHC
宿主: 兔
Polyclonal
unconjugated
NFKBIE抗体详情
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抗原
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NFKBIE
(Nuclear Factor of kappa Light Polypeptide Gene Enhancer in B-Cells Inhibitor, epsilon (NFKBIE))
抗原表位
All epitopes for NFKBIE 抗体
pSer22
适用
All reactivities for NFKBIE 抗体
人, 小鼠
宿主
All hosts for NFKBIE 抗体
兔
克隆类型
All clonalities for NFKBIE 抗体
多克隆
标记
All conjugates for NFKBIE 抗体
This NFKBIE antibody is un-conjugated
应用范围
All applications for NFKBIE 抗体
Western Blotting (WB), Immunohistochemistry (IHC)
特异性
The antibody detects endogenous level of IkB-e only when phosphorylated at serine 22.
纯化方法
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
免疫原
Peptide sequence around phosphorylation site of pSer22 (I-E-S (p) -L-R) derived from Human IkB-e. Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates.
亚型
IgG
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使用细节
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应用备注
Western blotting: 1:500-1:1000 Immunohistochemistry: 1:50-1:100
限制
仅限研究用
贮存及处理
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状态
Liquid
浓度
1 mg/mL
缓冲液
Phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.
储存液
Sodium azide
注意事项
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
储存条件
4 °C/-20 °C
储存方法
Store at -20 °C for long term preservation (recommended). Store at 4 °C for short term use.
NFKBIE目标详情
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抗原
NFKBIE
(Nuclear Factor of kappa Light Polypeptide Gene Enhancer in B-Cells Inhibitor, epsilon (NFKBIE))
别名
IkB-epsilon (NFKBIE 产品 )
别名
NFKBIE antibody, IKBE antibody, Slc35b2 antibody, NFKB inhibitor epsilon antibody, nuclear factor of kappa light polypeptide gene enhancer in B-cells inhibitor, epsilon antibody, nuclear factor of kappa light polypeptide gene enhancer in B cells inhibitor, epsilon antibody, NFKBIE antibody, Nfkbie antibody
背景
Inhibits NF-kappa-B by complexing with and trapping it in the cytoplasm. Inhibits DNA-binding of NF-kappa-B p50-p65 and p50-c-Rel complexes.
分子量
40 kDa
NCBI登录号
NP_004547
UniProt
O00221
途径
NF-kappaB Signaling , Maintenance of Protein Location
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