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- 抗原 See all Phosphotyrosine products
- Phosphotyrosine
- 适用
- 请咨询
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宿主
- 兔
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克隆类型
- 多克隆
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标记
- This Phosphotyrosine antibody is conjugated to Biotin
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应用范围
- Western Blotting (WB), Immunoprecipitation (IP), Immunofluorescence (IF), Immunohistochemistry (IHC), ELISA, Immunocytochemistry (ICC)
- 特异性
- Detects proteins phosphorylated on tyrosine residues. Does not cross-react with phosphoserine or threonine.
- 纯化方法
- Peptide Affinity Purified
- 免疫原
- Phosphotyrosine conjugated to KLH
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anti-Phosphotyrosine antibody
WB, IP, IF, IHC, ELISA, ICC 宿主: 兔 Polyclonal unconjugated
anti-Phosphotyrosine antibodyWB, IP, IF, FACS, ELISA, ICC 宿主: 小鼠 Monoclonal E10 unconjugated
anti-Phosphotyrosine antibodyWB, IP, IHC, FACS, ELISA, ICC 宿主: 兔 Monoclonal RM111 unconjugated
anti-Phosphotyrosine antibody适用: Various Species WB, FACS, ICC 宿主: 小鼠 Monoclonal P-Tyr-01 unconjugated
anti-Phosphotyrosine antibody (FITC)WB, IP, IF, IHC, ELISA, ICC 宿主: 兔 Polyclonal FITC
anti-Phosphotyrosine antibody (Atto 488)WB, IP, IF, IHC, ELISA, ICC 宿主: 兔 Polyclonal Atto 488
anti-Phosphotyrosine antibody (Atto 594)WB, IP, IF, IHC, ELISA, ICC 宿主: 兔 Polyclonal Atto 594
anti-Phosphotyrosine antibody (Atto 390)WB, IP, IF, IHC, ELISA, ICC 宿主: 兔 Polyclonal Atto 390
anti-Phosphotyrosine antibodyWB, ELISA 宿主: 小鼠 Monoclonal unconjugated
anti-Phosphotyrosine antibody适用: Various Species WB, IHC (p) 宿主: 小鼠 Monoclonal unconjugated
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- 应用备注
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- WB (1:500)
- IHC (1:100)
- ICC/IF (1:50)
- ELISA (1:2000)
- IP (1:100)
- optimal dilutions for assays should be determined by the user.
- 说明
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A 1:250 dilution of ABIN361844 was sufficient for detection of tyrosine-phosphorylated species in mouse spleen lysates in western blot analysis.
- 限制
- 仅限研究用
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- 状态
- Liquid
- 浓度
- 0.25 mg/mL
- 缓冲液
- PBS pH 7.0, 50 % glycerol, 0.01 % sodium azide, Storage buffer may change when conjugated
- 储存液
- Sodium azide
- 注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 储存条件
- 4 °C
- 储存方法
- Conjugated antibodies should be stored at 4°C
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- 抗原
- Phosphotyrosine
- Abstract
- Phosphotyrosine 产品
- 物质类
- Amino Acid
- 背景
- Protein phosphorylation is an important posttranslational modification that serves many key functions to regulate a protein's activity, localization, and protein-protein interactions. Phosphorylation is catalyzed by various specific protein kinases, which involves removing a phosphate group from ATP and covalently attaching it to to a recipient protein that acts as a substrate. Most kinases act on both serine and threonine, others act on tyrosine, and a number (dual specificity kinases) act on all three. Because phosphorylation can occur at multiple sites on any given protein, it can therefore change the function or localization of that protein at any time (1). Changing the function of these proteins has been linked to a number of diseases, including cancer, diabetes, heart disease, inflammation and neurological disorders (2-4). In particular, the phosphorylation of tyrosine is considered one of the key steps in signal transduction and regulation of enzymatic activity (5). Phosphotyrosine can be detected through specific antibodies, and are helpful in facilitating the identification of tyrosine kinase substrates (6).
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