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Rab4 抗体 (C-Term)

RAB4A 适用: 人 WB, IHC, IF, ICC 宿主: 兔 Polyclonal unconjugated
产品编号 ABIN361840
发货至: 中国
  • 抗原 See all Rab4 (RAB4A) 抗体
    Rab4 (RAB4A) (RAB4A, Member RAS Oncogene Family (RAB4A))
    抗原表位
    • 16
    • 16
    • 7
    • 1
    • 1
    • 1
    C-Term
    适用
    • 68
    • 42
    • 24
    • 7
    • 4
    • 3
    • 3
    • 3
    • 3
    • 3
    • 3
    • 3
    • 3
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    宿主
    • 58
    • 8
    • 2
    克隆类型
    • 51
    • 17
    多克隆
    标记
    • 29
    • 4
    • 3
    • 3
    • 3
    • 3
    • 3
    • 3
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
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    This Rab4 antibody is un-conjugated
    应用范围
    • 50
    • 20
    • 17
    • 16
    • 15
    • 14
    • 14
    • 13
    • 5
    • 3
    • 1
    • 1
    • 1
    Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Immunocytochemistry (ICC)
    特异性
    Detects ~26 kDa.
    交叉反应
    人, 小鼠, 大鼠
    纯化方法
    Peptide Affinity Purified
    免疫原
    C-terminal peptide from human Rab4
    Top Product
    Discover our top product RAB4A Primary Antibody
  • 应用备注
    • WB (1:1000)
    • IHC (1:100)
    • ICC/IF (1:150)
    • optimal dilutions for assays should be determined by the user.
    说明

    A 1:1000 dilution of ABIN361840 was sufficient for detection of Rab4 in 10 μg of heat shocked HeLa cell lysate by colorimetric immunoblot analysis using Goat anti-rabbit IgG:HRP as the secondary antibody.

    限制
    仅限研究用
  • 状态
    Liquid
    浓度
    1 mg/mL
    缓冲液
    PBS pH 7.4, 50 % glycerol, 0.09 % sodium azide, Storage buffer may change when conjugated
    储存液
    Sodium azide
    注意事项
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    储存条件
    -20 °C
    储存方法
    -20°C
  • Ballmer-Hofer, Andersson, Ratcliffe, Berger: "Neuropilin-1 promotes VEGFR-2 trafficking through Rab11 vesicles thereby specifying signal output." in: Blood, Vol. 118, Issue 3, pp. 816-26, (2011) (PubMed).

  • 抗原
    Rab4 (RAB4A) (RAB4A, Member RAS Oncogene Family (RAB4A))
    别名
    Rab4 (RAB4A 产品)
    别名
    rab4a antibody, MGC52945 antibody, fc93b03 antibody, wu:fc93b03 antibody, RAB4A antibody, HRES-1/RAB4 antibody, RAB4 antibody, AI848268 antibody, Rab4 antibody, RAB4A, member RAS oncogene family antibody, RAB4a, member RAS oncogene family antibody, rab4a antibody, RAB4A antibody, Rab4a antibody
    背景
    Rab4 is a 25 kDa member of the Rab family of small guanosine triphosphatases (GTPases), Ras superfamily. Rab GTPases are central regulators of membrane trafficking in the eukaryotic cell. Their regulatory capacity depends on their ability to cycle between the GDP -bound inactive and GTP-bound active states. This conversion is regulated by GDP/GTP exchange factors (GEPs), GDP dissociation inhibitors (GDIs) and GTPase-activating proteins (GAPs) (1, 2). Activation of a Rab protein is coupled to its association with intracellular membranes, allowing it to recruit downstream effector proteins to the cytoplasmic surface of a sub-cellular compartment (3). Through these proteins, Rab GTPases regulate vesicle formation, actin- and tubulin-dependent vesicle movement, and membrane fusion(1). Rab proteins contain conserved regions involved in guanine-nucleotide binding, and hyper-variable COHO-terminal domains with a cysteine motif implicated in sub-cellular targeting. Post-translational modification of the cysteine motif with one or two geranylgeranyl groups is essential for the membrane association and correct intracellular localization of Rab proteins (3). Each Rab shows a characteristic sub-cellular distribution (4). In particular, over-expression of Rab4 causes a redistribution of receptors on plasma membrane versus endocytic compartments. The presence of excessive Rab4 leads to the accumulation of tranferrin receptors in non-acidic, post-endosomal recycling vesicles considered an intermediate compartment between endosomes and plasma membranes. Rab4 also plays a role in the translocation of glucose transporter (Glu4) in adipocytes in response to insulin (5). Mediating the association of Rab4 with transferring receptor-containing early endosomes takes place through the geranylgeranyl groups at its carboxyl-terminus. Membrane association is also cell cycle dependent, as phosphorylation at its c-terminal cdc2 kinase consensus sequence in mitotic cells leads to dissociation of Rab4 into the cytosol (6).
    基因ID
    5867
    NCBI登录号
    NP_004569
    UniProt
    P20338
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