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Phosphothreonine 抗体

WB, ELISA, IP, ICC, IF 宿主: 兔 Polyclonal unconjugated
产品编号 ABIN361757
发货至: 中国
  • 抗原 See all Phosphothreonine products
    Phosphothreonine
    宿主
    • 16
    • 7
    克隆类型
    • 14
    • 9
    多克隆
    标记
    • 14
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    This Phosphothreonine antibody is un-conjugated
    应用范围
    • 22
    • 19
    • 17
    • 7
    • 7
    • 5
    • 5
    • 3
    • 1
    • 1
    • 1
    Western Blotting (WB), ELISA, Immunoprecipitation (IP), Immunocytochemistry (ICC), Immunofluorescence (IF)
    特异性
    Detects proteins phosphorylated on threonine residues. Does not cross-react with phosphotyrosine.
    纯化方法
    Peptide Affinity Purified
    免疫原
    Phosphothreonine conjugated to KLH
  • 应用备注
    • WB (1:500)
    • ICC/IF (1:60)
    • ELISA (1:2000)
    • IP (1:100)
    • optimal dilutions for assays should be determined by the user.
    说明

    2 μg/ml of ABIN361757 was sufficient for detection of phosphorylation signal in western blot analysis using mouse spleen extract treated with Vanadium.

    限制
    仅限研究用
  • 状态
    Liquid
    浓度
    0.25 mg/mL
    缓冲液
    PBS, 50 % glycerol, 0.01 % sodium azide, Storage buffer may change when conjugated
    储存液
    Sodium azide
    注意事项
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    储存条件
    -20 °C
    储存方法
    -20°C
  • 抗原
    Phosphothreonine
    Abstract
    Phosphothreonine 产品
    物质类
    Amino Acid
    背景
    Protein phosphorylation is an important posttranslational modification that serves many key functions to regulate a protein's activity, localization, and protein-protein interactions. Phosphorylation is catalyzed by various specific protein kinases, which involves removing a phosphate group from ATP and covalently attaching it to to a recipient protein that acts as a substrate. Most kinases act on both serine and threonine, others act on tyrosine, and a number (dual specificity kinases) act on all three. Because phosphorylation can occur at multiple sites on any given protein, it can therefore change the function or localization of that protein at any time (1). Changing the function of these proteins has been linked to a number of diseases, including cancer, diabetes, heart disease, inflammation and neurological disorders (2-4).
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