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PUMA 抗体 (N-Term)

BBC3 适用: 人 WB, IHC 宿主: 兔 Polyclonal unconjugated
产品编号 ABIN361754
发货至: 中国
  • 抗原 See all PUMA (BBC3) 抗体
    PUMA (BBC3) (BCL2 Binding Component 3 (BBC3))
    抗原表位
    • 21
    • 17
    • 16
    • 10
    • 8
    • 7
    • 6
    • 6
    • 6
    • 6
    • 4
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    N-Term
    适用
    • 113
    • 40
    • 39
    • 16
    宿主
    • 109
    • 7
    • 1
    克隆类型
    • 98
    • 19
    多克隆
    标记
    • 52
    • 8
    • 8
    • 6
    • 5
    • 5
    • 3
    • 3
    • 3
    • 3
    • 3
    • 3
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    This PUMA antibody is un-conjugated
    应用范围
    • 98
    • 39
    • 39
    • 23
    • 22
    • 17
    • 16
    • 14
    • 13
    • 9
    • 5
    • 3
    • 2
    • 2
    Western Blotting (WB), Immunohistochemistry (IHC)
    特异性
    Detects the N-terminal domain of PUMA ~23 kDa. Detects ~16 kDa bands sometimes, possibly corresponding to PUMAβ.
    交叉反应
    鱼, 人, Rainbow Trout, 大鼠, Salmon
    纯化方法
    Protein A Purified
    免疫原
    N-terminal amino acids of human PUMA
    Top Product
    Discover our top product BBC3 Primary Antibody
  • 应用备注
    • WB (1:500)
    • IHC (1:100)
    • optimal dilutions for assays should be determined by the user.
    说明

    2 μg/ml of ABIN361753 was sufficient for detection of PUMA in 20 μg of human K562 cell lysate by colorimetric immunoblot analysis using Goat anti-rabbit IgG:HRP as the secondary antibody.

    限制
    仅限研究用
  • 状态
    Liquid
    浓度
    1 mg/mL
    缓冲液
    PBS, 0.02 % sodium azide, Storage buffer may change when conjugated
    储存液
    Sodium azide
    注意事项
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    储存条件
    -20 °C
    储存方法
    -20°C
  • Wakabayashi, Kosaka, Mori, Yamada: "Prolonged expression of Puma in cholinergic amacrine cells during the development of rat retina." in: The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society, Vol. 60, Issue 10, pp. 777-88, (2012) (PubMed).

  • 抗原
    PUMA (BBC3) (BCL2 Binding Component 3 (BBC3))
    别名
    PUMA (BBC3 产品)
    别名
    BBC3 antibody, Puma antibody, JFY-1 antibody, JFY1 antibody, PUMA antibody, PUMA/JFY1 antibody, BCL2 binding component 3 antibody, Bcl-2 binding component 3 antibody, BBC3 antibody, Bbc3 antibody, bbc3 antibody
    背景
    Apoptosis is related to many diseases and development. The p53 tumor-suppressor protein induces apoptosis through transcriptional activation of several genes. A novel p53 inducible pro-apoptotic gene was identified recently and designated PUMA (for p53 up-regulated modulator of apoptosis) and bbc3 (for Bcl-2 binding component 3) in human and mouse (1-3). PUMA/bbc3 is one of the pro-apoptotic Bcl-2 family members including Bax and Noxa, which are also transcriptional targets of p53. The PUMA gene encodes two BH3 domain-containing proteins termed PUMA-α and PUMA-β (1). PUMA proteins bind Bcl-2, localize to the mitochondria, and induce cytochrome c release and apoptosis in response to p53. PUMA may be a direct mediator of p53-induced apoptosis.
    基因ID
    27113
    NCBI登录号
    NP_001120712
    UniProt
    Q9BXH1
    途径
    p53 Pathway, Positive Regulation of Endopeptidase Activity
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