PUMA 抗体 (C-Term)
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- 抗原 See all PUMA (BBC3) 抗体
- PUMA (BBC3) (BCL2 Binding Component 3 (BBC3))
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抗原表位
- C-Term
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适用
- 人
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宿主
- 兔
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克隆类型
- 多克隆
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标记
- This PUMA antibody is un-conjugated
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应用范围
- Western Blotting (WB), Immunocytochemistry (ICC), Immunofluorescence (IF)
- 特异性
- Detects ~23 kDa. Detects ~16 kDa bands sometimes, possibly corresponding to PUMAβ.
- 交叉反应
- 小鸡, 人, 小鼠
- 纯化方法
- Protein A Purified
- 免疫原
- C-terminal amino acids of human PUMA
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- 应用备注
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- WB (1:1000)
- ICC/IF (1:100)
- optimal dilutions for assays should be determined by the user.
- 说明
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1 μg/ml of ABIN361747 was sufficient for detection of PUMA in 20 μg of human K562 cell lysate by colorimetric immunoblot analysis using Goat anti-rabbit IgG:HRP as the secondary antibody.
- 限制
- 仅限研究用
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- 状态
- Liquid
- 浓度
- 1 mg/mL
- 缓冲液
- PBS, 0.02 % sodium azide, Storage buffer may change when conjugated
- 储存液
- Sodium azide
- 注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 储存条件
- -20 °C
- 储存方法
- -20°C
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- 抗原
- PUMA (BBC3) (BCL2 Binding Component 3 (BBC3))
- 别名
- PUMA (BBC3 产品)
- 别名
- BBC3 antibody, Puma antibody, JFY-1 antibody, JFY1 antibody, PUMA antibody, PUMA/JFY1 antibody, BCL2 binding component 3 antibody, Bcl-2 binding component 3 antibody, BBC3 antibody, Bbc3 antibody, bbc3 antibody
- 背景
- Apoptosis is related to many diseases and development. The p53 tumor-suppressor protein induces apoptosis through transcriptional activation of several genes. A novel p53 inducible pro-apoptotic gene was identified recently and designated PUMA (for p53 up-regulated modulator of apoptosis) and bbc3 (for Bcl-2 binding component 3) in human and mouse (1-3). PUMA/bbc3 is one of the pro-apoptotic Bcl-2 family members including Bax and Noxa, which are also transcriptional targets of p53. The PUMA gene encodes two BH3 domain-containing proteins termed PUMA-α and PUMA-β (1). PUMA proteins bind Bcl-2, localize to the mitochondria, and induce cytochrome c release and apoptosis in response to p53. PUMA may be a direct mediator of p53-induced apoptosis.
- 基因ID
- 27113
- NCBI登录号
- NP_001120712
- UniProt
- Q9BXH1
- 途径
- p53 Pathway, Positive Regulation of Endopeptidase Activity
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