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HSP70 抗体 (AA 365-681)

HSP70 适用: Plasmodium falciparum WB, IF, ICC 宿主: 兔 Polyclonal unconjugated
产品编号 ABIN361730
发货至: 中国
  • 抗原 See all HSP70 抗体
    HSP70 (Heat Shock Protein 70 (HSP70))
    抗原表位
    • 27
    • 26
    • 20
    • 16
    • 16
    • 16
    • 11
    • 11
    • 7
    • 5
    • 4
    • 3
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    AA 365-681
    适用
    • 319
    • 197
    • 163
    • 71
    • 60
    • 53
    • 52
    • 52
    • 36
    • 31
    • 29
    • 29
    • 28
    • 23
    • 20
    • 18
    • 15
    • 12
    • 12
    • 12
    • 10
    • 10
    • 9
    • 9
    • 9
    • 9
    • 7
    • 6
    • 3
    • 3
    • 3
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Plasmodium falciparum
    宿主
    • 205
    • 146
    • 13
    • 11
    • 11
    克隆类型
    • 227
    • 158
    多克隆
    标记
    • 108
    • 31
    • 28
    • 26
    • 24
    • 20
    • 19
    • 18
    • 18
    • 18
    • 12
    • 5
    • 5
    • 5
    • 5
    • 5
    • 5
    • 5
    • 5
    • 4
    • 4
    • 4
    • 3
    • 3
    • 2
    • 1
    • 1
    • 1
    • 1
    This HSP70 antibody is un-conjugated
    应用范围
    • 370
    • 159
    • 140
    • 121
    • 119
    • 109
    • 98
    • 66
    • 62
    • 61
    • 41
    • 33
    • 22
    • 21
    • 21
    • 6
    • 4
    • 2
    • 2
    • 1
    • 1
    Western Blotting (WB), Immunofluorescence (IF), Immunocytochemistry (ICC)
    特异性
    Detects ~ 70 kDa. Specific to P. falciparum and does not cross-react to any protein from Human erythrocytes.
    交叉反应
    Plasmodium falciparum
    纯化方法
    Protein A Purified
    免疫原
    His-tagged and purified PfHSP70, C-terminus (AA 365-681)
    Top Product
    Discover our top product HSP70 Primary Antibody
  • 应用备注
    • WB (1:2000)
    • ICC/IF (1:50)
    • optimal dilutions for assays should be determined by the user.
    说明

    0.15 μg/ml of ABIN361729 was sufficient for detection of PfHSP70 in 20 μg of P. falciparum lysate by colorimetric immunoblot analysis using Goat anti-rabbit IgG:HRP as the secondary antibody.

    限制
    仅限研究用
  • 状态
    Liquid
    浓度
    1 mg/mL
    缓冲液
    PBS pH 7.4, 50 % glycerol, 0.09 % sodium azide, Storage buffer may change when conjugated
    储存液
    Sodium azide
    注意事项
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    储存条件
    -20 °C
    储存方法
    -20°C
  • Llorà-Batlle, Michel-Todó, Witmer, Toda, Fernández-Becerra, Baum, Cortés: "Conditional expression of PfAP2-G for controlled massive sexual conversion in Plasmodium falciparum." in: Science advances, Vol. 6, Issue 24, pp. eaaz5057, (2020) (PubMed).

    Bancells, Llorà-Batlle, Poran, Nötzel, Rovira-Graells, Elemento, Kafsack, Cortés: "Revisiting the initial steps of sexual development in the malaria parasite Plasmodium falciparum." in: Nature microbiology, Vol. 4, Issue 1, pp. 144-154, (2019) (PubMed).

    Hallée, Boddey, Cowman, Richard: "Evidence that the Plasmodium falciparum Protein Sortilin Potentially Acts as an Escorter for the Trafficking of the Rhoptry-Associated Membrane Antigen to the Rhoptries." in: mSphere, Vol. 3, Issue 1, (2018) (PubMed).

    Hallée, Richard: "Evidence that the Malaria Parasite Plasmodium falciparum Putative Rhoptry Protein 2 Localizes to the Golgi Apparatus throughout the Erythrocytic Cycle." in: PLoS ONE, Vol. 10, Issue 9, pp. e0138626, (2016) (PubMed).

    Goel, Palmkvist, Moll, Joannin, Lara, Akhouri, Moradi, Öjemalm, Westman, Angeletti, Kjellin, Lehtiö, Blixt, Ideström, Gahmberg, Storry, Hult, Olsson, von Heijne, Nilsson, Wahlgren: "RIFINs are adhesins implicated in severe Plasmodium falciparum malaria." in: Nature medicine, Vol. 21, Issue 4, pp. 314-7, (2015) (PubMed).

    Boes, Spiegel, Voepel, Edgue, Beiss, Kapelski, Fendel, Scheuermayer, Pradel, Bolscher, Behet, Dechering, Hermsen, Sauerwein, Schillberg, Reimann, Fischer: "Analysis of a Multi-component Multi-stage Malaria Vaccine Candidate-Tackling the Cocktail Challenge." in: PLoS ONE, Vol. 10, Issue 7, pp. e0131456, (2015) (PubMed).

    Sanz, Bandini, Ospina, Bernabeu, Mariño, Fernández-Becerra, Izquierdo: "Biosynthesis of GDP-fucose and other sugar nucleotides in the blood stages of Plasmodium falciparum." in: The Journal of biological chemistry, Vol. 288, Issue 23, pp. 16506-17, (2013) (PubMed).

  • 抗原
    HSP70 (Heat Shock Protein 70 (HSP70))
    别名
    HSP70 (HSP70 产品)
    别名
    APG-2 antibody, HS24/P52 antibody, HSPH2 antibody, RY antibody, hsp70 antibody, hsp70RY antibody, CG31354 antibody, HSP70 antibody, Hsp70Bb antibody, hsp70B antibody, hsp70Bb-prime antibody, DmelCG5834 antibody, CG5834 antibody, HSPA1 antibody, HSP70B' antibody, HSPA6 antibody, ARABIDOPSIS HEAT SHOCK PROTEIN 70 antibody, ATHSP70 antibody, heat shock protein 70 antibody, LOC100305036 antibody, hsc70 antibody, Hsp70 antibody, Hsp70-1 antibody, Hsp70.1 antibody, hsp68 antibody, Hsp110 antibody, irp94 antibody, HSP70-2 antibody, HSPA1B antibody, HSPA2 antibody, hsp70-5 antibody, HSP70-1 antibody, HSP70.1 antibody, HSP70.2 antibody, heat shock protein family A (Hsp70) member 4 antibody, CG5834 gene product from transcript CG5834-RA antibody, heat shock protein 70 antibody, heat shock protein family A (Hsp70) member 6 antibody, heat shock 70kDa protein 2 antibody, heat shock 70 kD protein cognate antibody, Hsp70 family chaperone antibody, Heat shock protein 70 antibody, Heat shock protein 70, putative antibody, heat shock protein 1B antibody, heat shock protein family A member 4 antibody, heat shock 70kDa protein 1A antibody, heat shock protein 1 antibody, Heat Shock Protein antibody, heat shock cognate 70-kd protein antibody, Heat shock 70 kDa protein 1A antibody, HSPA4 antibody, Hsp70Bbb antibody, HSP70 antibody, HSPA6 antibody, HSPA2 antibody, PCC7424_2419 antibody, Isop_1041 antibody, CGB_C3390W antibody, Bacsa_1698 antibody, dnaK-B antibody, LOC100305036 antibody, Hspa1b antibody, Hspa4 antibody, HSPA1A antibody, hsp1 antibody, hsp-70 antibody, hsp70 antibody, LOC108348108 antibody
    背景
    HSP70 genes encode abundant heat-inducible 70- kDa HSPs (HSP70s). In most eukaryotes HSP70 genes exist as part of a multigene family. They are found in most cellular compartments of eukaryotes including nuclei, mitochondria, chloroplasts, the endoplasmic reticulum and the cytosol, as well as in bacteria. The genes show a high degree of conservation, having at least 5O% identity (1). The N-terminal two thirds of HSP70s are more conserved than the C-terminal third. HSP70 binds ATP with high affinity and possesses a weak ATPase activity which can be stimulated by binding to unfolded proteins and synthetic peptides (2). When HSC70 (constitutively expressed) present in mammalian cells was truncated, ATP binding activity was found to reside in an N-terminal fragment of 44 kDa which lacked peptide binding capacity. Polypeptide binding ability therefore resided within the C-terminal half (3). The structure of this ATP binding domain displays multiple features of nucleotide binding proteins (4). All HSP70s, regardless of location, bind proteins, particularly unfolded ones. The molecular chaperones of the HSP70 family recognize and bind to nascent polypeptide chains as well as partially folded intermediates of proteins preventing their aggregation and misfolding. The binding of ATP triggers a critical conformational change leading to the release of the bound substrate protein (5). The universal ability of HSP70s to undergo cycles of binding to and release from hydrophobic stretches of partially unfolded proteins determines their role in a great variety of vital intracellular functions such as protein synthesis, protein folding and oligomerization and protein transport. PfHSP70-I (PF08_0054) is the major cytosolic HSP70 in Plasmodium falciparum. It is abundantly expressed in the blood stages of the parasite and is thought to constitute 1-2 % of total parasite protein. It is induced upon heat shock. It is present in the parasite in different complexes with PfHSP90 and some PfHSP40 (6, 7). Looking for more information on HSP70? Visit our new HSP70 Scientific Resource Guide at http://www.HSP70.com.
    UniProt
    P11144
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