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CAMKII gamma 抗体

CAMK2G 适用: 大鼠 WB, IHC, ELISA, IP, IF, ICC, RIA 宿主: 小鼠 Monoclonal 6G9 unconjugated
产品编号 ABIN361644
发货至: 中国
  • 抗原 See all CAMKII gamma (CAMK2G) 抗体
    CAMKII gamma (CAMK2G) (Calcium/calmodulin-Dependent Protein Kinase II gamma (CAMK2G))
    适用
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    大鼠
    宿主
    • 54
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    小鼠
    克隆类型
    • 49
    • 37
    单克隆
    标记
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    This CAMKII gamma antibody is un-conjugated
    应用范围
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    Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Immunoprecipitation (IP), Immunofluorescence (IF), Immunocytochemistry (ICC), Radioimmunoassay (RIA)
    特异性
    Detects ~50-60 kDa. Recognizes both phosphorylated and non-phosphorylated forms.
    交叉反应
    Cow, 人, 小鼠, 大鼠
    纯化方法
    Protein G Purified
    免疫原
    Partially purified rat CaMKII
    克隆位点
    6G9
    亚型
    IgG1
  • 应用备注
    • WB (1:10000)
    • IHC (1:2000)
    • ICC/IF (1:50)
    • optimal dilutions for assays should be determined by the user.
    说明

    0.1 μg/ml was sufficient for detection of CamKII in 20 μg rat brain tissue extract by colorimetric immunoblot analysis using Goat Anti-Mouse IgG:AP as the secondary.

    限制
    仅限研究用
  • 状态
    Liquid
    浓度
    1 mg/mL
    缓冲液
    PBS pH 7.4, 50 % glycerol, 0.09 % sodium azide, Storage buffer may change when conjugated
    储存液
    Sodium azide
    注意事项
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    储存条件
    -20 °C
    储存方法
    -20°C
  • Feng, Szabo, Dziak, Opas: "Cytoskeletal disassembly and cell rounding promotes adipogenesis from ES cells." in: Stem cell reviews, Vol. 6, Issue 1, pp. 74-85, (2010) (PubMed).

  • 抗原
    CAMKII gamma (CAMK2G) (Calcium/calmodulin-Dependent Protein Kinase II gamma (CAMK2G))
    别名
    CaMKII (CAMK2G 产品)
    别名
    CAMK antibody, CAMK-II antibody, CAMKG antibody, 5930429P18Rik antibody, Camkg antibody, calcium/calmodulin dependent protein kinase II gamma antibody, calcium/calmodulin-dependent protein kinase II gamma antibody, CAMK2G antibody, Camk2g antibody
    背景
    CaMKII is an important member of the calcium/calmodulin-activated protein kinase family, functioning in neural synaptic stimulation and T-cell receptor signaling (1, 2). CaMKII is expressed in many different tissues but is specifically found in the neurons of the forebrain and its mRNA is found within the dendrites and the soma of the neuron. The CaMKII that is found in the neurons consist of two subunits of 52 (termed alpha genes) and 60 kDa (beta genes). CaMKII has catalytic and regulatory domains, as well as an ATP-binding domain, and a consensus phosphorylation site (3-7). The binding of Ca2+/calmodulin to its regulatory domain releases its auto inhibitory effect and activates the kinase (8). This kinase activation results in autophosphorylation at threonine 286 (8). The threonine phosphorylation state of CaMKII can be regulated through PP1/PKA. Whereas PP1 (protein phosphatase 1) dephosphorylates phospho-CaMKII at Thr286, PKA (protein kinase A) prevents this dephosphorylation (9). Autophosphorylation also enables CaMKII to attain an enhanced affinity for NMDA receptors in postsynaptic densities (10-12).
    基因ID
    12322
    NCBI登录号
    NP_033922
    UniProt
    P11798
    途径
    WNT signaling, Interferon-gamma Pathway, Hormone Transport, Myometrial Relaxation and Contraction, Regulation of long-term Neuronal Synaptic Plasticity
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