MAP4K1 抗体 (pSer171)
-
- 抗原 See all MAP4K1 抗体
- MAP4K1 (Mitogen-Activated Protein Kinase Kinase Kinase Kinase 1 (MAP4K1))
-
抗原表位
- pSer171
-
适用
- 人
-
宿主
- 兔
-
克隆类型
- 多克隆
-
标记
- This MAP4K1 antibody is un-conjugated
-
应用范围
- Enzyme Immunoassay (EIA)
- 特异性
- This antibody detects MAP4K1 pSer171. Predicted to cross react with Mouse (100 % Antigen Homology).
- 纯化方法
- Affinity purification in a 2-step procedure with the control and phosphorylated peptides. The phospho-specific antibody is eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.
- 免疫原
- This antibody is generated from rabbits immunized with a KLH conjugated synthetic phosphopeptide corresponding to amino acid residues surrounding S171 of human MAP4K1.
- 亚型
- Ig Fraction
- Top Product
- Discover our top product MAP4K1 Primary Antibody
-
anti-Mitogen-Activated Protein Kinase Kinase Kinase Kinase 1 (MAP4K1) (pSer171) antibody
MAP4K1 适用: 人, 大鼠 WB, ELISA, IHC (p), FACS, IF (cc), IF (p), IHC (fro) 宿主: 兔 Polyclonal unconjugated
anti-Mitogen-Activated Protein Kinase Kinase Kinase Kinase 1 (MAP4K1) (Internal Region) antibodyMAP4K1 适用: 人, 小鼠, 大鼠 WB, ELISA, ICC, IF 宿主: 兔 Polyclonal unconjugated
anti-Mitogen-Activated Protein Kinase Kinase Kinase Kinase 1 (MAP4K1) (AA 278-377) antibodyMAP4K1 适用: 人 WB, ELISA 宿主: 小鼠 Monoclonal 1G6 unconjugated
anti-Mitogen-Activated Protein Kinase Kinase Kinase Kinase 1 (MAP4K1) (Tyr381) antibodyMAP4K1 适用: 人 WB 宿主: 兔 Polyclonal RB58818 unconjugated
anti-Mitogen-Activated Protein Kinase Kinase Kinase Kinase 1 (MAP4K1) (pSer171) antibodyMAP4K1 适用: 人 ELISA, DB 宿主: 兔 Polyclonal unconjugated
anti-Mitogen-Activated Protein Kinase Kinase Kinase Kinase 1 (MAP4K1) (N-Term) antibodyVerified MAP4K1 适用: 人 WB, ELISA, IHC 宿主: 山羊 Polyclonal unconjugated
anti-Mitogen-Activated Protein Kinase Kinase Kinase Kinase 1 (MAP4K1) (N-Term) antibodyMAP4K1 适用: 人 WB, IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-Mitogen-Activated Protein Kinase Kinase Kinase Kinase 1 (MAP4K1) (AA 231-330) antibodyMAP4K1 适用: 人 WB, ELISA, IHC (p), FACS, IF (cc), IF (p), IHC (fro) 宿主: 兔 Polyclonal unconjugated
anti-Mitogen-Activated Protein Kinase Kinase Kinase Kinase 1 (MAP4K1) (Internal Region) antibodyMAP4K1 适用: 人 WB, ELISA 宿主: 兔 Polyclonal unconjugated
anti-Mitogen-Activated Protein Kinase Kinase Kinase Kinase 1 (MAP4K1) (AA 346-375) antibodyMAP4K1 适用: 人 WB 宿主: 兔 Polyclonal RB11320 unconjugated
-
- 应用备注
-
ELISA: 1/1,000. Dot Blot: 1/500.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. - 限制
- 仅限研究用
-
- 状态
- Liquid
- 浓度
- 0.25 mg/mL
- 缓冲液
- PBS with 0.09 % (W/V) Sodium Azide as preservative.
- 储存液
- Sodium azide
- 注意事项
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 注意事项
- Avoid repeated freezing and thawing.
- 储存条件
- 4 °C/-20 °C
- 储存方法
- Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at-20 °C for longer.
-
- 抗原
- MAP4K1 (Mitogen-Activated Protein Kinase Kinase Kinase Kinase 1 (MAP4K1))
- 别名
- MAP4K1 (MAP4K1 产品)
- 别名
- HPK1 antibody, Hpk1 antibody, mHPK1 antibody, mitogen activated protein kinase kinase kinase kinase 1 antibody, mitogen-activated protein kinase kinase kinase kinase 1 antibody, Map4k1 antibody, MAP4K1 antibody
- 背景
- The c-Jun amino-terminal kinases (JNKs)/stress-activated protein kinases (SAPKs) play a crucial role in stress responses in mammalian cells. The mechanism underlying this pathway in the hematopoietic system is unclear, but it is a key in understanding the molecular basis of blood cell differentiation. We have cloned a novel protein kinase, termed hematopoietic progenitor kinase 1 (HPK1), that is expressed predominantly in hematopoietic cells, including early progenitor cells. HPK1 is related distantly to the p21(Cdc42/Rac1)-activated kinase (PAK) and yeast STE20 implicated in the mitogen-activated protein kinase (MAPK) cascade. Expression of HPK1 activates JNK1 specifically, and it elevates strongly AP-1-mediated transcriptional activity in vivo. HPK1 binds and phosphorylates MEKK1 directly, whereas JNK1 activation by HPK1 is inhibited by a dominant-negative MEKK1 or MKK4/SEK mutant. Interestingly, unlike PAK65, HPK1 does not contain the small GTPase Rac1/Cdc42-binding domain and does not bind to either Rac1 or Cdc42, suggesting that HPK1. activation is Rac1/Cdc42-independent. These results indicate that HPK1 is a novel functional activator of the JNK/SAPK signaling pathway.Synonyms: HPK1, Hematopoietic progenitor kinase, MAPK/ERK kinase kinase kinase 1, MEKKK 1, Mitogen-activated protein kinase kinase kinase kinase 1
- 分子量
- 91166 Da
- 基因ID
- 11184, 9606
- UniProt
- Q92918
- 途径
- TCR Signaling, Signaling of Hepatocyte Growth Factor Receptor
-
