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V5 Epitope 标记 抗体

ABIN356537 产品详细信息, 供应商: Log in to see
抗原
适用
标记
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宿主

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克隆类型
多克隆
标记
非结合性
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应用范围
Enzyme Immunoassay (EIA), Western Blotting (WB)
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Supplier
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免疫原 KLH conjugated synthetic peptide encoding the V5 tag (GKPIPNPLLGLDST) was used as antigen.
序列 GKPIPNPLLG LDST
亚型 Ig
纯化方法 Protein G Chromatography, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.
别名 Pk (V5) Epitope Tag (GKPIPNPLLGLDST)
物质类 Tag
背景 Epitope tags consisting of short sequences recognized by well-characterizated antibodies have been widely used in the study of protein expression in various systems. The V5 epitope tag is is composed of a 14 residue peptide, GKPIPNPLLGLDST, derived from a small epitope (Pk) present on the P and V proteins of the paramyxovirus of simian virus 5 (SV5). This polyclonal antibody provides a simple solution to detect the expression of a V5-tagged protein in cells.
研究领域 Tags/Labels
应用备注 ELISA: 1/1,000. Western Blot: 1/100-1/500.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
限制 仅限研究用
状态 Liquid
浓度 0.25 mg/mL
缓冲液 PBS containing 0.09 % (w/v) Sodium Azide as preservative.
储存液 Sodium azide
注意事项 This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
注意事项 Avoid repeated freezing and thawing.
储存条件 4 °C/-20 °C
储存方法 Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at-20 °C for longer.
厂商提供的图像
 image for anti-V5 Epitope 标记 抗体 (ABIN356537) anti-V5 Epitope Tag antibody
 image for anti-V5 Epitope 标记 抗体 (ABIN356537) anti-V5 Epitope Tag antibody (Image 2)
背景 Dunn, ODowd, Randall: "Fine mapping of the binding sites of monoclonal antibodies raised against the Pk tag." in: Journal of immunological methods, Vol. 224, Issue 1-2, pp. 141-50, 1999 (PubMed).