GFP 抗体 (Green Fluorescent Protein)

Details for Product anti-GFP Antibody No. ABIN356346, 供应商: Log in to see
抗原
  • green fluorescent protein
  • gfp
适用
Aequorea victoria
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23
16
5
5
2
2
1
1
宿主
小鼠
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171
83
41
16
9
9
2
克隆类型 (克隆位点)
单克隆 ()
标记
This GFP antibody is un-conjugated
33
31
28
14
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11
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2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
应用范围
Enzyme Immunoassay (EIA), Immunofluorescence (IF), Western Blotting (WB)
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203
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136
119
87
73
24
15
13
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8
8
6
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3
3
3
2
2
1
1
1
1
选项
Supplier
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Supplier Product No.
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免疫原 Purified His-tagged GFP protein was used to produced this monoclonal antibody.
克隆位点 168AT1211
亚型 IgG1
产品特性 Synonyms: Green fluorescent protein, GFP-Tag
纯化方法 Protein G Chromatography eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.
别名 GFP
物质类 Tag
背景 Green fluorescent protein (GFP), originally isolated from the jellyfish Aequorea victoria, is one of the best visual reporters for monitoring gene expression in vivo and in situ. GFP is a also convenient marker for use in flow cytometry because it eliminates the need to incubate with asecondary reagent (such as dyes or antibodies) for detection. However, anti-GFP antibody is also widely used for co-immunoprecipitation, co-localization or western blotting for the confirmation of specificity when a GFP fusion protein is expressed in cells. This monoclonal antibody provides a simple solution to detect the expression of a GFP-tagged protein in cells. Because of its ability to spontaneously generate its own fluorophore, the green fluorescent protein (GFP) from the jellyfish Aequorea victoria is used extensively as a fluorescent marker in molecular and cell biology. The yellow fluorescent proteins (YFPs) have the longest wavelength emissions of all GFP variants examined to date. This shift in the spectrum is the result of a T203Y substitution (single-letter amino acid code), a mutation rationally designed on the basis of the X-ray structure of GFP S65T. Anti-GFP monoclonal antibody can detect both GFP and YFP but not BFP (Blue fluorescent protein) by western blotting.Synonyms: GFP-Tag, Green fluorescent protein
UniProt P42212
研究领域 Tags/Labels
应用备注 ELISA: 1/3,000. Western blotting: 1/2,000. Immunohistochemistry: 1/50-1/100. Immunofluorescence.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
限制 仅限研究用
状态 Liquid
浓度 0.25 mg/mL
缓冲液 PBS containing 0.09 % (w/v) Sodium Azide as preservative.
储存液 Sodium azide
注意事项 This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
储存条件 4 °C/-20 °C
储存方法 Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer. Avoid repeated freezing and thawing.
Shelf life: one year from despatch.
有效期 12 months
厂商提供的图像
Western Blotting (WB) image for anti-Green Fluorescent Protein (GFP) antibody (ABIN356346) anti-Green Fluorescent Protein (GFP) antibody
Immunofluorescence (IF) image for anti-Green Fluorescent Protein (GFP) antibody (ABIN356346) anti-Green Fluorescent Protein (GFP) antibody (Image 2)
有引用在: Clark, Jovov, Rooj, Fuller, Benos: "Proteolytic cleavage of human acid-sensing ion channel 1 by the serine protease matriptase." in: The Journal of biological chemistry, Vol. 285, Issue 35, pp. 27130-43, 2010 (PubMed).

Maier, Ogihara, Trace, Tersey, Robbins, Chakrabarti, Nunemaker, Stull, Taylor, Thompson, Dondero, Lewis, Dinarello, Nadler, Mirmira: "The unique hypusine modification of eIF5A promotes islet beta cell inflammation and dysfunction in mice." in: The Journal of clinical investigation, Vol. 120, Issue 6, pp. 2156-70, 2010 (PubMed).

Kim, Nadal, Clemens, Baron, Jung, Misumi, Rudy, Hoffman: "Kv4 accessory protein DPPX (DPP6) is a critical regulator of membrane excitability in hippocampal CA1 pyramidal neurons." in: Journal of neurophysiology, Vol. 100, Issue 4, pp. 1835-47, 2008 (PubMed).

Witola, Pessi, El Bissati, Reynolds, Mamoun: "Localization of the phosphoethanolamine methyltransferase of the human malaria parasite Plasmodium falciparum to the Golgi apparatus." in: The Journal of biological chemistry, Vol. 281, Issue 30, pp. 21305-11, 2006 (PubMed).

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