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Keratin Acidic (AE1) 抗体

AE1 适用: 人, 大鼠, 小鸡, 小鼠, 兔, 猴, Cow, Pig IF, IHC (p), IHC (fro) 宿主: 小鼠 Monoclonal AE1 unconjugated
产品编号 ABIN356303
发货至: 中国
  • 抗原 See all Keratin Acidic (AE1) 抗体
    Keratin Acidic (AE1)
    适用
    • 32
    • 11
    • 9
    • 9
    • 9
    • 7
    • 6
    • 5
    • 4
    • 3
    • 2
    • 2
    人, 大鼠, 小鸡, 小鼠, 兔, 猴, Cow, Pig
    宿主
    • 18
    • 11
    • 3
    小鼠
    克隆类型
    • 32
    单克隆
    标记
    • 15
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 1
    非结合性
    应用范围
    • 24
    • 24
    • 24
    • 20
    • 12
    • 3
    • 3
    • 2
    Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))
    特异性
    This antibody recognizes low molecular weight cytokeratins (CK 10 (56.5), CK14 (50), CK15 (50), CK16 (48) and CK19(40) of the acidic family.
    Cellular Localization: Cytoplasmic.
    产品特性
    Synonyms: LMW Cytokeratins, Cytokeratin type I, Keratin type I
    纯化方法
    Protein A Chromatography.
    免疫原
    Human epithelial keratin. Remarks: Molecular weight of antigen: (CK 10 (56. 5), CK14 (50), CK15 (50), CK16 (48) andCK19 (40).
    克隆位点
    AE1
    亚型
    IgG1
    Top Product
    Discover our top product AE1 Primary Antibody
  • 应用备注
    Suitable for Immunohistochemistry and Immunocytochemistry (Frozen or Formalin-FixedParaffin-Embedded (FFPE) tissue sections and cell smears). For IHC dilute concentared antibody at 1/50-1/100, use streptavidinapprox. biotin system orpolymer system, incubate 30 minutes at room temperature. FFPE tissue section requires antigen retrieval (boiling tissue in 10 mM citrate, pH 6.0 for10-15 minutes followed by cooling for 10-15 minutes). Recommended Positive Control: Human skin, squamous cell Ca.
    Other applications not tested.
    Optimal dilutions are dependent on conditions and should be determined by the user.
    限制
    仅限研究用
  • 状态
    Liquid
    浓度
    0.2 mg/mL
    缓冲液
    PBS, pH 7.4 containing 1 % BSA as stabilizer and 0.05 % Sodium Azide as preservative.
    储存液
    Sodium azide
    注意事项
    This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    储存条件
    4 °C/-20 °C
    储存方法
    Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer. Avoid repeated freezing and thawing.
    Shelf life: One year from despatch.
    有效期
    12 months
  • 抗原
    Keratin Acidic (AE1)
    Abstract
    AE1 产品
    背景
    Cytokeratins are intermediate filament keratins found in the intracytoplasmic cytoskeleton of epithelial tissue There are two types of Cytokeratins: the low weight, acidic type I cytokeratins and the high weight, basic or neutral type II. Cytokeratins are usually found in pairs comprising a type I Cytokeratin and a type II cytokeratin. The high molecular weight cytokeratins, which are the basic or neutral cytokeratins, comprise subtypes CK1, CK2, CK3, CK4, CK5, CK6, CK7, CK8 and CK9. The low molecular weight cytokeratins, which are the acidic cytokeratins, comprise subtypes CK10, CK12, CK 13, CK14, CK16, CK17, CK18, CK19 and CK20.Synonyms: Cytokeratin type I, Keratin type I, LMW Cytokeratins
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