A synthetic peptide from the c-terminal region of mouse VPS29 conjugated to an immunogenic carrier protein was used as the antigen. The peptide is homologous in rat, human, chicken, zebra fish and xenopus.
VPS29
适用: 小鼠
IF (p), IF (cc)
宿主: 兔
Polyclonal
Alexa Fluor 350
应用备注
IHC, WB. A concentration of 10-50 μg,ml is recommended. The optimal concentration should be determined by the end user. Not tested in other applications.
限制
仅限研究用
状态
Lyophilized
溶解方式
Reconstitute in 500 μL of sterile water. Centrifuge to remove any insoluble material.
注意事项
Avoid freeze and thaw cycles.
储存条件
4 °C/-20 °C
储存方法
Maintain the lyophilised/reconstituted antibodies frozen at -20°C for long term storage and refrigerated at 2-8°C for a shorter term. When reconstituting, glycerol (1:1) may be added for an additional stability. Avoid freeze and thaw cycles.
FUNCTION: Essential component of the retromer complex, a complex required to retrieve lysosomal enzyme receptors (IGF2R and M6PR) from endosomes to the trans-Golgi network. Also required to regulate transcytosis of the polymeric immunoglobulin receptor (pIgR-pIgA). Has low protein phosphatase activity towards a serine-phosphorylated peptide derived from IGF2R (in vitro). Has no activity towards p-nitrophenylphosphate, p-nitrophenylphosphorylcholine or phosphatidylinositlphosphates (in vitro). CATALYTIC ACTIVITY: O-phospho-L(or D)-serine + H2O = L(or D)-serine + phosphate. COFACTOR: Binds 2 zinc ions per subunit. SUBCELLULAR LOCATION: Cytoplasm. Membrane, Peripheral membrane protein. Endosome membrane, Peripheral membrane protein.,Vesicle Protein Sorting,Vacuolar protein sorting-associated protein 29, Vesicle protein sorting 29, VPS29