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TrkA, B, C (pTyr683), (pTyr684) 抗体

适用: 大鼠 WB, IHC 宿主: 兔 Polyclonal unconjugated
产品编号 ABIN351067
发货至: 中国
  • 抗原
    TrkA, B, C
    抗原表位
    pTyr683, pTyr684
    适用
    • 38
    • 38
    • 24
    大鼠
    宿主
    • 39
    克隆类型
    • 30
    • 9
    多克隆
    标记
    • 4
    • 3
    • 3
    • 3
    • 3
    • 3
    • 3
    • 3
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    非结合性
    应用范围
    • 32
    • 27
    • 26
    • 7
    • 6
    • 6
    • 2
    Western Blotting (WB), Immunohistochemistry (IHC)
    特异性
    Specific for TrkA, TrkB, TrkC.
    交叉反应
    人, 小鼠, 大鼠
    交叉反应 (详细)
    Other species not yet tested.
    纯化方法
    IgG
    免疫原
    A synthetic peptide to phospho Y683 & Y684 of rat TrkA (and the corresponding phosphorylated sequences in TrkB and TrkC) conjugated to an immunogenic carrier protein was used as the antigen. The antigen is homologous in mouse, human, chicken and zebrafish. Additionally, the antigen shares ~90% identity with the corresponding phosphorylated sequences in TrkB and TrkC.
    亚型
    IgG
  • 应用备注
    IHC, WB. A concentration of 10-50 μg,ml is recommended. The optimal concentration should be determined by the end user. Not yet tested in other applications. This antibody recognises also the corresponding phosphorylated sequences in TrkB,TrkC.
    限制
    仅限研究用
  • 状态
    Lyophilized
    溶解方式
    Reconstitute in 500 μL of sterile water. Centrifuge to remove any insoluble material.
    注意事项
    Avoid freeze and thaw cycles.
    储存条件
    4 °C/-20 °C
    储存方法
    Maintain the lyophilised/reconstituted antibodies frozen at -20°C for long term storage and refrigerated at 2-8°C for a shorter term. When reconstituting, glycerol (1:1) may be added for an additional stability. Avoid freeze and thaw cycles.
    有效期
    12 months
  • 抗原
    TrkA, B, C
    物质类
    Tag
    背景
    FUNCTION: Required for high-affinity binding to nerve growth factor (NGF), neurotrophin-3 and neurotrophin-4/5 but not brain-derived neurotrophic factor (BDNF). Known substrates for the Trk receptors are SHC1, PI 3-kinase, and PLC-gamma-1. Has a crucial role in the development and function of the nociceptive reception system as well as establishment of thermal regulation via sweating. Activates ERK1 by either SHC1- or PLC-gamma-1-dependent signaling pathway. CATALYTIC ACTIVITY: ATP + a [protein]-L-tyrosine = ADP + a [protein]-L-tyrosine phosphate. SUBUNIT: Exists in a dynamic equilibrium between monomeric (low affinity) and dimeric (high affinity) structures. Binds SH2B2. Interacts with SQSTM1 which bridges NTRK1 to NGFR. Interacts with NGFR and ARMS. Can form a ternary complex with NGFR and ARMS and this complex is affected by the expression levels of ARMS. An increase in ARMS expression leads to a decreased association of NGFR and NTRK1. SUBCELLULAR LOCATION: Cell membrane, Single-pass type I membrane protein. Note: Endocytosed to the endosomes upon treatment of cells with NGF. TISSUE SPECIFICITY: Isoform Trka-II is primarily expressed in neuronal cells, isoform Trka-I is found in non-neuronal tissues.,Neurotrophic Receptors,High affinity nerve growth factor receptor, NTRK1, p140-TrkA, Trk-A, Neurotrophic tyrosine kinase receptor type 1, Slow nerve growth factor receptor
    UniProt
    P35739
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