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GFP 抗体

GFP 适用: 所有种类 WB, IHC 宿主: 兔 Polyclonal unconjugated
产品编号 ABIN350304
发货至: 中国
  • 抗原 See all GFP 抗体
    GFP (Green Fluorescent Protein (GFP))
    适用
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    所有种类
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    克隆类型
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    多克隆
    标记
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    This GFP antibody is un-conjugated
    应用范围
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    Western Blotting (WB), Immunohistochemistry (IHC)
    特异性
    Specific for EGFP/GFP/Venus
    免疫原
    Synthetic peptides (a pentamer) from EGFP/GFP/Venus conjugated to an immunogenic carrier protein was used as the antigen.
    亚型
    IgG
    Top Product
    Discover our top product GFP Primary Antibody
  • 应用备注
    A working concentration of 5-50 µg/ml is recommended.
    The optimal concentration should be determined by the end user.
    限制
    仅限研究用
  • 状态
    Lyophilized
    溶解方式
    Reconstitute in 100 µL of sterile water. Centrifuge to remove any insoluble material.
    注意事项
    Avoid freeze and thaw cycles.
    储存条件
    4 °C/-20 °C
    储存方法
    Maintain the lyophilised/reconstituted antibodies frozen at -20°C for long term storage and refrigerated at 2-8°C for a shorter term. When reconstituting, glycerol (1:1) may be added for an additional stability. Avoid freeze and thaw cycles.
    有效期
    12 months
  • 抗原
    GFP (Green Fluorescent Protein (GFP))
    Abstract
    GFP 产品
    别名
    green fluorescent protein antibody, gfp antibody
    物质类
    Viral Protein
    背景
    GFP is a  naturally fluorescent protein, discovered by chance in the 1960s by Shimomura et al. GFP makes green light out of aequorin’s blue light. The chromophore is a modified Ser-Tyr-Gly sequence. GFP can emit light once this short tripeptide has adopted a cyclic conformation with the help of a oxygen. BIOPHYSICOCHEMICAL PROPERTIES: Excitation max (nm): 488, Emission max (nm): 509, Extinction coefficient (Cm-1M-1): 61000. Fluorescent proteins have become a useful and ubiquitous tool for making chimeric proteins, where they function as a fluorescent protein tag. Typically they tolerate N- and C-terminal fusion to a broad variety of proteins. They have been expressed in most known cell types and are used as a noninvasive fluorescent marker in living cells and organisms. They enable a wide range of applications where they have functioned as a cell lineage tracer, reporter of gene expression, or as a measure of protein-protein interactions. Also known as: (Enhanced) Green Fluorescence Protein.
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