A synthetic peptide from the phospho Y140 & S156 of human Cofilin-1 (Cofilin, non-muscle isoform) conjugated to blue carrier protein was used as the antigen. The antigen is homologous in rat, mouse and monkey.
IHC, WB. A concentration of 10-50 μg,ml is recommended. The optimal concentration should be determined by the end user. This antibody may also recognice the unphosphorylated form. Not tested in other applications.
限制
仅限研究用
状态
Lyophilized
溶解方式
Reconstitute in 500 μL of sterile water. Centrifuge to remove any insoluble material.
注意事项
Avoid freeze and thaw cycles.
储存条件
4 °C/-20 °C
储存方法
Maintain the lyophilised/reconstituted antibodies frozen at -20°C for long term storage and refrigerated at 2-8°C for a shorter term. When reconstituting, glycerol (1:1) may be added for an additional stability. Avoid freeze and thaw cycles.
FUNCTION: Controls reversibly actin polymerization and depolymerization in a pH -sensitive manner. It has the ability to bind G- and F-actin in a 1:1 ratio of cofilin to actin. It is the major component of intranuclear and cytoplasmic actin rods. SUBCELLULAR LOCATION: Nucleus matrix. Cytoplasm, cytoskeleton. Note: Almost completely in nucleus in cells exposed to heat shock or 10 % dimethyl sulfoxide. TISSUE SPECIFICITY: Widely distributed in various tissues.,Growth & Development,Cofilin, non-muscle isoform