Occludin 抗体 (C-Term)
(1 reference)-
- 抗原 See all Occludin (OCLN) 抗体
- Occludin (OCLN)
-
抗原表位
- C-Term
-
适用
- 人, 小鼠, 大鼠, 犬
-
宿主
- 小鼠
-
克隆类型
- 单克隆
-
标记
- This Occludin antibody is un-conjugated
-
应用范围
- Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
- 品牌
- IHC-plus™
- 特异性
- Recognizes mammalian Occludin Protein. Species cross-reactivity: human, mouse, rat and canine.
- 纯化方法
- Protein A purified
- 免疫原
-
GST fusion protein consisting of the C-terminal region (~150aa) of human occludin.
Type of Immunogen: Fusion protein - 克隆位点
- OC-3F10
- 亚型
- IgG1 kappa
- Top Product
- Discover our top product OCLN Primary Antibody
-
anti-Occludin (OCLN) (AA 431-522) antibody
OCLN 适用: 人, 小鼠, Pig WB, ELISA, FACS, IF (cc), IF (p), IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-Occludin (OCLN) (C-Term) antibodyOCLN 适用: 人 WB, IHC (p), IHC (fro) 宿主: 山羊 Polyclonal unconjugated
anti-Occludin (OCLN) (AA 282-415) antibodyOCLN 适用: 人 WB, IHC (p) 宿主: 小鼠 Monoclonal CL1555 unconjugated
anti-Occludin (OCLN) (AA 423-522) antibodyOCLN 适用: 人 WB, ELISA, IP, IF 宿主: 小鼠 Monoclonal 1G7 unconjugated
anti-Occludin (OCLN) (AA 1-522) antibodyOCLN 适用: 人 WB, PLA 宿主: 兔 Polyclonal unconjugated
anti-Occludin (OCLN) (AA 1-522) antibodyOCLN 适用: 人 WB, IP 宿主: 兔 Polyclonal unconjugated
anti-Occludin (OCLN) (AA 282-415) antibodyOCLN 适用: 人 WB, IHC (f) 宿主: 小鼠 Monoclonal OCLN-2181 unconjugated
anti-Occludin (OCLN) (AA 285-430) antibodyOCLN 适用: 人 WB, ELISA, IHC, IF 宿主: 兔 Polyclonal unconjugated
anti-Occludin (OCLN) (AA 350-450) antibodyOCLN 适用: 人 WB 宿主: 兔 Polyclonal unconjugated
anti-Occludin (OCLN) (AA 282-415) antibodyOCLN 适用: 人 IHC 宿主: 小鼠 Monoclonal MSVA-415M unconjugated
-
- 应用备注
-
Approved: ELISA (0.1 - 1 μg/mL), IF (2 - 3 μg/mL), IHC, IHC-P (10 μg/mL), WB (0.1 - 1 μg/mL)
Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 10 μg/mL. Positive control: T84 cell line (human intestinal epithelium), MDCK cells (canine kidney), rat liver and Caco-2 cells (human colon adenocarcinoma). Does not appear to detect the most highly phosphorylated form of the occludin protein when tested on Caco-2 cells. - 说明
-
Target Species of Antibody: Human
- 限制
- 仅限研究用
-
- 状态
- Liquid
- 浓度
- Lot specific
- 缓冲液
- PBS, pH 7.4, 0.09 % sodium azide.
- 储存液
- Sodium azide
- 注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 注意事项
- Avoid repeat freeze-thaw cycles.
- 储存条件
- 4 °C,-20 °C
- 储存方法
- Short term: 4°C. Long term: Store at -20°C. Avoid freeze-thaw cycles.
-
-
: "Blood-brain barrier breakdown after embolic stroke in rats occurs without ultrastructural evidence for disrupting tight junctions." in: PLoS ONE, Vol. 8, Issue 2, pp. e56419, (2013) (PubMed).
-
: "Blood-brain barrier breakdown after embolic stroke in rats occurs without ultrastructural evidence for disrupting tight junctions." in: PLoS ONE, Vol. 8, Issue 2, pp. e56419, (2013) (PubMed).
-
- 抗原
- Occludin (OCLN)
- 别名
- OCLN / Occludin (OCLN 产品)
- 别名
- AI503564 antibody, Ocl antibody, ocln antibody, oclnb antibody, tpmt antibody, OCLN antibody, wu:fd23h10 antibody, wu:fi13c01 antibody, zgc:113992 antibody, zgc:56359 antibody, BLCPMG antibody, occludin antibody, occludin S homeolog antibody, occludin a antibody, Ocln antibody, OCLN antibody, ocln.S antibody, ocln antibody, oclna antibody
- 背景
-
Name/Gene ID: OCLN
Synonyms: OCLN, Occludin, BLCPMG - 基因ID
- 100506658
- UniProt
- Q16625
- 途径
- Cell-Cell Junction Organization, Hepatitis C
-
