电话:
400-7060-959
传真:
+86 10 56315212-8813
电子邮件:
orders@antibodies-online.cn

NEFH 抗体

NEFH 适用: 人, 大鼠, 小鼠, 小鸡, Cow, 豚鼠, 兔, 犬, 绵羊, 非洲爪蟾, 猴, Hamster WB, IHC, IHC (p), IHC (fro) 宿主: 小鼠 Monoclonal RNF402 unconjugated
产品编号 ABIN335369
发货至: 中国
  • 抗原 See all NEFH 抗体
    NEFH (Neurofilament, Heavy Polypeptide (NEFH))
    适用
    • 108
    • 81
    • 63
    • 47
    • 28
    • 27
    • 11
    • 11
    • 8
    • 8
    • 8
    • 6
    • 6
    • 4
    • 3
    • 3
    • 2
    • 1
    人, 大鼠, 小鼠, 小鸡, Cow, 豚鼠, 兔, 犬, 绵羊, 非洲爪蟾, 猴, Hamster
    宿主
    • 66
    • 56
    • 10
    小鼠
    克隆类型
    • 82
    • 50
    单克隆
    标记
    • 89
    • 5
    • 3
    • 3
    • 3
    • 3
    • 3
    • 3
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    This NEFH antibody is un-conjugated
    应用范围
    • 89
    • 43
    • 37
    • 29
    • 26
    • 24
    • 23
    • 16
    • 14
    • 13
    • 13
    • 10
    • 5
    • 2
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    Western Blotting (WB), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))
    特异性
    Human, rat, rabbit, dog, hamster, chicken, mouse, cow, monkey, sheep, guinea pig and Xenopus.
    纯化方法
    Purified
    免疫原
    RNF402 is a mouse monoclonal IgM antibody derived by fusion of SP2/0-Ag14 mouse myeloma cells with spleen cells from a mouse immunized with a neurofilament preparation of calf brain tissue.
    克隆位点
    RNF402
    亚型
    IgM
    Top Product
    Discover our top product NEFH Primary Antibody
  • 应用备注
    RNF402 reacts with both the phosphorylated and non-phosphorylated isoform of the 200 kD neurofilament protein. RNF402 is suitable for immunoblotting and immunohistochemistry on frozen and paraffin-embedded tissues. Optimal antibody dilution should be determined by titration, recommended range is 1:10 - 1:100 for immunohistochemistry with avidin-biotinylated horseradish peroxidase complex (ABC) as detection reagent, and 1:25 - 1:250 for immunoblotting applications.
    限制
    仅限研究用
  • 储存条件
    4 °C
  • Bauwens, De Groot, Ramaekers, Veldman, Huizing: "Expression of intermediate filament proteins in the adult human vestibular labyrinth." in: The Annals of otology, rhinology, and laryngology, Vol. 101, Issue 6, pp. 479-86, (1992) (PubMed).

    Kuijpers, Tonnaer, Peters, Ramaekers: "Expression of intermediate filament proteins in the mature inner ear of the rat and guinea pig." in: Hearing research, Vol. 52, Issue 1, pp. 133-46, (1991) (PubMed).

  • 抗原
    NEFH (Neurofilament, Heavy Polypeptide (NEFH))
    别名
    Neurofilament 200 kDa (NEFH 产品)
    别名
    NF-H antibody, NF200 antibody, Nfh antibody, mKIAA0845 antibody, NFH antibody, NEFH antibody, neurofilament heavy polypeptide antibody, neurofilament, heavy polypeptide antibody, neurofilament heavy antibody, MCYG_06231 antibody, Nefh antibody, NEFH antibody
    背景
    Like most other intermediate filament proteins (IFPs), the expression of the different neuronal IFPs is both tissue-specific and developmentally regulated. The neurofilament (NF) triplet proteins (70, 160, and 200 kDa) occur in both the central and peripheral nervous system and are normally restricted to neurons. The 70 kDa NF-protein can self-assemble into a filamentous structure, whereas the 160 kDa and 200 kDa NF-proteins require the presence of the 70 kDa NF-protein to co-assemble. All three NF proteins can be detected by immunohistochemical methods at day 9 or 10 after gestation in the mouse embryo. Although IFPs of the neurofilament type are normally restricted to neurons, there are reports on their expression in non-neuronal cells as well. For example, in heart conduction myocytes NF proteins are expressed together with desmin. In tumorpathology ganglioneuroblastomas and some of the other neuroblastomas are strongly positive with the neurofilament antisera. Also, some neuro-endocrine malignancies may show NF positivity. In cell cultures of neural tissues the neurofilament antibodies can monitor in vitro differentiation.
You are here:
客服