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Method employed by authors: Immunohistochemistry (IHC) Further details: When immunohistochemistry was performed on sections of nNOS-Cre-tdTomato mice, resectioned slices (50 μm) were incubated with a primary antibody raised against nNOS and tdTomato (1:500 Rat RFP 5F8) overnight at 4°C.
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