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HCST 抗体 (Hematopoietic Cell Signal Transducer) (C-Term)

Details for Product anti-HCST Antibody No. ABIN334384, 供应商: Log in to see
抗原
  • HCST
  • DAP10
  • KAP10
  • PIK3AP
  • Dap10
  • hematopoietic cell signal transducer
  • HCST
  • hcst
  • Hcst
抗原表位
C-Term
12
2
1
1
1
适用

30
1
1
1
宿主
山羊
15
15
克隆类型
多克隆
标记
This HCST antibody is un-conjugated
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
实验类型
Peptide ELISA
应用范围
ELISA, Western Blotting (WB)
15
13
12
3
1
Supplier
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免疫原 Peptide with sequence C-RSPAQEDGKVYIN, from the C Terminus of the protein sequence according to NP_055081.1, NP_001007470.1.
Blocking Peptide Blocking peptide for this product available: ABIN369830
特异性 This antibody is expected to recognise isoform 1 (NP_055081.1) but it may recognize isoform 2 lacking residue E81 (NP_001007470.1).
交叉反应 小鼠, 大鼠
纯化方法 Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
别名 DAP10 / HCST (HCST Antibody 摘要)
背景 Synonyms: HCST, DAP10, hematopoietic cell signal transducer, KAP10, PIK3AP, DNAX-activation protein 10, phosphoinositide-3-kinase adaptor protein
基因ID 10870, 23900, 474146
NCBI登录号 NP_001007470, NP_055081
应用备注 ELISA: antibody detection limit dilution 1:128000.
Western blot: Approx 40 kDa band observed in human spleen lysates (calculated MW of 9.5 kDa according to NP_055081.1). The observed molecular weight corresponds to earlier findings in literature with different antibodies (Wu et al, Science. 1999 Jul 30,285(5428):730-2., PMID: 10426994). Recommended concentration: 0.2-0.6 µg/mL. An additional band of unknown identity was also consistently observed at 100 kDa. This band was successfully blocked by incubation with the immunising peptide.
限制 仅限研究用
状态 Liquid
缓冲液 Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
储存液 Sodium azide
注意事项 This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
注意事项 Minimize freezing and thawing.
储存条件 -20 °C
储存方法 Aliquot and store at -20 °C.
厂商提供的图像
 image for anti-HCST 抗体 (Hematopoietic Cell Signal Transducer) (C-Term) (ABIN334384) ABIN334384 (0.2µg/ml) staining of human spleen lysate (35µg protein in RIPA buffer). ...
背景 Upshaw, Arneson, Schoon, Dick, Billadeau, Leibson: "NKG2D-mediated signaling requires a DAP10-bound Grb2-Vav1 intermediate and phosphatidylinositol-3-kinase in human natural killer cells." in: Nature immunology, Vol. 7, Issue 5, pp. 524-32, 2006 (PubMed).