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CD31 抗体 (FITC)

PECAM1 适用: 大鼠 FACS 宿主: 小鼠 Monoclonal TLD-3A12 FITC
产品编号 ABIN320312
发货至: 中国
  • 抗原 See all CD31 (PECAM1) 抗体
    CD31 (PECAM1) (Platelet/endothelial Cell Adhesion Molecule (PECAM1))
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    This CD31 antibody is conjugated to FITC
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    Flow Cytometry (FACS)
    特异性
    This antibody recognises PECAM-1 (CD31), a type 1 transmembrane protein expressed primarily on endothelial cells, platelets and leucocytes. Clone TLD-3A12 has been shown to partially block the proliferative response of antigen-specific CD4+ T cells to antigen-presenting cells and relevant antigen (Stevenson, K.S. et al. 2009). We recommend SM272LE for use in functional studies. This clone has been reported as suitable for use on formalin-fixed paraffin-embedded sections pre-treated with 0.2M boric acid, pH 7.0. (Wilson et al. 2007). Mouse anti Rat CD31, clone TLD-3A12 has been shown to be cross-reactive with endothelial cells derived from rhesus macaque (Maclean et al. 2001).
    交叉反应 (详细)
    Species reactivity (expected):Rhesus Monkey.
    Species reactivity (tested):Rat.
    纯化方法
    Affinity Chromatography on Protein G.
    免疫原
    Activated, Lewis rat derived microglial cells. Spleen cells from immunised BALB/c mouse were fused with cells of the mouse SP2 myeloma cell line.
    克隆位点
    TLD-3A12
    亚型
    IgG1
    Top Product
    Discover our top product PECAM1 Primary Antibody
  • 应用备注
    Flow Cytometry: Use 10 μL of neat antibody to label 10^6 cells in 100 μL.
    Other applications not tested.
    Optimal dilutions are dependent on conditions and should be determined by the user.
    限制
    仅限研究用
  • 浓度
    0.1 mg/mL
    缓冲液
    PBS, 0.09 % Sodium Azide, 1 % BSA
    储存液
    Sodium azide
    注意事项
    This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    注意事项
    Avoid repeated freezing and thawing. This product is photosensitive and should be protected from light
    储存条件
    4 °C/-20 °C
    储存方法
    Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.
  • 抗原
    CD31 (PECAM1) (Platelet/endothelial Cell Adhesion Molecule (PECAM1))
    别名
    CD31 / PECAM1 (PECAM1 产品)
    背景
    CD31, also known as platelet endothelial cell adhesion molecule 1 (PECAM1), is a type I integral membrane glycoprotein and a member of the immunoglobulin superfamily of cell surface receptors. It is constitutively expressed on the surface of endothelial cells, and concentrated at the junction between them. It is also weakly expressed on many peripheral lymphoid cells and platelets. CD31 has been used to measure angiogenesis in association with tumor recurrence. Other studies have also indicated that CD31 and CD34 can be used as markers for myeloid progenitor cells and recognize different subsets of myeloid leukemia infiltrates (granular sarcomas).Synonyms: End °CAM, GPIIA', PECAM-1, Platelet endothelial cell adhesion molecule
    基因ID
    29583
    NCBI登录号
    NP_113779
    UniProt
    Q3SWT0
    途径
    Regulation of Actin Filament Polymerization
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