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HA-Tag 抗体

WB, EIA, IHC (p) 宿主: 兔 Polyclonal unconjugated
产品编号 ABIN318728
发货至: 中国
  • 抗原 See all HA-Tag products
    HA-Tag
    适用
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    宿主
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    克隆类型
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    This HA-Tag antibody is un-conjugated
    应用范围
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    Western Blotting (WB), Enzyme Immunoassay (EIA), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
    序列
    YPYDVPDYA
    纯化方法
    Purified
    免疫原
    Keyhole limpet hemocyanin conjugated epitope tag peptide (114-122) from haemagglutinin influenza. A cysteine residue was used to facilitate coupling at the C-terminal end.
    亚型
    IgG
  • 应用备注
    Immunohistochmemistry on paraffin sections (Requires antigen retrieval using heattreatment prior to staining of paraffin embedded sections. Citrate buffer is recommendedfor this purpose). ELISA. Western blot.
    Other applications not tested.
    Optimal dilutions are dependent on conditions and should be determined by the user.
    限制
    仅限研究用
  • 浓度
    0.5 mg/mL
    缓冲液
    0.02 M Potassium phosphate, 0.12 M Sodium chloride pH 7.2, 0.01 % Sodium Azide (NaN3)
    储存液
    Sodium azide
    注意事项
    This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    注意事项
    Avoid repeated freezing and thawing. Should this product contain a precipitate we recommend microcentrifugation before use.
    储存条件
    4 °C/-20 °C
    储存方法
    Store the antibody undiluted at 2 - 8 °C up to one month or (in aliquots) at -20 °C for longer.
  • 抗原
    HA-Tag
    别名
    HA Epitope Tag (YPYDVPDYA) (HA-Tag 产品)
    物质类
    Tag
    背景
    Human influenza hemagglutinin (HA) is a surface glycoprotein required for the infectivity of the human virus. The HA tag is derived from the HA molecule corresponding to amino acids 98-106 has been extensively used as a general epitope tag in expression vectors. Many recombinant proteins have been engineered to express the HA tag, which does not appear to interfere with the bioactivity or the biodistribution of the recombinant protein. This tag facilitates the detection, isolation, and purification of the proteins.
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