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- 抗原 See all CHEK2 抗体
- CHEK2 (Checkpoint Kinase 2 (CHEK2))
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抗原表位
- N-Term
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适用
- 人
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宿主
- 兔
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克隆类型
- 多克隆
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标记
- This CHEK2 antibody is un-conjugated
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应用范围
- Western Blotting (WB), Immunohistochemistry (IHC)
- 序列
- MSRESDVEAQ QSHGSSACSQ PHGSVTQSQG SSSQSQGISS SSTSTMPNSS
- 预测反应
- Human: 100%
- 产品特性
- This is a rabbit polyclonal antibody against CHEK2. It was validated on Western Blot using a cell lysate as a positive control.
- 纯化方法
- Affinity Purified
- 免疫原
- The immunogen is a synthetic peptide directed towards the N terminal region of human CHEK2
- Top Product
- Discover our top product CHEK2 Primary Antibody
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- 应用备注
- Optimal working dilutions should be determined experimentally by the investigator.
- 说明
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Antigen size: 514 AA
- 限制
- 仅限研究用
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- 状态
- Liquid
- 浓度
- Lot specific
- 缓冲液
- Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.
- 储存液
- Sodium azide
- 注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 注意事项
- Avoid repeated freeze-thaw cycles.
- 储存条件
- -20 °C
- 储存方法
- For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.
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Parkinsonism and midbrain dysfunction after shunt placement for obstructive hydrocephalus." in: Journal of clinical neuroscience : official journal of the Neurosurgical Society of Australasia, Vol. 13, Issue 3, pp. 373-8, (2006) (PubMed).
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Parkinsonism and midbrain dysfunction after shunt placement for obstructive hydrocephalus." in: Journal of clinical neuroscience : official journal of the Neurosurgical Society of Australasia, Vol. 13, Issue 3, pp. 373-8, (2006) (PubMed).
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- 抗原
- CHEK2 (Checkpoint Kinase 2 (CHEK2))
- 别名
- CHEK2 (CHEK2 产品)
- 别名
- CDS1 antibody, CHK2 antibody, HuCds1 antibody, LFS2 antibody, PP1425 antibody, RAD53 antibody, hCds1 antibody, fa66f08 antibody, wu:fa66f08 antibody, zgc:55865 antibody, Cds1 antibody, HUCDS1 antibody, Rad53 antibody, Chk2 antibody, cds1 antibody, chek2 antibody, chk2 antibody, hucds1 antibody, lfs2 antibody, pp1425 antibody, rad53 antibody, checkpoint kinase 2 antibody, serine/threonine-protein kinase chk2 antibody, checkpoint kinase 2 L homeolog antibody, CHEK2 antibody, chek2 antibody, Chek2 antibody, MCYG_07308 antibody, chek2.L antibody
- 背景
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In response to DNA damage and replication blocks, cell cycle progression is halted through the control of critical cell cycle regulators. CHEK2 is a cell cycle checkpoint regulator and putative tumor suppressor. It contains a forkhead-associated protein interaction domain essential for activation in response to DNA damage and is rapidly phosphorylated in response to replication blocks and DNA damage. When activated, CHEK2 is known to inhibit CDC25C phosphatase, preventing entry into mitosis, and has been shown to stabilize the tumor suppressor protein p53, leading to cell cycle arrest in G1. In addition, this protein interacts with and phosphorylates BRCA1, allowing BRCA1 to restore survival after DNA damage. Mutations in its gene have been linked with Li-Fraumeni syndrome, a highly penetrant familial cancer phenotype usually associated with inherited mutations in TP53. Also, mutations in its gene are thought to confer a predisposition to sarcomas, breast cancer, and brain tumors. This nuclear protein is a member of the CDS1 subfamily of serine/threonine protein kinases.In response to DNA damage and replication blocks, cell cycle progression is halted through the control of critical cell cycle regulators. The protein encoded by this gene is a cell cycle checkpoint regulator and putative tumor suppressor. It contains a forkhead-associated protein interaction domain essential for activation in response to DNA damage and is rapidly phosphorylated in response to replication blocks and DNA damage. When activated, the encoded protein is known to inhibit CDC25C phosphatase, preventing entry into mitosis, and has been shown to stabilize the tumor suppressor protein p53, leading to cell cycle arrest in G1. In addition, this protein interacts with and phosphorylates BRCA1, allowing BRCA1 to restore survival after DNA damage. Mutations in this gene have been linked with Li-Fraumeni syndrome, a highly penetrant familial cancer phenotype usually associated with inherited mutations in TP53. Also, mutations in this gene are thought to confer a predisposition to sarcomas, breast cancer, and brain tumors. This nuclear protein is a member of the CDS1 subfamily of serine/threonine protein kinases. Three transcript variants encoding different isoforms have been found for this gene.
Alias Symbols: RP11-436C9.1, CDS1, CHK2, HuCds1, LFS2, PP1425, RAD53, hCds1
Protein Interaction Partner: SRPK1, PPP2CA, PLK1, CHEK2, PRKDC, ATM, POLR2L, RCHY1, VHL, UBC, NR4A1, ELAVL1, CDC25A, ARHGAP1, PER3, CDC25C, CUL1, CUL4A, KPNA2, APP, STRAP, TP53, BRCA1, RB1, DAPK3, UBR5, PML, MDM2, BRCA2, KAT2B, LATS2, PSME3, TRIM28, RNF8, STAT1, PPP2R5E, PPP2R5D, PPP
Protein Size: 514 - 分子量
- 58 kDa
- 基因ID
- 11200
- NCBI登录号
- NM_145862, NP_665861
- 途径
- p53 Pathway, Apoptosis, Cell Division Cycle
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