HAVCR1 抗体 (Hepatitis A Virus Cellular Receptor 1) (AA 332-348)

Details for Product anti-HAVCR1 Antibody No. ABIN3044226, 供应商: Log in to see
抗原
  • HAVCR1
  • LOC100226241
  • AI503787
  • KIM-1
  • TIM-1
  • Tim1
  • Timd1
  • HAVCR
  • HAVCR-1
  • KIM1
  • TIM
  • TIM1
  • TIMD-1
  • TIMD1
  • Kim1
  • hepatitis A virus cellular receptor 1
  • Rho guanine nucleotide exchange factor (GEF) 5
  • RCJMB04_10h2
  • HAVCR1
  • LOC100226241
  • Havcr1
  • Arhgef5
抗原表位
AA 332-348, C-Term
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适用

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宿主

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克隆类型
多克隆
标记
This HAVCR1 antibody is un-conjugated
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应用范围
Immunocytochemistry (ICC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
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Supplier
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Available images

'独立验证'标志
抗原 HAVCR1
Method validated Immunohistochemistry
Positive Control kidney, colon/rectum
Notes Passed. ABIN3044226 staining is consistent with RNA-seq data and published literature.
Primary Antibody ABIN3044226
Secondary Antibody HRP polymer (ThermoFisher Scientific, TL-125-PH)
实验流程
  • Sample preparation:
    • Fixation and TMA preparation according to PMID: 22688270.
    • Cut 4µm TMA sections using a waterfall microtome (ThermoFisher Scientific, HM355S).
    • Dry sections ON at RT and then back them for 12-24h at 50°C.
  • Deparaffinization and rehydration in xylene and graded alcohol series:
    • xylene twice for 3min.
    • 100% ethanol for 1min.
    • 95% ethanol for 1min.
    • 0.3% H2O2 in 95% ethanol for 5min to block endogenous peroxidase.
    • 70% ethanol for 1min.
    • 50% ethanol for 1min.
    • distilled H2O for 2min.
  • Antigen Retrieval by Heat Induced Epitope Retrieval (HIER):
    • Boil the sections in Lab Vision Citrate buffer pH6.0 (ThermoFisher Scientific, TA-250-PM1X) for 4min at 125°C in a decloaking chamber (Biocare Medical).
    • Allow slides to cool down to 90°C in the decloaking chamber.
  • Immunostaining in a Lab Vision Autostainer 480 (ThermorFisher Scientific) at RT with 300µl of reagents for each step:
    • Rinse sections in Lab Vision wash buffer with extra tween added to a final concentration of 0.2% (ThermoFisher Scientific, TA-999-TT and TA-125-TW) wash buffer.
    • Block sections with Lab Vision Ultra V-Block (ThermoFisher Scientific, TA-125-UB) for 5min.
    • Rinse sections 2x in wash buffer.
    • Incubate sections with primary rabbit anti-HAVCR1 antibody (antibodies-online, ABIN3044226) diluted 1:2000 for 30min.
    • Rinse sections 3x in wash buffer.
    • Incubate sections with labeled HRP polymer (ThermoFisher Scientific, TL-125-PH) for 30min.
    • Rinse sections 2x in wash buffer.
    • Develop in DAB Quanto solution (ThermoFisher Scientific, TL-125-QHDX) for 5min.
    • Rinse sections in distilled H2O.
  • Washing, counterstaining, and coverslipping in Autostainer XL (Leica Biosystems, ST5010) at RT:
    • Counterstaining in Mayer’s hematoxylin plus (HistoLab, 01820) for 7.5min.
    • Rinse sections in tap water for 5min.
    • Rinse sections in lithium carbonate water diluted 1:5 for 5min.
    • Dehydration in graded ethanol series and Neo-Clear (Merck Millipore, 109843) according to the manufacturer recommendations.
    • Automated mounting (Leica Biosystems, CV5030) of coverslip with Pertex (Histolab, 00871).
  • Microscopy:
    • Image acquisition on a Leica Aperio AT2 and Aperio Scanscope AT at 20x magnification.
Experimental Notes Based on published literature, HAVCR1 is widely expressed with highest expected levels in kidney and testis. ABIN3044226 stains kidney and colon/rectum where the protein should be enhanced according to RNA-seq data. Staining in kidney is not distinct but the staining is better in colon/rectum.
Validation Images
Immunohistochemistry validation image for anti-Hepatitis A Virus Cellular Receptor 1 (HAVCR1) (AA 332-348), (C-Term) antibody (ABIN3044226) Staining of human rectum section in a tissue microarray with ABIN3044226. See the pro...
原理 Rabbit IgG polyclonal antibody for Hepatitis A virus cellular receptor 1(HAVCR1) detection. Tested with WB, IHC-P, ICC in Human.
免疫原 A synthetic peptide corresponding to a sequence at the C-terminus of human TIM 1(332-348aa IKALQNAVEKEVQAEDN).
序列 IKALQNAVEK EVQAEDN
亚型 IgG
交叉反应 (详细) No cross reactivity with other proteins.
产品特性 Rabbit IgG polyclonal antibody for Hepatitis A virus cellular receptor 1(HAVCR1) detection. Tested with WB, IHC-P, ICC in Human.
Gene Name: hepatitis A virus cellular receptor 1
Protein Name: Hepatitis A virus cellular receptor 1(HAVcr-1)
纯化方法 Immunogen affinity purified.
别名 HAVCR1 (HAVCR1 Antibody 摘要)
背景 KIM1(KIDNEY INJURY MOLECULE 1), also known as HAVCR1, HAVCR or TIM1, is a protein that in humans is encoded by the KIM1 gene. The KIM1 gene is mapped to 5q33.3. Biochemical, mutational, and cell adhesion analyses confirm that Tim1 is capable of homophilic Tim-Tim interactions. The features identified in murine KIM1 is conserved in human KIM1. The KIM1 protein is indeed a receptor for the virus through the infection of canine osteogenic sarcoma cells expressing HAVCR1 with HAV. Using a monoclonal antibody to mouse Tim1, Tim1 is expressed after activation of naive T cells and on T cells differentiated in Th2-polarizing conditions. Ectopic expression of KIM1 during mouse T-cell differentiation leads to production of the Th2-type cytokine Il4, but not the Th1-type cytokine Ifng. KIM1-expressing epithelial cells internalized apoptotic bodies, and Kim1 is directly responsible for phagocytosis in cultured primary rat tubule epithelial cells and in porcine and canine epithelial cell lines.

Synonyms: HAVCR 1 antibody|HAVcr-1 antibody|HAVCR1 antibody|Hepatitis A virus cellular receptor 1 antibody|Kidney injury molecule 1 antibody|KIM 1 antibody|KIM-1 antibody|T cell immunoglobin domain and mucin domain protein 1 antibody|T-cell immunoglobulin and mucin domain-containing protein 1 antibody|T-cell membrane protein 1 antibody|TIM antibody|TIM-1 antibody|TIM1 antibody|TIMD 1 antibody|TIMD-1 antibody|TIMD1 antibody|TIMD1_HUMAN antibody
UniProt Q96D42
应用备注 WB: Concentration: 0.1-0.5 μg/mL, Tested Species: Human
IHC-P: Concentration: 0.5-1 μg/mL, Tested Species: Human, Epitope Retrieval by Heat: Boiling the paraffin sections in 10 mM citrate buffer, pH 6.0, for 20 mins is required for the staining of formalin/paraffin sections.
ICC: Concentration: 0.5-1 μg/mL, Tested Species: Human
Notes: Tested Species: Species with positive results. Predicted Species: Species predicted to be fit for the product based on sequence similarities. Other applications have not been tested. Optimal dilutions should be determined by end users.
说明

Antibody can be supported by chemiluminescence kit ABIN921124 in WB, supported by ABIN921231 in IHC(P) and ICC.

限制 仅限研究用
状态 Lyophilized
溶解方式 Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.
浓度 500 μg/mL
缓冲液 Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal, 0.05 mg Sodium azide.
储存液 Thimerosal (Merthiolate), Sodium azide
注意事项 This product contains Sodium azide and Thimerosal (Merthiolate): POISONOUS AND HAZARDOUS SUBSTANCES which should be handled by trained staff only.
注意事项 Avoid repeated freezing and thawing.
储存条件 4 °C/-20 °C
储存方法 At -20°C for one year. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20 °C for a longer time. Avoid repeated freezing and thawing.
有效期 12 months
厂商提供的图像
Immunohistochemistry (IHC) image for anti-HAVCR1 抗体 (Hepatitis A Virus Cellular Receptor 1) (AA 332-348) (ABIN3044226) Anti-TIM 1 antibody, IHC(P) IHC(P): Human Tonsil Tissue
Immunohistochemistry (IHC) image for anti-HAVCR1 抗体 (Hepatitis A Virus Cellular Receptor 1) (AA 332-348) (ABIN3044226) Anti-TIM 1 antibody, ICC ICC: K562 Cell
Western Blotting (WB) image for anti-HAVCR1 抗体 (Hepatitis A Virus Cellular Receptor 1) (AA 332-348) (ABIN3044226) Anti-TIM 1 antibody, Western blotting Lane 1: SMMC Cell Lysate Lane 2: HELA Cell Lysa...