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HAVCR1 抗体 (C-Term)

HAVCR1 适用: 人 WB, ICC, IHC (p) 宿主: 兔 Polyclonal unconjugated
产品编号 ABIN3044226
发货至: 中国
  • 抗原 See all HAVCR1 抗体
    HAVCR1 (Hepatitis A Virus Cellular Receptor 1 (HAVCR1))
    抗原表位
    • 12
    • 9
    • 8
    • 6
    • 6
    • 5
    • 5
    • 4
    • 4
    • 3
    • 2
    • 2
    • 2
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    • 1
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    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
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    • 1
    AA 332-348, C-Term
    适用
    • 98
    • 48
    • 34
    • 6
    • 6
    • 5
    宿主
    • 101
    • 24
    • 5
    • 2
    克隆类型
    • 104
    • 28
    • 1
    多克隆
    标记
    • 64
    • 15
    • 14
    • 6
    • 6
    • 4
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    • 2
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    • 1
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    This HAVCR1 antibody is un-conjugated
    应用范围
    • 92
    • 41
    • 32
    • 17
    • 15
    • 12
    • 11
    • 11
    • 10
    • 5
    • 5
    • 3
    • 2
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    • 1
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    • 1
    Western Blotting (WB), Immunocytochemistry (ICC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
    原理
    Rabbit IgG polyclonal antibody for Hepatitis A virus cellular receptor 1(HAVCR1) detection. Tested with WB, IHC-P, ICC in Human.
    序列
    IKALQNAVEK EVQAEDN
    交叉反应 (详细)
    No cross reactivity with other proteins.
    产品特性
    Rabbit IgG polyclonal antibody for Hepatitis A virus cellular receptor 1(HAVCR1) detection. Tested with WB, IHC-P, ICC in Human.
    Gene Name: hepatitis A virus cellular receptor 1
    Protein Name: Hepatitis A virus cellular receptor 1(HAVcr-1)
    纯化方法
    Immunogen affinity purified.
    免疫原
    A synthetic peptide corresponding to a sequence at the C-terminus of human TIM 1(332-348aa IKALQNAVEKEVQAEDN).
    亚型
    IgG
  • 应用备注
    WB: Concentration: 0.1-0.5 μg/mL, Tested Species: Human
    IHC-P: Concentration: 0.5-1 μg/mL, Tested Species: Human, Epitope Retrieval by Heat: Boiling the paraffin sections in 10 mM citrate buffer, pH 6.0, for 20 mins is required for the staining of formalin/paraffin sections.
    ICC: Concentration: 0.5-1 μg/mL, Tested Species: Human
    Notes: Tested Species: Species with positive results. Predicted Species: Species predicted to be fit for the product based on sequence similarities. Other applications have not been tested. Optimal dilutions should be determined by end users.
    说明

    Antibody can be supported by chemiluminescence kit ABIN921124 in WB, supported by ABIN921231 in IHC(P) and ICC.

    限制
    仅限研究用
  • 生效 #300028 (Immunohistochemistry)
    '独立验证'标志
    by
    Human Protein Atlas
    No.
    #300028
    日期
    2016.12.13
    抗原
    HAVCR1
    Lot Number
    Method validated
    Immunohistochemistry
    Positive Control
    kidney, colon/rectum
    Negative Control
    Notes
    Passed. ABIN3044226 staining is consistent with RNA-seq data and published literature.
    '独立验证'标志
    Validation Images
    Full Methods
    Primary Antibody
    ABIN3044226
    Secondary Antibody
    HRP polymer (ThermoFisher Scientific, TL-125-PH)
    Full Protocol
    • Sample preparation:
      • Fixation and TMA preparation according to PMID: 22688270.
      • Cut 4µm TMA sections using a waterfall microtome (ThermoFisher Scientific, HM355S).
      • Dry sections ON at RT and then back them for 12-24h at 50°C.
    • Deparaffinization and rehydration in xylene and graded alcohol series:
      • xylene twice for 3min.
      • 100% ethanol for 1min.
      • 95% ethanol for 1min.
      • 0.3% H2O2 in 95% ethanol for 5min to block endogenous peroxidase.
      • 70% ethanol for 1min.
      • 50% ethanol for 1min.
      • distilled H2O for 2min.
    • Antigen Retrieval by Heat Induced Epitope Retrieval (HIER):
      • Boil the sections in Lab Vision Citrate buffer pH6.0 (ThermoFisher Scientific, TA-250-PM1X) for 4min at 125°C in a decloaking chamber (Biocare Medical).
      • Allow slides to cool down to 90°C in the decloaking chamber.
    • Immunostaining in a Lab Vision Autostainer 480 (ThermorFisher Scientific) at RT with 300µl of reagents for each step:
      • Rinse sections in Lab Vision wash buffer with extra tween added to a final concentration of 0.2% (ThermoFisher Scientific, TA-999-TT and TA-125-TW) wash buffer.
      • Block sections with Lab Vision Ultra V-Block (ThermoFisher Scientific, TA-125-UB) for 5min.
      • Rinse sections 2x in wash buffer.
      • Incubate sections with primary rabbit anti-HAVCR1 antibody (antibodies-online, ABIN3044226) diluted 1:2000 for 30min.
      • Rinse sections 3x in wash buffer.
      • Incubate sections with labeled HRP polymer (ThermoFisher Scientific, TL-125-PH) for 30min.
      • Rinse sections 2x in wash buffer.
      • Develop in DAB Quanto solution (ThermoFisher Scientific, TL-125-QHDX) for 5min.
      • Rinse sections in distilled H2O.
    • Washing, counterstaining, and coverslipping in Autostainer XL (Leica Biosystems, ST5010) at RT:
      • Counterstaining in Mayer’s hematoxylin plus (HistoLab, 01820) for 7.5min.
      • Rinse sections in tap water for 5min.
      • Rinse sections in lithium carbonate water diluted 1:5 for 5min.
      • Dehydration in graded ethanol series and Neo-Clear (Merck Millipore, 109843) according to the manufacturer recommendations.
      • Automated mounting (Leica Biosystems, CV5030) of coverslip with Pertex (Histolab, 00871).
    • Microscopy:
      • Image acquisition on a Leica Aperio AT2 and Aperio Scanscope AT at 20x magnification.
    Experimental Notes
    Based on published literature, HAVCR1 is widely expressed with highest expected levels in kidney and testis. ABIN3044226 stains kidney and colon/rectum where the protein should be enhanced according to RNA-seq data. Staining in kidney is not distinct but the staining is better in colon/rectum.
  • 状态
    Lyophilized
    溶解方式
    Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.
    浓度
    500 μg/mL
    缓冲液
    Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal, 0.05 mg Sodium azide.
    储存液
    Thimerosal (Merthiolate), Sodium azide
    注意事项
    This product contains Sodium azide and Thimerosal (Merthiolate): POISONOUS AND HAZARDOUS SUBSTANCES which should be handled by trained staff only.
    注意事项
    Avoid repeated freezing and thawing.
    储存条件
    4 °C/-20 °C
    储存方法
    At -20°C for one year. After reconstitution, at 4°C for one month.
    It can also be aliquotted and stored frozen at -20 °C for a longer time. Avoid repeated freezing and thawing.
    有效期
    12 months
  • 抗原
    HAVCR1 (Hepatitis A Virus Cellular Receptor 1 (HAVCR1))
    别名
    HAVCR1 (HAVCR1 产品)
    别名
    HAVCR antibody, HAVCR-1 antibody, KIM-1 antibody, KIM1 antibody, TIM antibody, TIM-1 antibody, TIM1 antibody, TIMD-1 antibody, TIMD1 antibody, Kim1 antibody, HAVCR1 antibody, LOC100226241 antibody, AI503787 antibody, Tim1 antibody, Timd1 antibody, hepatitis A virus cellular receptor 1 antibody, hepatitis A virus cellular receptor 1 homolog antibody, HAVCR1 antibody, Havcr1 antibody, LOC100226241 antibody
    物质类
    Virus
    背景
    KIM1(KIDNEY INJURY MOLECULE 1), also known as HAVCR1, HAVCR or TIM1, is a protein that in humans is encoded by the KIM1 gene. The KIM1 gene is mapped to 5q33.3. Biochemical, mutational, and cell adhesion analyses confirm that Tim1 is capable of homophilic Tim-Tim interactions. The features identified in murine KIM1 is conserved in human KIM1. The KIM1 protein is indeed a receptor for the virus through the infection of canine osteogenic sarcoma cells expressing HAVCR1 with HAV. Using a monoclonal antibody to mouse Tim1, Tim1 is expressed after activation of naive T cells and on T cells differentiated in Th2-polarizing conditions. Ectopic expression of KIM1 during mouse T-cell differentiation leads to production of the Th2-type cytokine Il4, but not the Th1-type cytokine Ifng. KIM1-expressing epithelial cells internalized apoptotic bodies, and Kim1 is directly responsible for phagocytosis in cultured primary rat tubule epithelial cells and in porcine and canine epithelial cell lines.

    Synonyms: HAVCR 1 antibody|HAVcr-1 antibody|HAVCR1 antibody|Hepatitis A virus cellular receptor 1 antibody|Kidney injury molecule 1 antibody|KIM 1 antibody|KIM-1 antibody|T cell immunoglobin domain and mucin domain protein 1 antibody|T-cell immunoglobulin and mucin domain-containing protein 1 antibody|T-cell membrane protein 1 antibody|TIM antibody|TIM-1 antibody|TIM1 antibody|TIMD 1 antibody|TIMD-1 antibody|TIMD1 antibody|TIMD1_HUMAN antibody
    UniProt
    Q96D42
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